Identification of Single Nucleotide Polymorphisms in BMP 15 Gene in Egyptian buffaloes

Araby, Iman E. El; Saleh, Ayman A.; othman, Hoda; Awad, Ashraf; El‐fattah, Amir H. Abd; El-Magd, Mohammed Abu

doi:10.5455/ajvs.224255

Cited by 2 publications

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“…Among oocyte-derived BMP family members, two proteins including GDF9 and BMP15 have played essential roles in female fertility for several mammalian species [4,5] and are key regulators of follicle development, ovulation rate, and oocyte quality [6]. These proteins are members of the transforming growth factor β (TGFβ) superfamily members that are pivotal in controlling cellular growth and differentiation during fetal and adult life [7], in the development of ovarian follicles, female reproductive tract differentiation, and organogenesis [8,9]. BMP15 is essential for folliculogenesis, female fertility, and inhibited LH stimulated androstenedione production in theca cells [10], which led to the severity of PCOS [11].…”

Section: Introductionmentioning

confidence: 99%

Association between polymorphism in BMP15 and GDF9 genes and impairing female fecundity in diabetes type 2

Al‐Shuhaib

2020

Middle East Fertil Soc J

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Background: A shortened reproductive period and earlier menopause have been associated with type 2 diabetes. Growth differentiation factor 9(GDF9) and bone morphogenetic protein 15 (BMP15) gene mutations have been associated with earlier menopause. Therefore, this study aimed to evaluate the association between BMP15 and GDF9 mutations with impairing female fecundity in diabetic patients. The study subjects comprised 90 female diabetic patients and 60 female healthy controls. The physio-biochemical analysis was measured using enzymatic determination. A single-strand conformation polymorphism (SSCP) protocol was utilized to assess the pattern of genetic variations. Results: Genotyping analysis of the BMP15 gene showed a heterogeneous pattern with the presence of two genotypes: AA and AC genotypes. Five novel missense single nucleotide polymorphisms (SNPs) were identified in the BMP15 gene: four SNPs detected in both genotypes, and Met4Leu, a specific SNP, was detected only in the AC genotype. Cumulative in silico tools indicated a highly deleterious effect for the Met4Leu on the mutant protein structure, function, and stability. Diabetes patients showed a significantly higher frequency of genotype AC. The physio-biochemical analysis of fasting plasma glucose (FBG), glycosylated hemoglobin (HbA1c), and luteinizing hormone (LH) were significantly higher (P < 0.05) in AC genotype than AA genotype. Conclusions: The current research provides the first indication regarding the tight association of BMP15 polymorphism with the impairing female fecundity in the diabetic. A pivotal role is played by the novel (Met4Leu) SNP that can be used as a predictor for the impairing female fecundity of diabetes, while no polymorphism was found in exon 4 of the GDF9 gene.

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Section: Introductionmentioning

confidence: 99%

Association between polymorphism in BMP15 and GDF9 genes and impairing female fecundity in diabetes type 2

Al‐Shuhaib

2020

Middle East Fertil Soc J

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Real-time PCR using atpE, conventional PCR targeting different regions of difference, and flow cytometry for confirmation of Mycobacterium bovis in buffaloes and cattle from the Delta area of Egypt

et al. 2022

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Background Mycobacterium bovis notoriously causes detrimental infections in bovines and humans. In this study, 1500 buffaloes and 2200 cattle were tested by single intradermal comparative cervical tuberculin test and compared with the detection rates of M. bovis isolation, real-time and simplex PCR, and flow Cytometry. Results The tuberculin test is the reference test in Egypt, the positive rate was 54/3700 (1.5%) composed of 18/1500 (1.2%) buffaloes and 36/2200 (1.6%) cattle which were mandatorily slaughtered under the Egyptian legislation, after postmortem examination the non-visible-lesion proportion was 39/54 (72.2%) which surpassed the visible-lesion rate 15/54 (27.8%) with (p < 0.0001). The samples from each case were pooled into one sample representing the case, and the isolation rate of M. bovis was 25/54 (46.3%). Real-time PCR using atpE was positive for mycobacteria on the genus level in 18/18 (100%) and 5/5 (100%) of tissue samples and isolates, respectively; simplex PCR detected M. bovis in 44/54 (81.5%) and 25/25 (100%) of tissue samples and isolates, respectively. Flow Cytometry evaluation of the CD4+, CD8+, WC1+δγ, and CD2+ cell phenotypes showed increased counts in the tuberculin-positive cases compared with negative cases (p < 0.0001), and these phenotypes in the tuberculin-positive cases increased after antigen stimulation than in the negative cases (p < 0.0001). Detection rates of PCR techniques and flow Cytometry exceeded that of bacterial isolation (p < 0.0001) and exhibited a strong correlation. Conclusions The skin test suffers from interference from non-tuberculous mycobacteria able to cause false-positive reactions in cattle and other species. Real-time PCR using atpE, conventional PCR targeting RDs, and flow Cytometry are rapid and accurate methods that correlate with the isolation and can be promising for detection and confirmation of infected live and slaughtered cases.

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Identification of Single Nucleotide Polymorphisms in BMP 15 Gene in Egyptian buffaloes

Cited by 2 publications

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Association between polymorphism in BMP15 and GDF9 genes and impairing female fecundity in diabetes type 2

Association between polymorphism in BMP15 and GDF9 genes and impairing female fecundity in diabetes type 2

Real-time PCR using atpE, conventional PCR targeting different regions of difference, and flow cytometry for confirmation of Mycobacterium bovis in buffaloes and cattle from the Delta area of Egypt

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