Identification of steroidal 5,8-peroxides by gas-liquid chromatography

Ga, Blondin; Bd, Kulkarni; John, John P.; Rt, Van Aller; Pt, Russell; Wr, Nes

doi:10.1021/ac60245a004

Cited by 11 publications

(1 citation statement)

References 18 publications

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“…The biphase column used in the previous studies of Nair et al (14) was used in this study, except that 2.0 mg of ubiquinones0 per 25 g of inert support was added. Blondin et al (24) observed that the 5,8-peroxides of ergosterol and 7-dehydrocholesterol gave a characteristic pattern of pyrolysis products in contrast to other sterols, which gave single peaks on 0.92% XE-60 columns. The 6 ft column was packed with 0.92% XE-60 on 90-100 mesh Anakrom AB maintained isothermally at ca.…”

Section: Vl Tami N Dmentioning

confidence: 99%

Gas chromatography of the fat‐soluble vitamins: A review

Sheppard

Prosser

Hubbard

1972

J Americ Oil Chem Soc

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The application of gas liquid chromatography (GLC) as an analytical tool for the determination of the fat-soluble vitamins A, D, E and K has yet to be utilized to its full potential. A review of the published work of many researchers in this field is presented. GLC methods to measure the vitamin A isomers have not been developed to any appreciable practical extent. Liquid liquid chromatography might well be the technique of choice. In the field of vitamin D there are indications that a practical GLC analysis is feasible for pharmaceutical preparations. The GLC applications for vitamin E are diverse, well defined and generally widely accepted in research laboratory situations and for regulatory and quality control usage. Vitamins K 1 and K 2 have been measured with limited success in a few research laboratories, but the GLC methods have not developed on a practical basis. However GLC is used for measuring vitamin K 3 (menadione and menadione sodium bisulfite) on a fairly routine basis in quality control laboratories.

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Section: Vl Tami N Dmentioning

confidence: 99%

Gas chromatography of the fat‐soluble vitamins: A review

Sheppard

Prosser

Hubbard

1972

J Americ Oil Chem Soc

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The lipids of the common house cricket,Acheta domesticus L. III. Sterols

Martin

Carls

1968

Lipids

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Sterols constitute 1.95% of the total extractable lipids ofAcheta domesticus L., of which 18% are esterified. The free sterols consist of cholestane-3beta-ol (0.5%), Delta(5)-cholestene-3beta-ol (83.5%), Delta(7)-cholestene-3beta-ol (2.3%) Delta(5,7)-cholestadiene-3beta-ol (3%), Delta(5,22)-cholestadiene-3beta-ol (4%), Delta(5,7,22)-cholestatriene-3beta-ol (0.2%), campestane-3beta-ol (0.03%), Delta(5)-campestene-3beta-ol (1.0%), Delta(7)-campestene-3beta-ol (trace), Delta(5,7)-campestadiene-3beta-ol (0.2%), stigmastane-3beta-ol (0.09%), Delta(5)-stigmastene-3beta-ol (2.1%), Delta(7)-stigmastene-3beta-ol (0.04%), Delta(5,7)-stigmastadiene-3beta-ol (0.4%), Delta(5,22)-stigmastadiene-3betaol (0.1%). The same sterols are present in the esterified sterol fraction. Delta(7)-Sterols and Delta(5,7)-sterols are present in significantly larger amounts in the esterified fraction than in the free sterol fraction. By a comparison with the sterols of the cricket food, it is clear thatA. domesticus is capable of removing methyl and ethyl groups from C-24 of sterols of the campestane and stigmastane type. The ability to introduce a Delta(7) double bond into saturated and Delta(5)-sterols is indicated, and it is suggested that Delta(7)-sterols of the C(27), C(28), and C(29) sterol series may be intermediates in the conversion of Delta(5)-sterols to Delta(5,7)-sterols.

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