Identification of Staphylococci from Bovine Milk in Argentina

Rampone, Horacio; Bogni, Cristina; Giraudo, José; Calzolari, A.

doi:10.1016/s0934-8840(11)80426-3

Cited by 14 publications

(6 citation statements)

References 13 publications

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“…Bacteriological assays were performed according to the criteria of the National Mastitis Council (2004). The presence of bacterial strains of the Staphylococcus genus was determined using 10 μl of milk plated onto blood-agar (TSA with 5% of sheep blood) and incubated at 37 °C for 24 h. The Gram-positive cocci were characterised by standard biochemical tests (Rampone et al, 1993). The SCC was performed with a Somacount 300 (Bentley Instruments, Chaska, MN, USA) according to the revised protocol of the standard fluoro-opto-electronic method (method C, IDF 148A; IDF, 1995).…”

Section: Milk Sampling and Analysismentioning

confidence: 99%

Bovine mastitis prevention: humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period

Pellegrino

Berardo

Giraudo

et al. 2017

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The use of lactic acid bacteria (LAB) in animal feed, constitute an alternative tool for bovine mastitis prevention. Previously, two LAB strains were isolated from bovine milk and selected for their probiotics properties. So far, immune response of inoculating LAB in bovine udders at dry-off period has not been investigated. The immunoglobulin isotype levels and memory cell proliferation in blood and milk of animals inoculated with Lactobacillus lactis subsp. lactis CRL1655 and Lactobacillus perolens CRL1724 at dry-off period was studied. Ten animals were inoculated intramammarily with 10 cells of each LAB (IG) and 2 animals used as control (NIG). Milk and blood samples were taken before inoculation and 1, 2, 4, 6, 12 and 24 h and 7 and 14 days after inoculation. Somatic cell count (SCC) in milk, the presence of bovine mastitis pathogens, the levels of antibodies and lymphocyte proliferation were determined. In the IG, the SCC was <250,000 cells/ml up to 4 h after intramammary inoculation. Six and 12 h after inoculation, the SCC increased up to 600,000 and 2,000,000 cells/ml, respectively. In the NIG, the SCC reached the maximum value 7 days after inoculation. Microbiological analysis showed that all samples were negative for major bovine mastitis pathogens after 24-48 h of incubation. In general, LAB inoculation increased the amount of IgG isotypes in blood and milk, and these antibodies were able to recognise Staphylococcus aureus epitopes. Lymphocytes proliferation was significantly higher in the IG at all time points assayed, following LAB or S. aureus stimulation. The lymphocytes of animals inoculated with LAB do not react in vitro to the presence of S. aureus antigen.. The results showed that probiotic microorganisms could be a natural and effective alternative in the prevention of bovine mastitis at dry-off period and act as immunomodulatory stimulating local and systemic defence lines.

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Section: Milk Sampling and Analysismentioning

confidence: 99%

Bovine mastitis prevention: humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period

Pellegrino

Berardo

Giraudo

et al. 2017

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“…The coagulase-negative (CN) species Staphylococcus caprae was described in 1983 (6), and S. caprae strains have been isolated from goat milk (5,21) but never from cow or sheep milk (5,17). Two human clinical isolates of S. caprae have been reported: one from the pus of a patient with dermatitis and the other from the urine of a patient hospitalized ''with urinary tract infection''.…”

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confidence: 99%

Identification and ribotypes of Staphylococcus caprae isolates isolated as human pathogens and from goat milk

et al. 1995

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We report five cases of human infection with Staphylococcus caprae. Two were community acquired (one case each of endocarditis and urinary tract infection); the other three were acquired in a hospital (two cases of bacteremia associated with intravenous access and one case of urinary tract infection). Analysis of human isolates and goat isolates from eight herds showed that they could be misidentified by some commercial identification systems but were clearly identified as S. caprae by ribotyping, according to their species-specific ribotype. Phylogenetic methods applied to the ribotypes did not reveal two distinct lineages corresponding to the goat and human origins of the isolates, although human ribotypes were clearly distinguishable by the presence of a core of four specific bands. The latter observation may reflect some degree of evolutionary change within the species between human and goat isolates.

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“…Nearly similar result obtained by El-kholy et al,(1994) who recorded that the incidence reached to (22.9%). Baudet&chieze (1994) recorded that the incidence reached to (30%) of the examined milk samples, Rampone et al,(1993) found that Staph. aureus constituted (38.5%) of the isolates obtained from raw milk samples, Saini et al,(1994) found that Staph.…”

Section: Discussionmentioning

confidence: 96%

Bacteriological and molecular studies on staphylococcus aureus isolated from raw milk

Abdel-Tawab

El-Roos

Elgendy

2015

Benha Veterinary Medical Journal

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A B S T R A C TThe present study was performed on a total of 100 raw buffalo Milk samples from different sources at El-Menofiya Governorate that the inspected samples were taken in ice box within an a hour for bacteriological examination. The results revealed that 80 out of 100 samples (80%) were positive for staphylococcus spp. Out of those 80 samples, 42(53%) were coagulase positive Staphylococci spp while 38(47%) were coagulase Negative Staphylococci spp. Out of those 42 coagulase positive Staphylococci spp,31 were (73.8%) Staph. aureus. In vitro Gentamycin, Trimethoprim / sulphamethazole, Ampicillin, Cephadrine were the most proper antibiotic against isolated Staph. aureus. By using PCR Spa virulence gene was detected in two studied strains while, enterotoxin E was detected only in one strain out of 6 studied strains. Meanwhile, hIyA and enterotoxin (Sea, Seb, Sec, Sed) virulence genes were not detected in all studied strains.

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Identification of Staphylococci from Bovine Milk in Argentina

Cited by 14 publications

References 13 publications

Bovine mastitis prevention: humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period

Bovine mastitis prevention: humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period

Identification and ribotypes of Staphylococcus caprae isolates isolated as human pathogens and from goat milk

Bacteriological and molecular studies on staphylococcus aureus isolated from raw milk

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