Identification of specific proteins in colorectal cancer patients with Schistosoma mansoni infection as a possible biomarker for the treatment of this infection

Abdelkareem, Eshtiyag Abdalla; Cheong, Tan Gim; Sharief, Abdalla Hassan; Lim, Boon Huat; Khoo, Boon Yin

doi:10.1016/s2222-1808(14)60714-3

Cited by 6 publications

(2 citation statements)

References 36 publications

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“…IL-21 was the focus of the present study, as a high level of IL-21 expression has been detected in colorectal cancer cells, such as HT29 and HCT116( 7 ). In our previous study, a high level of IL-21 was also detected in the serum samples of patients diagnosed with colorectal cancer with a history of parasitic infections ( 24 ). IL-18 or IL-21 alone have been demonstrated to represent weak inducers of IFN-γ production, but the combination of IL-18 and IL-21 has been reported to induce notable activation of IFN-γ gene expression ( 19 ).…”

Section: Discussionmentioning

confidence: 86%

IFN‑γ and IL‑18 in conditioned media of parasite‑infected host and IL‑21‑silenced colorectal cancer cells

Ong

Abdalkareem

et al. 2020

Exp Ther Med

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The presence of certain soluble factors may provide a possible selective advantage for a parasite to gradually modify cell proliferation in neighbouring cells, which may result in chronic diseases. These soluble factors present in the conditioned medium also allow the parasite to invade rapidly into more host cells. The present study aimed to determine the levels of a group of type 1 T helper (Th1) cytokines in the conditioned media of host cells infected with parasites and in IL-21-silenced colorectal cancer cells. The conditioned media of human foreskin fibroblasts (HFFs) parasitized with the RH and ME49 strains of Toxoplasma gondii for 10 days were prepared, and subsequently the levels of the Th1 cytokines in the conditioned media were determined by ELISA. HFFs were incubated with the growth media containing selected soluble factors, and cell proliferation markers were subsequently analysed by reverse transcription-quantitative PCR. The mRNA expression level of cell proliferation markers was also examined in IL-21-silenced HCT116 cells, where the levels of soluble factors in the conditioned media were also determined as aforementioned. The results of the present study demonstrated that HFFs parasitized with ME49 released elevated levels of IFN-γ and lower levels of IL-18 into the conditioned medium compared with the controls. These phenomena were not observed in the conditioned medium of HFFs parasitized with RH. Similar levels of these soluble factors were also detected in the conditioned medium of IL-21-silenced HCT116 cells. The results of the present study also revealed that Ki67 and proliferating cell nuclear antigen mRNA expression was altered in host cells incubated with various levels of IFN-γ and IL-18, as well as in IL-21-silenced HCT116 cells compared with the respective controls. In conclusion, the current study provided preliminary evidence on the fundamental molecular mechanisms of host-parasite interactions that result in chronic diseases, which may aid in the treatment of these diseases in the relevant endemic regions.

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Section: Discussionmentioning

confidence: 86%

IFN‑γ and IL‑18 in conditioned media of parasite‑infected host and IL‑21‑silenced colorectal cancer cells

Ong

Abdalkareem

et al. 2020

Exp Ther Med

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“…Another study that recruited 93 patients with colorectal cancer with or without S. mansoni infection in Sudan reported that 40-43% of the patients diagnosed with malignant colorectal cancer by colonoscopy and histopathology were infected with S. mansoni [20]. Furthermore, 40% of the colorectal cancer patients infected with S. mansoni were younger than 40 years [21,22] and as much as 21% of young children were infected with S. mansoni in the country [23,24].…”

Section: Epidemiological Evidencementioning

confidence: 99%

A Current Perspective of Schistosomiasis in Association with Colorectal Carcinogenesis

Abdalkareem¹,

Yin²

2019

TOIDJ

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Background: Schistosomiasis is one of the parasitic infections that are often found in humans. More than approximately 200 million people are infected with Schistosomiasis in tropical and subtropical areas of Africa, South America and Asian countries. Literature has long been suggesting the correlation between Schistosomiasis and colorectal malignancy. There is a considerable directory supporting the etiological relation between Schistosoma japonicum infection and colorectal cancer in the Far East, however, the available data about the role of Schistosoma mansoni that can initiate the carcinogenesis of colorectal remain insignificant. Objective: As such, more studies of this disease should be conducted comprehensively for corporate social responsibility internationally. Methods: The present study reviewed the available data about the role of Schistosoma, including S. mansoni in association with the carcinogenesis of colorectal. Results: The study shows the possible evidence of epidemiology, pathology, molecules and immunopathology associated with Schistosomal infections and colorectal cancer. The infections are apparently getting little attention nor support worldwide due to the geographical barriers and some political issues because it mainly occurs in the people living in the bottom billion and happens in the endemic regions only. Conclusion: The in-depth study of this infectious disease will tailor early diagnosis, novel prescription drugs and cost-effective strategies for the treatment of infectious disease colorectal cancer, and hence eradicate the disease in the endemic regions.

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From the discovery of helminths to the discovery of their carcinogenic potential

Jain,

Rana

2023

Parasitol Res

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Identification of specific proteins in colorectal cancer patients with Schistosoma mansoni infection as a possible biomarker for the treatment of this infection

Cited by 6 publications

References 36 publications

IFN‑γ and IL‑18 in conditioned media of parasite‑infected host and IL‑21‑silenced colorectal cancer cells

IFN‑γ and IL‑18 in conditioned media of parasite‑infected host and IL‑21‑silenced colorectal cancer cells

A Current Perspective of Schistosomiasis in Association with Colorectal Carcinogenesis

From the discovery of helminths to the discovery of their carcinogenic potential

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