Identification of Species-Diagnostic ISSR Markers for Six Eucalyptus Species

Abstract

Eucalyptus is planted worldwide for raw material in paper and rayon industry. It is a potential out-crosser and the natural populations are highly heterogeneous displaying strong inbreeding depression. Eucalyptus hybrids have been intensively utilized for their vigor, higher wood quality and resistance to diseases. Identification of species for hybridization is predominantly based on morphological characters and is not always reliable. Hence, DNA marker based species identification and hybrid validation is an important and efficient tool in breeding programs. In the present study, attempts were made to identify species - diagnostic markers for six eucalypt species (E. camaldulensis Dehnh, E. citriodora Hook, E. grandis W. Hill ex Maiden, E. pellita F. Muell, E. tereticornis Sm and E. urophylla S.T. Blake) using ISSR-PCR fingerprints. PCR amplification using seven ISSR primers resulted in significant polymorphism among the population from different species. E. citriodora and E. tereticornis showed monomorphic frequency of maximum 37.5% and minimum 14.3% respectively. Twenty species-diagnostic markers were identified for E. camaldulensis, E. citriodora, E. grandis and E. urophylla while no marker was detected for E. pellita and E. tereticornis. A maximum of eleven and a minimum of one species-diagnostic marker were recorded for E. citriodora and E. camaldulensis respectively. Among the twenty markers, nine were present in all the individuals of a particular species.

Identifi cation of Species-Diagnostic Inter Simple Sequence Repeat Markers for Ten Phyllanthus Species

“…Species-diagnostic ISSR markers were identifi ed in P. acidus, P. emblica, P. fraternus, P. urinaria, P. rotundifolius, P. amarus, and P. angustifolius while no diagnostic markers were observed in P. reticulatus, P. nivosus, and P. virgulatus. A similar problem was experienced while developing species-specifi c markers using RAPDs (Scheef et al, 2003) and ISSRs (Balasaravanan et al, 2006). This could be due to the occurrence of high divergence among the particular species populations.…”

“…Isolation of specifi c diagnostic markers for particular taxa could be of great use in proper identifi cation of these taxa. However, only a few reports on species-specifi c markers in plants are available (Scheef et al, 2003;Kochieva, 1999;Kochieva et al, 2004;Balasaravanan et al, 2006;Theerakulpisut et al, 2008;Feng et al, 2010).…”

“…In another study, Kochieva et al (2004) revealed both species-and cultivar-specifi c markers in the genus Syringa. Balasaravanan et al (2006) identifi ed eight species-specifi c diagnostic markers for six species of Eucylaptus. Feng et al (2010) studied the internal transcribed spacer (ITS) regions of Angelica sinensis and seven other Angelica species used as adulterants for authentication of A. sinensis.…”

Preliminary genetic approach based on both cytogenetic and molecular characterisations of nine oak species

“…ISSR markers already proved to be a powerful tool for DNA fingerprinting in Eucalyptus (Balasaravanan et al 2006), Populus (Gao et al 2006) and other tree species (see Carvalho et al 2005).…”

Development of SCAR marker in Casuarina equisetifolia for species authentication

“…PCR-based DNA markers are now used by systematists and germplasm managers for species/cultivar identification and validation in trees as reported in eucalypts (Grattapaglia et al 1992;Balasaravanan et al 2006), spruce (Khasa and Dancik 1996), Morus spp. (Tani et al 2003), olive (Bautista et al 2003) and Larix sp.…”