Identification of SNP(Single Nucleotide Polymorphism) from MC1R, MITF and TYRP1 associated with Feather Color in Chicken

Abstract

ABSTRAT The Feather Color of chicken is considered as most obvious, and the purpose of this study is to identify the genotype following the SNP of MC1R, MITF and TYRP1, which are genes related to Feather Color, and develop a SNP marker that can be classified per breed. When a haplotype is observed through the combination of markers, a Korean Native Chicken can especially be distinguished when it is a CGG type in the SNP combination of the MC1R gene. In case of the TAG, TGG and TAA types, only Araucana was identified, and for the CAA type, Leghorn could specifically be distinguished. In the SNP combination of TYRP1 gene, only Leghorn was differentiated in case of the TTTCA and CCTCA types, and only Silky Fowl was identified in case of the CTTTA type. The SNP combination of MC1R gene enabled for Korean Native Chicken, Leghorn, and Araucana to be distinguished and each of the SNP and combination of TYRP1 gene allowed for all 4 breeds to be classified. If many researches are conducted about genetic polymorphism between breeds, then it is considered that the differences between breeds will be understood from a molecular biological aspect instead of simply distinguishing the breeds through Feather Color.

DNA Markers for the Genetic Diversity in Korean Native Chicken Breeds: A Review

“…D-loop control region은 non-coding region으로 기능 적인 역할을 담당하지 않고 변이가 다른 영역보다 두드러지 기 때문에 많이 활용되었고, COI 유전자는 주로 조류의 품종 구분 연구에 주로 활용되어 왔다 (Hebert et al, 2003 (Cheng and Crittenden, 1994;Kaya and Yildz, 2008 (Kim et al, 2004;Cahyadi et al, 2013). 특히, MC1R 유전자는 다양한 축종에서 피모색 과 관련된 유전변이를 찾고자 연구가 수행되어져 왔다 (Kerje et al, 2003;Maklund et al, 1996;Vage et al, 1999) Heo 등 (2011) Hoque et al (2013), Kim et al (2014) c.212C>T Nonsynonymous Heo et al (2011, Jin et al (2014) c.274A>G…”

“…Nonsynonymous Heo et al (2011), Hoque et al (2013, Park et al (2013), Jin et al (2014), Kim et al (2014) c.636G>A Synonymous Park et al (2013) c.637T>C Synonymous Park et al (2013) Variation in (Seo et al, 2015a;Wallis et al, 2004). 또한 방대한 re-sequencing data 를 이용해서 60K, 600K 등의 high-density(HD) SNP chip이 개발되어 많은 유전자 마커의 정보를 획득할 수 있게 되었 다 (Groenen et al, 2011;Kranis et al, 2013 …”