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ABSTRACT. The fat mass and obesity-associated gene (FTO) is an excellent candidate gene that affects energy metabolism. Single nucleotide polymorphisms (SNPs) in FTO are associated with carcass and meat quality traits in pigs, cattle, and rabbits. The aim of this study was to investigate the association between novel SNPs in the FTO coding region and carcass and meat quality traits in 95 crossbred ducks, using DNA sequencing. We found two transitions G/A (SNP 387 and 473) within exon 3. SNP 387 was a synonymous mutation, whereas SNP 473 was a missense mutation. Association analysis suggested that SNP g.387G>A was significantly associated with all of the carcass traits measured, the intramuscular fat content (IMF), cooking yield (CY), pH values 45 min after slaughter (pH45m), drip losses from the breast muscle, and the leg muscle (P < 0.05). For SNP g.473G>A, the genotype AA exhibited greater leg muscle weight than the genotypes GG or AG (P < 0.05). The D value suggested that the two SNPs exhibited strong linkage disequilibrium. Three haplotypes (G 1 G 2 , G 1 A 2 , and A 1 A 2 ) were significantly associated with IMF, CY, the a* value, and all of the carcass traits measured (P < 0.05). The results suggest W. Gan et al. 6700©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 6699-6714 (2015) that FTO is a candidate locus that affects carcass and meat quality traits in ducks.

Biological agents are becoming increasingly popular for therapeutic applications in epidermal diseases. Ethosomes facilitate the transdermal ⁄ topical delivery of biological macromolecules. The mouse epidermal growth factor (mEGF) was selected as the model biological agent. The aim of this experiment was to determine the penetration pathways and biological functions of the mEGF ethosomal delivery system after its topical application. The mEGF ethosomal delivery system was topically applied on the dorsal skin of C57BL ⁄ 6 mice at different time points. Freshly excised skin samples were obtained by skin biopsies and shockfrozen, and immunofluorescence was performed. The results showed that penetration of mEGF ethosomes was mainly through the pilosebaceous unit and partly through the intercellular domain. Biological agents encapsulated in the ethosomal delivery system could reach each site of the pilosebaceous unit. We also found that mEGF ethosomes had caused successful transition of the hair follicles from the telogen to the anagen phase of the hair cycle.

This study aimed to investigate the influence of polymorphisms in chemokine genes, including MCP1, CCR2, and CCR5 with psoriasis vulgaris (PV) risk in a Chinese population.The genotyping of studied polymorphisms through polymerase chain reaction (PCR) and sequencing was conducted in 142 PV patients and 147 healthy controls. The genotype distribution of the polymorphisms in the control group was checked to determine whether it conformed to Hardy–Weinberg equilibrium (HWE). The genotype and allele frequencies were compared between PV patients and the healthy controls using Chi-square test. Odds ratio (OR) with 95% confidence interval (95% CI) was calculated to assess the relative risk of PV related to genetic variants.CCR2 rs1799864 polymorphism was associated with significantly elevated risk of PV (AA+AG vs GG: OR = 1.62, 95% CI = 1.02–2.59; A vs G: OR = 1.48, 95% CI = 1.02–2.16). In the meanwhile, CCR5 rs1800024 polymorphism also exhibited significant differences in genotype and allele distribution (P < .05), demonstrating its promoting effect on the risk of PV under heterozygous model (OR = 1.73, 95% CI = 1.06–2.82), dominance model (OR = 1.83, 95% CI = 1.14–2.94), and allele model (OR = 1.68, 95% CI = 1.13–2.48).CCR2 rs1799864 and CCR5 rs1800024 polymorphisms may function as independent risk factors for PV in Chinese population.