Identification of somatic embryogenesis (SE) related proteins through label-free shotgun proteomic method and cellular role in Catharanthus roseus (L.) G. Don

Gulzar, Basit; Mujib, A.; Rajam, Manchikatla Venkat; Frukh, Arajmand; Zafar, Nadia

doi:10.1007/s11240-019-01563-0

Cited by 18 publications

(6 citation statements)

References 34 publications

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“…Here, Kinetin alone and in combination with 2,4-D was noted to be more efficient for embryo development and advancement. Several other researchers demonstrated auxins specifically 2,4-D’s efficacy (as most significant and strong PGR) in initiating somatic embryos in different plant species (Zhang et al 2007 ; Gulzar et al 2019 ). Since Daucus carrota, the incidence of somatic embryogenesis has been reported widely and histological and SEM evidence has been presented in many plant species (Couillerot et al 2012 ; Mujib et al 2013 ).…”

Section: Discussionmentioning

confidence: 99%

“…Somatic embryogenesis is a sequential process where somatic cells acquire cellular totipotency under an appropriate culture environment and induce embryogenic cells and embryos (Feher 2015 , Gulzar et al 2019 ). The plant produced via callogenesis shows the stages of callus formation, acquisition of embryogenic cells, embryo formation, maturation and plantlet regeneration (Von Arnold et al 2002 ).…”

Section: Discussionmentioning

confidence: 99%

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Flow cytometry and start codon targeted (SCoT) genetic fidelity assessment of regenerated plantlets in Tylophora indica (Burm.f.) Merrill

Mamgain¹,

Mujib²,

Ejaz³

et al. 2022

Plant Cell Tiss Organ Cult

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Tylophora indica (Burm.f.) Merrill. is a pharmacologically important plant, popular for alkaloidal and non-alkaloidal richness. Large scale propagation of T. indica is difficult in the wild as the seeds are small and the frequency of germination is very poor. In the present study, the genome size estimation of in vitro regenerated (indirect, direct and somatic embryo mediated) T. indica was made by flow cytometric method. Clonal fidelity of the regenerants was assessed using a start codon targeted (SCoT) molecular marker. Initially, the explants were inoculated on Murashige and Skoog basal medium supplemented with various concentrations of plant growth regulators like 2,4-dichlorophenoxy acetic acid (2,4-D), Kinetin, 6-benzyl amino purine (BAP) and 1-naphthalene acetic acid either singly or in combinations. The highest callus induction frequency (87.75%) was obtained in 6.7 µM 2,4-D added MS medium which metamorphosed into progressive stages (globular, heart, torpedo, and cotyledonary) of embryos. Mature and healthy somatic embryos efficiently germinated into plantlets on 8.8 µM BAP + 1.4 µM GA 3 enriched MS medium. Histological and scanning electron microscopic study confirmed the above developing stages . The regenerated shoots were rooted best in 2.45 µM Indole-3-butyric acid supplemented solid MS medium. The plants were hardened and acclimatized with 90% survivability. The flow cytometric 2C DNA content of indirect, direct and somatic embryo derived plants was 1.896 pg, 1.940 pg and 1.926 pg respectively, very similar to the mother plant (1.928 pg). SCoT marker generated a high percentage of monomorphic bands (94%) revealing similarity with the mother plant, thus ensuring genetic fidelity. To the best of our knowledge, this is perhaps the first ever report of 2C DNA content estimation and SCoT marker based genetic homogeneity study in T. indica . Supplementary Information The online version contains supplementary material available at 10.1007/s11240-022-02254-z.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Flow cytometry and start codon targeted (SCoT) genetic fidelity assessment of regenerated plantlets in Tylophora indica (Burm.f.) Merrill

Mamgain¹,

Mujib²,

Ejaz³

et al. 2022

Plant Cell Tiss Organ Cult

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“…In this study, the lycorine content of bulb, leaf, and root of field grown and tissue culture regenerated plants was measured in three different species of Zephyranthes; and the impact of MJ elicitation on lycorine yield was investigated in in vitro derived plant parts. For successful tissue culture propagation, the sterilization steps, the genotype, the selection of explants, explant orientation, PGR concentration, and combinations are considered critical [35,36]. In bulbous monocotyledonous plants, the establishment of callus and rapid synchronous somatic embryogenesis are reported difficult thus the multiplication of organs like bulblets is suggested to be an efficient alternative way for mass propagation of plants and production of bioactive compounds.…”

Section: Discussionmentioning

confidence: 99%

“…The total soluble protein was also increased in response to MJ. The increase in protein during stress is considered to be one of the stress adapting strategies of plants to prevent oxidative damage to cells [36].…”

Section: Discussionmentioning

confidence: 99%

Methyl Jasmonate Elicitation for In Vitro Lycorine Accumulation in Three Zephyranthes Species and Comparative Analysis of Tissue-Cultured and Field Grown Plants

