Bovine mastitis prevention: humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period

Pellegrino, Matías Santiago; Berardo, N; Giraudo, José; Nader‐Macías, María Elena Fátima; Bogni, Cristina

doi:10.3920/bm2016.0194

Cited by 45 publications

(45 citation statements)

References 24 publications

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“…However, rather than reconstituting the microbiota equilibrium, some authors argue that this might be due to an immune stimulation enabling a response of the mammary gland and clearance of subclinical intramammary infection agents (Crispie et al, 2008;Mignacca et al, 2017). In line with this concept, Pellegrino et al (2017) described that inoculation of lactic acid bacteria in cows at dry-off period increased the amount of IgG isotypes in blood and milk and found that these antibodies were able to recognize S. aureus epitopes. Lymphocyte proliferation was significantly higher in the inoculated group at all time points assayed, following lactic acid bacteria or S. aureus stimulation.…”

Section: Can We Manipulate the Milk Microbiota In Order To Improve Mamentioning

confidence: 99%

“…Lymphocyte proliferation was significantly higher in the inoculated group at all time points assayed, following lactic acid bacteria or S. aureus stimulation. The results showed that probiotics could be a natural and effective alternative in the prevention of bovine mastitis at dryoff period and act as an immunomodulator stimulating local and systemic defense lines (Pellegrino et al, 2017). On the other hand, other studies suggest that intramammary probiotics should be considered with caution, although teat apex probiotics deserve further research (Rainard and Foucras, 2018).…”

Section: Can We Manipulate the Milk Microbiota In Order To Improve Mamentioning

confidence: 99%

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Milk Microbiota: What Are We Exactly Talking About?

et al. 2020

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The development of powerful sequencing techniques has allowed, albeit with some biases, the identification and inventory of complex microbial communities that inhabit different body sites or body fluids, some of which were previously considered sterile. Notably, milk is now considered to host a complex microbial community with great diversity. Milk microbiota is now well documented in various hosts. Based on the growing literature on this microbial community, we address here the question of what milk microbiota is. We summarize and compare the microbial composition of milk in humans and in ruminants and address the existence of a putative core milk microbiota. We discuss the factors that contribute to shape the milk microbiota or affect its composition, including host and environmental factors as well as methodological factors, such as the sampling and sequencing techniques, which likely introduce distortion in milk microbiota analysis. The roles that milk microbiota are likely to play in the mother and offspring physiology and health are presented together with recent data on the hypothesis of an enteromammary pathway. At last, this fascinating field raises a series of questions, which are listed and commented here and which open new research avenues.

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Section: Can We Manipulate the Milk Microbiota In Order To Improve Mamentioning

confidence: 99%

Section: Can We Manipulate the Milk Microbiota In Order To Improve Mamentioning

confidence: 99%

Milk Microbiota: What Are We Exactly Talking About?

et al. 2020

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“…Lactic acid bacteria (LAB) is also shown as potential probiotics for therapeutic use against endometrial inflammation and mastitis and prevention for diarrhea in calf, which is capable for the ability to strengthen systemic immune function (Genis et al 2017;Bouchard et al 2015;Maldonado et al 2017). Intramammary LAB incubation increased the amounts of IgG isotypes in blood and milk, as well as lymphocyte proliferation and negative the major bovine mastitis pathogens on dry-off cows, which suggests restored balance in microbiota of the mammary gland and improved systemic immune function (Pellegrino et al 2017). However, the effects of dietary supplementation of LAB and yeast on mastitis and milk microorganisms of lactating cow is unknown.…”

