A novel co-amplification system for simultaneous amplification of 23 Y-STR and identification of spermatozoa

Lin, Yu-Chih; Tsai, Li-Chin; Liu, Kuo-Lan; Huang, Nianyu; Yang, Lih-Jing; Su, Chung‐Kuang; Lee, James Chun-I; Linacre, Adrian; Hsieh, Hsing-Mei

doi:10.1007/s00414-021-02723-8

Cited by 1 publication

(2 citation statements)

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“…In general, the Y-semen markers strengthen the proof of semen even when autosomal markers provide no clear results. Lin et al reported detection of their autosomal semen marker in the 3-plex assay with ratios of 1:50 for blood and even 1:800 for vaginal secretion [ 8 ]. Interestingly, with the 10-plex assay containing two autosomal semen markers they could detect semen with ratios only up to 1:10 [ 7 ].…”

Section: Discussionmentioning

confidence: 99%

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Expanding the scope of methylation-sensitive restriction enzyme (MSRE) PCR for forensic identification of body fluids through the novel use of methylation-dependent restriction enzymes (MDRE) and the combination of autosomal and Y-chromosomal markers

Rothe,

Becker,

Charchinezhadamouei

et al. 2023

Int J Legal Med

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Methylation-sensitive/-dependent restriction enzyme (MSRE/MDRE) PCR can be performed to detect hypomethylated or hypermethylated CpG sites. With the combined use of different tissue-specific CpG markers, MSRE/MDRE-PCR leads to tissue-specific methylation patterns (TSMPs), enabling the correlation of DNA samples to their source tissue. MSRE/MDRE assays can use the same platform as forensic STR typing and offer many advantages in the field of forensic body fluid detection. In the present study, we aimed to establish MSRE assays for the detection of blood, saliva, vaginal secretion, and semen, using markers from literature and from our own database search. We designed two different MSRE test-sets, which include two novel Y-chromosomal non-semen markers, and enable differentiation between female and male non-semen samples. Furthermore, we established an MSRE/MDRE semen approach, which includes only Y-chromosomal non-semen and semen markers. This Y-semen multiplex PCR utilizes the novel combination of the methylation-sensitive enzyme SmaI and the methylation-dependent enzyme GlaI, which enables more sensitive detection of male body fluids within male/female DNA mixtures. Our validation tests confirmed that MSRE/MDRE assays exhibit high sensitivity, similar to that of STR typing.

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Section: Discussionmentioning

confidence: 99%

“…Although MSRE-PCR offers several advantages, little research has been performed to test this method. Notably, Lin and colleagues have discovered new markers for MSRE-PCR, and have impressively demonstrated the simultaneous amplification of MSRE bf and STR markers [ 7 , 8 ]. To date, only a few markers for MSRE-PCR have been identified.…”

Section: Introductionmentioning

confidence: 99%