Prevalence and antibiotic resistance of Enterococcus strains isolated from poultry

Stępień–Pyśniak, Dagmara; Marek, Agnieszka; Banach, Tomasz; Adaszek, Łukasz; Pyzik, Ewelina; Wilczyński, Jarosław; Winiarczyk, S.

doi:10.1556/004.2016.016

Cited by 61 publications

(45 citation statements)

References 39 publications

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“…We found that E. faecalis seems to be the dominant enterococcal species in poultry. Our results were consistent with the literature (15). In opposition to other authors, we isolated E. cecorum more often, and more rarely E. faecium, E. hirae, E. gallinarum.…”

Section: Resultssupporting

confidence: 91%

“…Among other poultry species, strains originated from turkeys (23.4%; 548/2337), geese (2.1%; 50/2337) and ducks (0.7; 16/2337). Similarly to other studies (15), in our study the most positive samples for enterococci were in broilers. In poultry, 9 of the most frequent identified species were E. faecalis (42.5%) > E. cecorum (7.9%) > E. faecium (4.4%) > E. hirae (2.7%) > E. gallinarum (2.2%) >E. casseliflavus (0.6%) > E. durans (0.2%) > E. avium (0.16%) > E. aquimarinus (0.04%).…”

Section: Resultssupporting

confidence: 90%

“…2). Similarly, other authors isolated E. faecalis (then E. faecium) mainly from broilers between 1 and 5 days of age (15,19). We found that E. cecorum may be seen in all groups of poultry, usually after 3.6 weeks of age depending on the purpose of production (Tab.…”

Section: Resultsmentioning

confidence: 75%

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Occurrence of Enterococcus spp. in poultry in Poland based on 2014–2015 data

Dolka

Golebiewska-Kosakowska²,

Krajewski³

et al. 2017

Medycyna Weterynaryjna

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Bacteria of the genus Enterococcus are mainly commensals building natural microflora in the digestive tract of birds and mammals. They belong to the potentially pathogenic microorganisms. Among poultry, infections caused by enterococci were reported in chickens, turkeys, ducks and ostriches. The aim of this study was to evaluate the occurrence of enterococci in poultry in Poland, including identification of enterococcus species composition and determination of the age of birds. The analysis was based on data obtained from 2014 – 2015 from Division of Avian Diseases at Warsaw University of Life Sciences-SGGW and four veterinary laboratories in Poland: Lab – Vet, Tarnowo Podgórne; SLW Biolab, Ostróda; Vetdiagnostica, Solec Kujawski; Vet – Lab Brudzew. Seven enterococcal species were isolated from broiler chickens (CB), commercial layers (CL), and broiler breeder flocks (BB), nine from all poultry types (chickens, turkey, ducks and geese). The most often isolated enterococci from CB were E. faecalis (57%) > E. cecorum (7%) > E. faecium (5.2%) > E. hirae (3.6%) > E. gallinarum (2.5%) > E. casseliflavus (0.7%) > E. durans (0.2%). Seven Enterococcus species were isolated from sources associated with poultry, most often E. faecalis > E. faecium > E. cecorum > E. hirae. The differences in the occurrence of particular enterococcal species were observed between CB, BB and CL. The mean age at the time of isolation of E. cecorum was approx.: 3.6 weeks in CB, 27.5 weeks in BB, 33.3 weeks in CL, 12.9 weeks in turkeys, 3.6 weeks in ducks, 39.5 weeks in geese. E. faecalis and E. faecium dominated in samples obtained from hatching eggs, dead-in-shell embryos and from samples related to poultry environment. In conclusion, this study indicates the high prevalence of bacteria of the Enterococcus genus in poultry. The present findings demonstrate the differences in Enterococcus species between poultry groups, including with regard to age. In total 10 enterococcal species (E. faecalis, E. cecorum, E. hirae, E. faecium, E. gallinarum, E. casseliflavus, E. durans, E. avium E. thailandicus, E. aquimarinus) were detected in poultry, poultry environmental samples, hatching eggs and dead-in-shell embryos. Enterococcus faecalis and E. cecorum were found in all above-mentioned sources.

