Methyl Jasmonate Elicitation for In Vitro Lycorine Accumulation in Three Zephyranthes Species and Comparative Analysis of Tissue-Cultured and Field Grown Plants

Abstract

Lycorine is an important Amaryllidaceae alkaloid showing anti-cancerous activity on numerous cell lines; and it also demonstrates antiviral activity against several viruses including SARS-CoV-2 responsible for COVID-19. The in vitro cultivation of plant parts is a good alternative for elicitor mediated secondary metabolite synthesis, mass propagation, and cloning of elite genotypes. In this study, the quantification of lycorine from Zephyranthes candida, Zephyranthes grandiflora, and Zephyranthes citrina was evaluated, and the influence of methyl jasmonate (MJ) treatments on accumulation of lycorine yield was observed in the in vitro cultivated tissues. The influence of methyl jasmonate (MJ) on accumulation of lycorine was investigated for the first time in in vitro regenerated bulb, leaf, and root tissues of Zephyranthues. The efficient in vitro shoot regeneration was achieved in Z. candida (73.9%), Z. grandiflora (85.5%), and Z. citrina (76.5%) on Murashige and Skoog (MS) medium amended with 2.0 mg·L−1 Naphthalene acetic acid + 0.5 mg·L−1 Benzylaminopurine. The regenerants had the highest i.e., 7.46 mean numbers of bulblets per shoot. The best root induction (80.66%) was achieved on 2.0 mg/L Indole butyric acid (IBA). The lycorine content was quantified through High performance thin layer chromatography (HPTLC) in three field grown Zephyranthes species, was highest in Z. candida (1.93 µg g−1 dry wt.), followed by Z. grandiflora (1.87 µg g−1 dry wt.) and Z. citrina (1.62 µg g−1 dry wt.). The yield of lycorine was observed in this order i.e., bulb > leaf > root. The regenerated plants were supplemented with MJ and maximum lycorine yield was noted at 100 µM in bulb tissues of Z. candida (2.74 µg g−1 dry wt.) with an increase in percentage of 39.08. The enhancement could be due to MJ-induced stress as the biochemical attributes and anti-oxidant enzyme activity were high with elevated level of MJ. This enrichment may auger commercial manufacturing and utilization of lycorine in future.

Identification of Volatile Compounds and Terpene Synthase (TPS) Genes Reveals ZcTPS02 Involved in β-Ocimene Biosynthesis in Zephyranthes candida

“…In addition to its decorative value, Z. candida contains various secondary metabolites, including alkaloids [1], flavonoids, and volatile organic compounds (VOCs), which potentially contribute to its medicinal properties. Traditional medicine practices have employed it for diverse therapeutic purposes because of diuretic, emmenagogue and antirheumatic properties [2]. Research into Z. candida has focused on cultivation management [3], pest control, and chemical compounds [4].…”

Fungal Elicitation Enhances Vincristine and Vinblastine Yield in the Embryogenic Tissues of Catharanthus roseus

“…Elicitors are a large target group of compounds which have been added to media at various stages of cultural growth for improving secondary compounds. Traditionally, an 'elicitor' is a molecule which is introduced into a medium in small levels to improve the biosynthesis of compounds by triggering cellular defense response genes [11,12]. The process also refers to compounds of various sources which stimulate physiological and morphological responses in inducing compounds of a defensive nature [13].…”