Syeed,

Mujib,

Dewir

et al. 2023

Horticulturae

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Lycorine is an important Amaryllidaceae alkaloid showing anti-cancerous activity on numerous cell lines; and it also demonstrates antiviral activity against several viruses including SARS-CoV-2 responsible for COVID-19. The in vitro cultivation of plant parts is a good alternative for elicitor mediated secondary metabolite synthesis, mass propagation, and cloning of elite genotypes. In this study, the quantification of lycorine from Zephyranthes candida, Zephyranthes grandiflora, and Zephyranthes citrina was evaluated, and the influence of methyl jasmonate (MJ) treatments on accumulation of lycorine yield was observed in the in vitro cultivated tissues. The influence of methyl jasmonate (MJ) on accumulation of lycorine was investigated for the first time in in vitro regenerated bulb, leaf, and root tissues of Zephyranthues. The efficient in vitro shoot regeneration was achieved in Z. candida (73.9%), Z. grandiflora (85.5%), and Z. citrina (76.5%) on Murashige and Skoog (MS) medium amended with 2.0 mg·L−1 Naphthalene acetic acid + 0.5 mg·L−1 Benzylaminopurine. The regenerants had the highest i.e., 7.46 mean numbers of bulblets per shoot. The best root induction (80.66%) was achieved on 2.0 mg/L Indole butyric acid (IBA). The lycorine content was quantified through High performance thin layer chromatography (HPTLC) in three field grown Zephyranthes species, was highest in Z. candida (1.93 µg g−1 dry wt.), followed by Z. grandiflora (1.87 µg g−1 dry wt.) and Z. citrina (1.62 µg g−1 dry wt.). The yield of lycorine was observed in this order i.e., bulb > leaf > root. The regenerated plants were supplemented with MJ and maximum lycorine yield was noted at 100 µM in bulb tissues of Z. candida (2.74 µg g−1 dry wt.) with an increase in percentage of 39.08. The enhancement could be due to MJ-induced stress as the biochemical attributes and anti-oxidant enzyme activity were high with elevated level of MJ. This enrichment may auger commercial manufacturing and utilization of lycorine in future.

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“…Somatic embryogenesis is the process to form a whole plant from a single somatic cell (El-Sherif, 2019) throughout a series if of morphological and biochemical changes (Gulzar, Mujib, Rajam, Frukh, & Zafar, 2019;Nic-Can et al, 2015). Initially, regeneration of citrus via somatic embryogenesis was possible in liquid cultures (Agisimanto et al, 2012;Altaf, Khan, Sadia, Jaskani, & Khan, 2017;Februyani et al, 2016;Gerolino et al, 2015;Puad, Sarji, Fathil, & Abduh, 2018), and the process was usually influenced by several factors, both in solid and liquid culture (Widoretno et al, 2017).…”

Section: Aeration Rate Effect On Somatic Embryogenesis In An Air-liftmentioning

confidence: 99%

Rapid Somatic Embryogenesis of Citrus reticulata Blanco cv. Madu in an Air-Lift Bioreactor Culture

Agisimanto¹,

Normah

Ibrahim

2019

Agrivita.J.Agr.Sci

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Embryogenic cell (EC) growth and proliferation as well as somatic embryo induction were examined in a bioreactor culture using Murashige and Skoog basal medium particularly for the influence of 6-benzyladenine (BA) concentration, initial cell density and aeration rate. Embryogenic callus was induced from seeds of Citrus reticulata Blanco cv. Madu. The cell suspension in a 3-L bioreactor exhibited maximum cell growth following the addition of 1.5 mg/l of BA. The fresh weight (FW) of the cells after 28 days of growth was found to increase from an initial of 5.5 g cell culture to 57.3 g, a 10.4-fold increase and the maximum growth rate (GR) of the cells (0.33 g/day) was attained by the 7 th day of culture. In the cell density experiment, ECs at a concentration of 5.5 g/l constituted the most effective inoculum, reaching the highest GR of ECs (0.52 g/ day), again by the 7 th day of culture. In the aeration experiment, the highest EC GR of 2.6 g/day was obtained at the maximum aeration rate of 1.5 vvm (air volume medium/ volume/min). After 28 days of somatic embryogenesis, 79% of ECs became somatic embryos, of which 29% were at cotyledonary stage.

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Identification of somatic embryogenesis (SE) related proteins through label-free shotgun proteomic method and cellular role in Catharanthus roseus (L.) G. Don

Cited by 18 publications

References 34 publications

Flow cytometry and start codon targeted (SCoT) genetic fidelity assessment of regenerated plantlets in Tylophora indica (Burm.f.) Merrill

Flow cytometry and start codon targeted (SCoT) genetic fidelity assessment of regenerated plantlets in Tylophora indica (Burm.f.) Merrill

Methyl Jasmonate Elicitation for In Vitro Lycorine Accumulation in Three Zephyranthes Species and Comparative Analysis of Tissue-Cultured and Field Grown Plants

Rapid Somatic Embryogenesis of Citrus reticulata Blanco cv. Madu in an Air-Lift Bioreactor Culture

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