Application of Mathematical Modeling to Analysis of Nerves Growth Factor Interaction with Platelets

Gurevich, K. G.; Chekalina, N. D.; Zakharenko, O.M.

doi:10.1006/bioo.1999.1153

Cited by 2 publications

(2 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To address it, we conducted a fluorescence titration using FACS (Figure S3) to compare the binding affinity of the dimeric G4PA and monomeric control (Figure e), of which the Scatchard plot shows a TfR is attached by two TfR aptamers with a synergistic effect reflected by the downward concavity (Figure S4). − The dimerization of G4PA holds the two aptamers close, and such a proximity effect contributes to their effective corporation in binding TfR, just as other proximity-enhanced aptamer–ligand interactions. − …”

mentioning

confidence: 99%

G-Quadruplex-Proximized Aptamers (G4PA) Efficiently Targeting Cell-Surface Transferrin Receptors for Targeted Cargo Delivery

Wang

Shi

et al. 2022

Nano Lett.

View full text Add to dashboard Cite

DNA-assembled multiaptamer systems have been demonstrated to significantly promote the aptamer capacity of binding cell-surface-expressed proteins. However, how to conveniently harness them for efficient transmembrane delivery of targets remains a challenge. Toward this goal, here we engineer a G-quadruplex-proximized aptamer (G4PA) system in which a DNA aptamer specific for transferrin receptor (TfR) is guided by a bimolecular G4 and assembles into a dimerized proximity form that well matches homodimeric TfR highly expressed on the cancer cell surface. This system displays a higher capacity for targeting cell-surface TfR than the monomeric aptamer and super transmembrane transportation of nucleic acid cargoes, which is comparable to that of conventional liposome transfection but overcomes the lack of targeting ability of the latter. The G4PA system is then applied to the targeted delivery of siRNA for PLK1 gene silencing in positive cells rather than negative controls, showing great promise for use in precise anticancer therapy.

show abstract

mentioning

confidence: 99%

G-Quadruplex-Proximized Aptamers (G4PA) Efficiently Targeting Cell-Surface Transferrin Receptors for Targeted Cargo Delivery

Wang

Shi

et al. 2022

Nano Lett.

View full text Add to dashboard Cite

show abstract

“…There have been extensive studies on the biological and physiological effect of platelet aggregations. Some mathematical models and computational models are very instructive for the modelling of the detailed mechanism of the formation of platelet aggregates [3][4][5][6]. Most of these studies use the Navier-Stokes equations with simplified boundary conditions or the steady-state approximation.…”

Section: Introductionmentioning

confidence: 99%

Pulsating Flow and Platelet Aggregation

Yang

2005

Lecture Notes in Computer Science

View full text Add to dashboard Cite

Platelet aggregation has been modelled as a nonlinear system of viscous flow and pulsating flow based on the Fogelson's continuum model. By solving nonlinear governing equations and the coupling between flow and platelet aggregates in the cylindrical coordinates using the finite element method, we can see how the platelet aggregation forms under the pulsating flow conditions. Numerical simulations show that the aggregates are stretched by the flow in the dynamic equilibrium of the link forces and background flow. In addition, significant elastic stress can be developed to maintain the shape and integrity of the platelet aggregates.

show abstract

Application of Mathematical Modeling to Analysis of Nerves Growth Factor Interaction with Platelets

Cited by 2 publications

References 8 publications

G-Quadruplex-Proximized Aptamers (G4PA) Efficiently Targeting Cell-Surface Transferrin Receptors for Targeted Cargo Delivery

G-Quadruplex-Proximized Aptamers (G4PA) Efficiently Targeting Cell-Surface Transferrin Receptors for Targeted Cargo Delivery

Pulsating Flow and Platelet Aggregation

Contact Info

Product

Resources

About