Identification of specific binding sites for nerve growth factor on human blood platelets and membranes from bovine brain

Abstract

The presence of specific binding sites for nerve growth factor on membranes from bovine brain and human blood platelets is shown. The association and dissociation kinetics and the dissociation constants of receptor binding of nerve growth factor are analyzed.

Platelet-derived nerve growth factor supports the survival of cholinergic neurons in organotypic rat brain slices

“…two neurotrophins: NGF and brain-derived neurotrophic factor (BDNF). The role of NGF in platelets is not well-investigated, but it has been shown that NGF can induce platelet aggregation [2]. We have recently shown that NGF is spontaneously released upon activation within minutes, and different to the release of BDNF [3].…”

Application of Mathematical Modeling to Analysis of Nerves Growth Factor Interaction with Platelets

“…The blood volume was 15 ml. Platelets from total blood were selected by the gel-filtration method (6 cells/ml. The total volume of the incubation solution was 1 ml.…”

“…Earlier we had showed the impossibility of applying Scatchard analysis to the definition of NGF receptor binding parameters on human peripheral blood platelets, in some cases due to nonlinearity Scatchard plots (6). Therefore, the purpose of the present work was to research the possibility of applying mathematical modeling to the analysis of the interaction of NGF platelets.…”

“…NGF functions on target cells are initiated by the interaction of NGF with two types of specific receptors: low and high affinity (dissociation constants are on the order of 10 −9 and 10 −11 , respectively) (1,2). The NGF receptors are circumscribed on Schwann cells (3), on keratinocytes (4), on lymphocytes (5), and on human platelets (6 ).…”

Depression and Cardiovascular Disease: The Viewpoint of Platelets

“…The impact of NTs on platelet function has not been well-investigated yet. To our knowledge, it is only known that NGF can bind platelet surface and induce aggregation [ 214 ], while no information is available on the effect of BDNF on platelet function, even though BDNF can bind a specific site on platelets surface with subsequent internalization [ 215 ]. Platelets contain both NGF and BDNF, that are spontaneously released upon platelet activation [ 215 , 216 , 217 , 218 , 219 ].…”