Identification of Staphylococcus spp. using (GTG)5-PCR fingerprinting

Švec, Pavel; Pantůček, Roman; Petřáš, Petr; Sedláček, Ivo; Nováková, Dana

doi:10.1016/j.syapm.2010.09.004

Cited by 38 publications

(23 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The molecular fingerprinting of L. garvieae strains was determined using RAPD and REP-PCR with BOXA1R and (GTG) 5 primers. These methods, which use short arbitrary primers or primers targeting short repetitive sequences interspersed throughout the genome are an established approach for delineation of bacteria at the species and strain-level (Randazzo et al, 2009;Švec et al, 2010). The discriminatory power of these primer sets was similar, with 20 different profiles obtained by BOXA1R and (GTG) 5 and 23 different profiles obtained by M13 for a collection of 49 strains.…”

Section: Resultsmentioning

confidence: 97%

Genetic investigation within Lactococcus garvieae revealed two genomic lineages

Ferrario

Ricci

Borgo

et al. 2012

FEMS Microbiol Lett

View full text Add to dashboard Cite

The diversity of a collection of 49 Lactococcus garvieae strains, including isolates of dairy, fish, meat, vegetable and cereal origin, was explored using a molecular polyphasic approach comprising PCR-ribotyping, REP and RAPD-PCR analyses and a multilocus restriction typing (MLRT) carried out on six partial genes (atpA, tuf, dltA, als, gapC, and galP). This approach allowed high-resolution cluster analysis in which two major groups were distinguishable: one group included dairy isolates, the other group meat isolates. Unexpectedly, of the 12 strains coming from fish, four grouped with dairy isolates, whereas the others with meat isolates. Likewise, strains isolated from vegetables allocated between the two main groups. These findings revealed high variability within the species at both gene and genome levels. The observed genetic heterogeneity among L. garvieae strains was not entirely coherent with the ecological niche of origin of the strains, but rather supports the idea of an early separation of L. garvieae population into two independent genomic lineages.

show abstract

Section: Resultsmentioning

confidence: 97%

Genetic investigation within Lactococcus garvieae revealed two genomic lineages

Ferrario

Ricci

Borgo

et al. 2012

FEMS Microbiol Lett

View full text Add to dashboard Cite

show abstract

“…Genotypic screening of the isolates was performed using rep-PCR fingerprinting with the (GTG) 5 primer, reported as a suitable tool for the identification of Staphylococcus spp. (Svec et al 2010). The resulting fingerprints were processed using BioNumerics v. 7.5 software (Applied-Maths, Kortrijk, Belgium) and compared to the in-house (GTG) 5 -PCR fingerprints database (Svec et al 2010).…”

Section: Methodsmentioning

confidence: 99%

“…(Svec et al 2010). The resulting fingerprints were processed using BioNumerics v. 7.5 software (Applied-Maths, Kortrijk, Belgium) and compared to the in-house (GTG) 5 -PCR fingerprints database (Svec et al 2010).…”

Section: Methodsmentioning

confidence: 99%

Characterisation of methicillin-susceptible Staphylococcus pseudintermedius isolates from canine infections and determination of virulence factors using multiplex PCR

