Identification of six sibling species of the Anopheles maculipennis complex (Diptera: Culicidae) by a polymerase chain reaction assay

Proft, Jana; Maier, Walter; Kampen, Helge

doi:10.1007/s004360050642

Cited by 152 publications

(135 citation statements)

References 23 publications

Supporting

Mentioning

124

Contrasting

Unclassified

Order By: Relevance

“…Les anophèles appartenant au complexe An. maculipennis ont été identifiés morphologiquement comme décrit par Brunhes et al (2000) puis par PCR adapté par Proft et al (1999).…”

Section: Matériel Et Méthodesunclassified

Risque de réémergence du paludisme au Maroc Étude de la capacité vectorielle d’Anopheles labranchiaedans une zone rizicole au nord du pays

et al. 2008

View full text Add to dashboard Cite

To assess the malaria reintroduction risk in Morocco, we analyzed the malariogenic potential of a rice cultivation area in the north of the country. Our results showed that the receptivity of this area is very high during all the period of the rice cultivation, from May to October. the vectorial capacity of An. labranchiae, malaria vector in Morocco, is considerably high during the summer which corresponds to the rice cultivation period. The risk of autochthonous malaria resumption is important because of the possible presence of gametocytes carriers in the last malaria focus which is bordering the study area. The risk of a tropical malaria introduction is unimportant seen the low vulnerability of the area and the uncertain competence of its vectors considered. However, this risk must be considered with a more attention. Résumé

show abstract

“…Les anophèles appartenant au complexe An. maculipennis ont été identifiés morphologiquement comme décrit par Brunhes et al (2000) puis par PCR adapté par Proft et al (1999).…”

Section: Matériel Et Méthodesunclassified

Risque de réémergence du paludisme au Maroc Étude de la capacité vectorielle d’Anopheles labranchiaedans une zone rizicole au nord du pays

et al. 2008

View full text Add to dashboard Cite

show abstract

“…(Cellia) gambiae Giles complex (Scott et al 1993) and the Holarctic An. (Anopheles) maculipennis Meigen group (Cornel et al 1996, Proft et al 1999. ITS2 sequences have also been used in taxonomic studies of closely related species in the subgenus Nyssoryhnchus, including An.…”

mentioning

confidence: 99%

Molecular differentiation of Anopheles (Nyssorhynchus) benarrochi and An. (N.) oswaldoi from Southern Colombia

Ruiz

Quiñónes

Erazo

et al. 2005

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

Anopheles (Nyssorhynchus) benarrochi, An. (N.) oswaldoi, and An. (N.) rangeli are the most common anthropophilic mosquitoes in the southern Colombian state of Putumayo. Adult females are most commonly collected in epidemiological studies, and this stage poses significant problems for correct identification, due to overlapping inter-specific morphological characters. Although An. rangeli is easy to identify, the morphological variant of An. benarrochi found in the region and An. oswaldoi are not always easy to separate. Herein we provide a rapid molecular method to distinguish these two species in Southern Colombia. Sequence data for the second internal transcribed spacer (ITS2) region of rDNA was generated for link-reared progeny of An. benarrochi and An. oswaldoi, that had been identified using all life stages. ITS2 sequences were 540 bp in length in An. benarrochi (n = 9) and 531 bp in An. oswaldoi (n = 7). Sequences showed no intra-specific variation and ungapped inter-specific sequence divergence was 6.4%. Species diagnostic banding patterns were recovered following digestion of the ITS2 amplicons with the enzyme Hae III as follows: An. benarrochi (365, 137, and 38 bp) and An. oswaldoi (493 and 38 bp). This polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay provides rapid, accurate, and inexpensive species diagnosis of adult females. This will benefit future epidemiological studies and, as PCR amplification can be achieved using a single mosquito leg, the remaining specimen can be either retained as a morphological voucher or further used in vector incrimination studies. That An. benarrochi comprises a complex of at least two species across Latin America is discussed

show abstract

“…The ITS-2 rDNA is even the usual key marker for the differentiation of problematic taxa, as in cryptic and sibling species of mosquitoes [22][23][24][25]51,52]. The length and GC content of this spacer for Cx.…”

Section: Discussionmentioning

confidence: 99%

“…The molecular markers used for the characterisation of Cx. theileri were selected according to previous results having shown their usefulness for mosquitoes in general or Culicids in particular: small subunit or 18S rRNA gene [18][19][20][21] and the second internal transcribed spacer ITS-2 [22][23][24][25] within the nuclear rDNA, and the cytochrome c oxidase subunit I cox1 [9, within the mtDNA. This finding represents a new record for vector species not only for the Canary Island fauna but also for the whole Spanish country where the only mosquito species involved in D.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Culex Theileri from Canary Islands, Spain, a Potential Vector of Dirofilaria Immitis

Bargues¹

2013

J Clin Exp Pathol

View full text Add to dashboard Cite

Dirofilaria immitis is the causal agent of heartworm diseases and of human pulmonary dirofilariosis. The infection is transmitted by several species of culicid mosquitoes that are frequently able to bite both animal reservoirs and humans. Canary Islands (Spain) constitute a well documented endemic area of canine dirofilariosis in which the mosquito species involved in the transmission of D. immitis are not known. The objectives of the present work were the identification of vectors of this parasite in Canary Islands and their molecular characterization. A total of 1219 female mosquitoes were captured. The most abundant species was Culex theileri (52.26%) followed by Cx. pipiens (35.44%), Anopheles cinereus hispaniola (6.23%), Culiseta longiareolata (5.74%), and Culex laticintus (0.33%). PCR was applied for the detection of larval D. immitis DNA in mosquitoes. D. immitis DNA was observed in the abdomen of one Cx. theileri female: 0.082% of the entire mosquito population and 0.17% in Cx. theileri. A molecular identification of Cx. theileri, the potential mosquito vector of dirofilariosis in this zoonotic focus in Canary Islands of Spain, has been made for first time based on sequences of the 18S rRNA gene, the second internal transcribed spacer (ITS2) of ribosomal DNA and the barcode region of the cytochrome c oxidase I (cox1) gene of mitochondrial DNA, allowing a broad mosquito molecular basis for future populations genetic analyses of this vector species. Parasitological and entomological molecular results suggest that Cx. theileri is a potential natural vector of D. immitis in Canary Islands.

show abstract

Identification of six sibling species of the Anopheles maculipennis complex (Diptera: Culicidae) by a polymerase chain reaction assay

Cited by 152 publications

References 23 publications

Risque de réémergence du paludisme au Maroc Étude de la capacité vectorielle d’Anopheles labranchiaedans une zone rizicole au nord du pays

Risque de réémergence du paludisme au Maroc Étude de la capacité vectorielle d’Anopheles labranchiaedans une zone rizicole au nord du pays

Molecular differentiation of Anopheles (Nyssorhynchus) benarrochi and An. (N.) oswaldoi from Southern Colombia

Molecular Characterization of Culex Theileri from Canary Islands, Spain, a Potential Vector of Dirofilaria Immitis

Contact Info

Product

Resources

About