Identification of SSR markers associated with seed coat permeability and electrolyte leaching in soybean

Abstract

Seed coat permeability and electrolyte leaching are the important traits that have been negatively associated with seed longevity in soybean. The objective of this study was to use SSR markers to identify genomic regions significantly associated with QTLs controlling seed coat permeability and electrolyte leaching in a segregating F 2 population derived from a cross of Birsa soya-1 x JS 71-05. Parental polymorphism survey using 145 SSR markers identified 21 polymorphic ones, which were used to genotype 153 F 2 individuals. Four independent markers (Satt434, Satt538, Satt281 and Satt598) were significantly (P=0.05) associated with seed coat permeability. One of these markers (Satt 281) also showed significant association with electrolyte leaching that partly supported the observed positive correlation (r = 0.425) between the two traits. Markers for seed coat permeability individually explained 3.9% to 4.5% of the total phenotypic variation, while the marker linked with electrolyte leaching explained 5

Genetic studies of soybean [Glycine max (L.) Merr.] response to seed storage stress factors

“…SSR primers that were specifically associated with tolerance alleles (Satt 600) and susceptible alleles (Satt 285, Satt 281) identified were considered as good candidate markers for screening soybean germplasm for the identification of tolerance and susceptible genotypes in soybean breeding programmes. The results corroborates the work of Jagadish et al (2013), Singh et al (2008) and Dargahi et al (2014) that identified same SSR markers to have linkage with storability traits in soybean. Hence, these markers are considered good candidate molecular markers for identifying alleles linked with tolerance or susceptibility to storage stress in soybean.…”

Mapping QTL for important seed traits in an interspecific F2 population of pigeonpea

“…Negative correlation is reported between seed hardness with 100-seed weight and rate of water uptake in soybean (Hirota et al 2005). Significant MTAs have been reported for seed coat permeability (assessed by rate of water uptake) in other legume crops; such as Singh et al (2008) identified four QTLs associated with seed coat permeability in soybean, of which one (satt281) showed association with EC.…”

“…Niu et al (2013) have reported that 100-seed weight is positively influenced by seed size, which is a function of its length, width and thickness. Rate of water uptake by seed is also known to influence seed quality in chickpea (Lamichaney et al 2016), soybean (Singh et al 2008), flax (Saeidi 2008) fababean (Peksen 2007) and cowpea (Peksen et al 2004), which is attributed to embryo damage and subsequently leaching out of electrolytes necessary for the growth and development of embryo. Entry of water into seed during imbibition is regulated by the physical and chemical constituents of seed coat (Lamichaney et al 2017).…”

Genetic Aspects and Molecular Causes of Seed Longevity in Plants—A Review

“…Four ( Satt538 , Satt600 , Satt434 and Satt285 ) independent SSR markers were significantly associated with seed longevity at a distance of 158.63 cM, 75.4 cM, 105 cM and 25.51 cM on chromosomes A2, D1b, H and J, respectively [ 141 ]. The same population was also subjected to seed coat permeability and electrolyte leaching by the same group [ 142 ], where four SSRs ( Satt434 , Satt538 , Satt281 , and Satt598 on chromosomes H, A2, C2, and E, respectively) were associated with seed coat permeability. In addition, SSR Satt281 has been linked to electrolyte leaching on chromosome C2.…”