Melter¹,

Švec²,

Tkadlec³

et al. 2017

Vet. Med. - Czech

View full text Add to dashboard Cite

ABSTRACT:Staphylococcus pseudintermedius is a genuine opportunistic pathogen of the skin, especially in canids. However, characterisation of virulence, antimicrobial resistance and genotypic variability in methicillin-susceptible S. pseudintermedius isolates has not been fully explored. In this study, coagulase-positive staphylococcal isolates collected from dogs of various breeds and ages suffering from dermatitis (n = 70), pyoderma (n = 7), and otitis (n = 7), from districts of Prague (Czech Republic) and surrounding areas, were characterised using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry, and repetitive sequence-based PCR fingerprinting. Susceptibility to antimicrobial agents was determined, virulence factor genes for leukocidin (lukSF-I), exfoliatins (exi, expB, and siet), enterotoxin C (sec canine ) and enterotoxin-related genes (se-int and sel) were detected using multiplex PCR and the genotypes of S. pseudintermedius isolates were determined using SmaI macrorestriction analysis. The majority of the staphylococcal isolates (n = 84) were identified as S. pseudintermedius (n = 79) and all of them were susceptible to methicillin/oxacillin (MSSP). About half of the strains (n = 41) were resistant to macrolide-lincosamide-streptogramin B antimicrobial agents and resistance was mediated in all but one of the strains by the erm(B) gene. The genes for lukSF-I, siet, se-int, and sel were detected in the majority of the MSSP strains (96.2%, 100%, 100%, and 73.4%, respectively). Investigated canine S. pseudintermedius isolates were highly heterogeneous, which prevented the correlation of any specific lineage to a particular infection, dog breed, or region of origin. Keywords: Staphylococcus pseudintermedius; macrolide lincosamide-streptogramin B (MLS B ) resistance; genotyping; virulence genesStaphylococcus pseudintermedius, a pathogen that infects dogs, is comparable to Stapylococcus aureus in humans. This analogy fits not only the phenotypic characteristics (e.g. similar colony morphology, pigment, haemolysis), but is also supported by the striking similarity in ecology/epidemiology (colonisation or infection of the skin and skin adnexa, vertical/horizontal transmission), expression of homologous virulence factors (cell wall-anchored proteins such as microbial surface components recognising adhesive matrix molecules; protein A; and enzymes, e.g. coagulase; secreted toxins, such as cytotoxins, exfoliative toxins, or superantigens) and pathogenicity (skin infections or invasive infections). These two coagulase-positive species probably evolved separately through adaptation

show abstract

“…The genomic DNA G+C content of this strain and F. mortiferum, F. necrogenes and C. rectum were determined [9] and DNA-DNA hybridizations were performed [14]. Repetitive sequence-based PCR fingerprinting with the (GTG) 5 primer [39] was also performed to confirm the non-clonal nature of the 9 isolates.…”

Section: Genotypic Characterizationmentioning

confidence: 99%

Detection, isolation and characterization of Fusobacterium gastrosuis sp. nov. colonizing the stomach of pigs

Witte

Flahou

Ducatelle

et al. 2017

Systematic and Applied Microbiology

View full text Add to dashboard Cite

SummaryNine strains of a novel Fusobacterium sp. were isolated from the stomach of 6-8 months old and adult pigs. The isolates were obligately anaerobic, although they endured 2 hours exposure to air. Phylogenetic analysis based on 16S rRNA and gyrase B genes demonstrated that the isolates showed high sequence similarity with Fusobacterium mortiferum, Fusobacterium ulcerans, Fusobacterium varium, Fusobacterium russii and Fusobacterium necrogenes, but formed a distinct lineage in the genus Fusobacterium. Comparative analysis of the genome of the type strain of this novel Fusobacterium sp. confirmed that it is different from other recognized Fusobacterium spp. DNA-DNA hybridization, fingerprinting and genomic %GC determination further supported the conclusion that the isolates belong to a new, distinct species. The isolates were also distinguishable from these and other Fusobacterium spp. by phenotypical characterization. The strains produced indole and exhibited proline arylamidase and glutamic acid decarboxylase activity. They did not hydrolyse esculin, did not exhibit pyroglutamic acid arylamidase, valine arylamidase, α-galactosidase, β-galactosidase, β-galactosidase-6-phosphate or α-glucosidase activity nor produced acid from cellobiose, glucose, lactose, mannitol, mannose, maltose, raffinose, saccharose, salicin or trehalose. The major fatty acids were C16 : 0 and C18 : 1ω9c. The name Fusobacterium gastrosuis sp. nov. is proposed for the novel isolates with the type strain CDW1(T) (= DSM 101753(T) = LMG 29236(T)). We also demonstrated that Clostridium rectum and Fusobacterium mortiferum represent the same species, with nomenclatural priority for the latter.

show abstract

Identification of Staphylococcus spp. using (GTG)5-PCR fingerprinting

Cited by 38 publications

References 33 publications

Genetic investigation within Lactococcus garvieae revealed two genomic lineages

Genetic investigation within Lactococcus garvieae revealed two genomic lineages

Characterisation of methicillin-susceptible Staphylococcus pseudintermedius isolates from canine infections and determination of virulence factors using multiplex PCR

Detection, isolation and characterization of Fusobacterium gastrosuis sp. nov. colonizing the stomach of pigs

Contact Info

Product

Resources

About