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Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 90%

See 1 more Smart Citation

Occurrence of Enterococcus spp. in poultry in Poland based on 2014–2015 data

Dolka

Golebiewska-Kosakowska²,

Krajewski³

et al. 2017

Medycyna Weterynaryjna

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“…Although enterococci are usually thought to be harmless commensals, they can also act as opportunistic pathogens. In recent years, their increased role has been noted in poultry and pigeon pathology [6][7][8][9]. According to the literature the drug-resistance of the bacteria isolated from pigeons may be very high [4,10].…”

Section: Introductionmentioning

confidence: 99%

“…Multidrug-resistant enterococci have emerged as a global threat to public health, and vancomycin-resistant enterococci (VRE) currently rank among the leading bacterial causes of nosocomial infections. Although several studies reported the antibiotic resistance in avian Enterococcus [8,11,12] and the enterococcal virulence factors in humans, poultry, farm animals and wildlife, food, environment [11,[13][14][15][16][17], so far little is known about the antimicrobial susceptibility of enterococci from pigeons [4,18]. Moreover the occurrence of virulence factors has not been described among Enterococcus spp.…”

Section: Introductionmentioning

confidence: 99%

Prevalence, antibiotic susceptibility and virulence factors of Enterococcus species in racing pigeons (Columba livia f. domestica)

et al. 2020

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Background: This study was aimed to investigate the intestinal microbiota in racing pigeons with regard to Enterococcus species distribution, virulence factors and antibiotic susceptibility. Three methods (API, Multiplex sodA-PCR, 16S rRNA sequencing) were compared for Enterococcus species identification. Cloacal samples from 179 apparently healthy pigeons of 13 different flocks were tested. Results: Multiplex sodA-PCR and 16S rRNA gene sequencing showed almost perfect agreement in Enterococcus species identification. Isolates were identified as Enterococcus columbae (34.5%), Enterococcus hirae (20.7%), Enterococcus faecalis (11.7%), Enterococcus faecium (11.7%), Enterococcus gallinarum (9%), Enterococcus mundtii (4.8%), Enterococcus casseliflavus (3.4%), Enterococcus cecorum (2.1%), Enterococcus durans (2.1%). More Enterococcus species were found after the race season than before. The study showed differences between Enterococcus species in relation to 68.8% (22/32) biochemical parameters. Six out of seven virulence genes were detected: gelE (43.5%), asa1 (42.1%), efaA (30.3%), ace (30.3%), cylA (27.6%), and esp (9%). None of the isolates harboured hyl gene. Overall 15.2% of Enterococcus isolates produced gelatinase, but 66.7% gelE genes were silent. Enterococcus faecalis showed the most often efaA, ace and gelatinase activity than other enterococcal species. Nearly all isolates (93.1%) were resistant to at least one antibiotic. The most frequent resistance was to enrofloxacin (80%), doxycycline with teicoplanin (73.1%), erythromycin (49.7%). The study revealed significant differences between some enterococcal species in the antibiotic susceptibility to different antibiotics. Enterococcus columbae and E. cecorum showed significantly more frequent resistance to chloramphenicol than other enterococci. The presence of VRE (19.3%), HLGR (2.8%) and no LRE were found. Overall 30.3% of isolates were positive for vancomycin resistance genes, where vanC1 (E. gallinarum), vanC2-C3 (E. hirae, E. casseliflavus), vanB (E. columbae) predominated. Conclusions: We conclude, that intestinal microbiota in racing pigeons is composed by 9 different Enterococcus species. Given that racing pigeons are kept in close contact with humans and backyard animals, combined with their long-distance flight abilities, they can serve as potential source of virulent and antibiotic resistant Enterococcus spp. in the environment.

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