Identification of spermatozoa using a novel 3-plex MSRE-PCR assay for forensic examination of sexual assaults

Liu, Kuo Lan; Tsai, Li Chin; Lin, Yu Chih; Huang, Nu En; Yang, Lih Jing; Su, Chih Wen; Lee, James Chun-I; Linacre, Adrian; Hsieh, Hsing Mei

doi:10.1007/s00414-020-02285-1

Cited by 5 publications

(3 citation statements)

References 38 publications

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“…The advantage of this approach was the ability to co-amplify STRs at a similar level of sensitivity, but drawbacks included incomplete restriction and template degradation, which can distort the methylation ratios that are critical to the interpretation of the results, which was particularly pronounced in low template samples. More recent assays [155], therefore, use amplicons with multiple restriction sites.…”

Section: Techniques To Analyse Epigenetic Modificationsmentioning

confidence: 99%

“…Since the presence of semen is questioned, most often this DE method will suffice and produce a sperm fraction from which a DNA profile informative for the semen donor is derived. When confirmation of the presence of spermatozoa in the sperm fraction is needed, various methods can be applied such as microscopic analysis or MSRE analysis [154,155,210,211] on DNA extracted from the sperm fraction [212] This is different in circumstance (3), where semen may be present in such amounts that it masks the presence of other body fluids. Here, it would be useful to apply a DE method that not only extracts DNA but also RNA and analyze the non-sperm RNA fraction for cell types other than spermatozoa (note that seminal fluid RNA will end up in this non-sperm RNA fraction as well).…”

Section: Cell Types Questioned In Forensic Casesmentioning

confidence: 99%

“…DNA-based markers (those assessing methylation status) will at best be as sensitive as DNA profiling as both analyze features of the two genomic DNA copies. MSRE analysis [154,155,210,211] indeed approaches the sensitivity of DNA profiling, but methods that rely on a bisulfite conversion are much less sensitive as a large portion of DNA is lost during the conversion [146,147].…”

Section: Sensitivity Specificity and Degradation Issues Affecting Interpretationmentioning

confidence: 99%

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On the Identification of Body Fluids and Tissues: A Crucial Link in the Investigation and Solution of Crime

Sijen

Harbison

2021

Genes

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Body fluid and body tissue identification are important in forensic science as they can provide key evidence in a criminal investigation and may assist the court in reaching conclusions. Establishing a link between identifying the fluid or tissue and the DNA profile adds further weight to this evidence. Many forensic laboratories retain techniques for the identification of biological fluids that have been widely used for some time. More recently, many different biomarkers and technologies have been proposed for identification of body fluids and tissues of forensic relevance some of which are now used in forensic casework. Here, we summarize the role of body fluid/ tissue identification in the evaluation of forensic evidence, describe how such evidence is detected at the crime scene and in the laboratory, elaborate different technologies available to do this, and reflect real life experiences. We explain how, by including this information, crucial links can be made to aid in the investigation and solution of crime.

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Section: Techniques To Analyse Epigenetic Modificationsmentioning

confidence: 99%