Identification of SNPs of the <i>L-BABP </i>and <i>L-FABP </i>and their Association with Growth and Body Composition Traits in Chicken

Abstract

Fatty acid-binding proteins (FABPs) are members of lipid-binding proteins that participate in lipid uptake and intracellular transport. This study was designed to investigate the effects of the liver bile acid-binding protein (L-BABP) and the liver fatty acid binding protein (L-FABP) genes on chicken growth and body composition traits. Three SNPs (SNP1, SNP2 and SNP3) of the L-BABP and six SNPs (SNP7-SNP9, SNP11-SNP13) of the L-FABP were detected firstly by DNA sequencing. Seven SNPs (SNP6 and SNP15-SNP20) of the L-FABP were detected by DNA sequencing and were found in the chicken genomic databases at the same time. Sequenom MassARRAY was developed to genotype 966 birds from the Northeast Agricultural University F 2 (NEAUF 2 ) resource population and the Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF) population. Linkage disequilibrium analysis showed that the L-BABP SNPs appeared to be strongly linked with each other and were located within a haplotype block in both populations. SNP7 and SNP8, SNP12 and SNP13, SNP19 and SNP20 of the L-FABP were in strong linkage disequilibrium, respectively. The L-BABP polymorphisms were significantly associated with body weight (BW) (P＜0.05) in the NEAUF 2 and the NEAUHLF populations and abdominal fat weight (AFW) and abdominal fat percentage (AFP) (P＜0.05) in the NEAUHLF population. The L-BABP diplotypes were significantly associated with BW (P＜0.05) in the NEAUF 2 and the NEAUHLF populations. There was no consistent association between the thirteen SNPs of the L-FABP and the growth and body composition traits in either the NEAUF 2 or the NEAUHLF populations. The research suggests that the L-BABP could be a candidate locus to affect growth traits in chickens.

Association between FTO polymorphism in exon 3 with carcass and meat quality traits in crossbred ducks

“…The three haplotypes that were found to support the results of a previous study by Lee et al (2012), which also reported strong linkage disequilibrium (Zhao et al, 2013). Analysis at the protein level has revealed that the substitution of serine by a phenylalanine in SNP 473 would not affect its secondary and tertiary structures, confirming the lack of a significant association with meat quality traits.…”

“…In commercial pig populations, allele C of the FTO SNP g.400C>G in exon 3 is significantly associated with backfat depth, and allele G is significantly associated with muscle traits and has the greatest effect on thoracic tissues (Dvořáková et al, 2012). The FTO SNPs c.499G>A and c.453C>A in exon 3 are significantly associated with BW at 35, 70, and 84 days of age in New Zealand rabbits (P < 0.01), and the synonymous SNP c.660T>C is significantly associated with BW at 84 days of age, ADG, and the IMF content of the longissimus lumborum in Ira rabbits (P < 0.01) (Zhang et al, 2013). Fontanesi et al (2009) found that the FTO SNP g.276T>G in intron 4 is associated with IMF deposition in Italian Duroc pigs, and the feed: gain ratio in Italian Large White pigs.…”

Enhanced Methanol Oxidation in Acid Media on Pt/S, P Co‐doped Graphene with 3D Porous Network Structure Engineering

“…From Figure a and b, it was observed that Pt nanoparticles are uniformly distributed (generally spherical) and thickly loaded on the Pt/3D‐SPG catalyst, while those in Pt/SPG were seriously aggregated and rarely loaded. This improved distribution and high loading could be attributed to 3D porous network structure in 3D‐SPG, which offer a great deal of anchored sites for the loading of Pt nanoparticles ,. According to Figure c and d, the lattice planes of Pt nanoparticles are distinctly showed with a same spacing (0.227 nm), demonstrating the S and P co‐doped does not change crystal structure of Pt .…”

Role of a liver fatty acid-binding protein gene in lipid metabolism in chicken hepatocytes

“…L-BABP is only expressed in the liver (QingQiu et al, 2011), and it substantially affects chicken body weight, abdominal fat weight, and abdominal fat percentage (Zhao et al, 2013). L-BABP also exhibited a negative correlation with the amount of total CHOL (Gao et al, 2015).…”