Section: Introductionmentioning

confidence: 99%

Impact of yeast and lactic acid bacteria on mastitis and milk microbiota composition of dairy cows

et al. 2020

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This experiment was conducted to evaluate the impact of yeast and lactic acid bacteria (LAB) on mastitis and milk microbiota composition of dairy cows. Thirty lactating Holstein cows with similar parity, days in milk were randomly assigned to five treatments, including: (1) Health cows with milk SCC < 500,000 cells/mL, no clinical signs of mastitis were found, fed basal total mixed ration (TMR) without supplementation (H); (2) Mastitis cows with milk SCC > 500,000 cells/mL, fed basal TMR without supplementation (M); (3) Mastitis cows fed basal TMR supplemented with 8 g day −1 yeast (M + Y); (4) Mastitis cows fed basal TMR supplemented with 8 g day −1 LAB (M + L); (5) Mastitis cows (milk SCC > 500,000 cells/mL) fed basal TMR supplemented with 4 g day −1 yeast and 4 g day −1 LAB (M + Y + L). Blood and milk sample were collected at day 0, day 20 and day 40. The results showed efficacy of probiotic: On day 20 and day 40, milk SCC in H, M + Y, M + L, M + Y + L was significantly lower than that of M (P < 0.05). Milk concentration of TNF-α, IL-6 and IL-1β in M + Y + L were significantly reduced compared with that of M on day 40 (P < 0.05). Milk Myeloperoxidase (MPO) and N-Acetyl-β-d-Glucosaminidase (NAG) activity of M + Y, M + L, M + L + Y were lower than that of M on day 40 (P < 0.05). At genus level, Staphylococcus, Chryseobacterium and Lactococcus were dominant. Supplementation of LAB decreased abundance of Enterococcus and Streptococcus, identified as mastitis-causing pathogen. The results suggested the potential of LAB to prevent mastitis by relieving mammary gland inflammation and regulating milk microorganisms.

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“…Other constraint are milk disposal owing to its residual effect of antibiotic on the end consumers, worldwide concern for organic food production and ethical considerations of sufferings of animals has limited the antibiotic therapy. Study has been attempted with bovine mastitis prevention by inoculation of lactic acid bacteria at the dry off period ( Pellegrino et al, 2017). Thus host immune response and its immunomodulation may be considered as an alternative for disease resistance.…”

Section: Introductionmentioning

confidence: 99%

Mutations in CD14 gene may have significant effect on mastitis resistance/susceptibility of buffalo.

Pal

Sharma

et al. 2020

Preprint

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Background: CD14 is an important pattern recognition receptor having innate immune function and has antibacterial activity. It binds with LPS of gram-negative bacteria, arachidonic acid, and lipoteichoic acid. Being a receptor, it binds with the pathogen with the help of other cytokines. Mutations in CD14 affect the binding ability which in turn affects the biological potentiality. Method: The present study was conducted on 228 nos. of buffaloes pertaining to four different breeds as Murrah, Mehsana, Surti and Bhadawari. CD14 gene was characterized and polymorphism was detected through Single nucleotide conformation polymorphism. Association study was conducted for different variants of CD14 with mastitis in buffalo, detected through somatic cell count, california mastitis test. Result: Eight variants of CD14 were detected and mutational hotspots were detected in bubaline CD14 with 58 number of non-synonymous SNP, out of which 18 were observed to be deleterious and 34 as thermodynamically unstable. In the present study, we had detected the mutations in CD14 gene and its association with the somatic cell score and other indicators for mastitis. In-silico studies were conducted to understand the molecular mechanism how the mutations affect the biological potentiality by analyzing different domains and structural analysis along with various post-translational modification sites. Conclusion: Deleterious mutations were observed in CD14 gene which have significant effect on mastitis of buffalo. It may be employed for marker assisted selection, therapeutic application of recombinant CD14, gene therapy, transgenic animal production with wild type CD14 resistant to mastitis as future strategy. Keywords: CD14, cytokine, bio-informatics, mutation, SNP, deleterious, I-mutant, Provean, mastitis

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Bovine mastitis prevention: humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period

Cited by 45 publications

References 24 publications

Milk Microbiota: What Are We Exactly Talking About?

Milk Microbiota: What Are We Exactly Talking About?

Impact of yeast and lactic acid bacteria on mastitis and milk microbiota composition of dairy cows

Mutations in CD14 gene may have significant effect on mastitis resistance/susceptibility of buffalo.

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