Identification of single major QTL and candidate gene(s) governing hull-less seed trait in pumpkin

Kaur, Barinder; Garcha, Karmvir Singh; Bhatia, Dharminder; Khosa, Jiffinvir Singh; Sharma, Madhu; Mittal, Amandeep; Verma, Neha; Dhatt, Ajmer Singh

doi:10.3389/fpls.2022.948106

Cited by 8 publications

(5 citation statements)

References 50 publications

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“…Therefore, we selected CMTp182 for the confirmation of hybridity of putative interspecific F 1 plants. In support to this, our recently published manuscript 22 identified a candidate genomic region linked to hull-less seed trait ranging from 1.80 to 3.86 Mb on chromosome 12 of C. pepo genome and also reported a linked KASP marker. Similar results are also reported by two other recently published studies in C. pepo 23 , 24 .…”

Section: Discussionmentioning

confidence: 60%

“…These results are similar to the previous reports in C. pepo by Stuart 40 , Zraidi et al 41 and Lelley et al 3 . More recently, along with monogenic recessive inheritance, hull-less seed trait has been mapped to chromosome 12 of C. pepo genome with 'NST1' (Cp4.1Lg12g04350) as the putative candidate gene [22][23][24] . However, segregation distortion observed in Family IV, might be due to incomplete representation of whole F 2 population in chi square analysis.…”

Section: Total Number Of F 2 Seeds Sownmentioning

confidence: 99%

“…It has also been reported in several interspecific crosses of rice 43 , wheat 44 , cotton 45 and potato 46 . The distortions observed in the F 2 generation among marker-phenotype associations might be due to the physical distance of marker from hull-less responsible gene [22][23][24] . Moreover, these distortions are of regular occurrence in early generations of wide crosses due to genomic instabilities or meiotic distortions 47,48 .…”

Section: Total Number Of F 2 Seeds Sownmentioning

confidence: 99%

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Interspecific hybridization for transfer of hull-less seed trait from Cucurbita pepo to C. moschata

Kaur

Garcha

Sandhu

et al. 2023

Sci Rep

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Hull-less seed trait is preferred by nut and oil industries worldwide for snacking and oil extraction as it evades the expensive decorticating (dehulling) process. This seed trait is available in C. pepo only, which has small seed cavity, sensitive to various biotic and abiotic stresses, and restricted to temperate regions for cultivation. Contrarily, the related species C. moschata has wider adaptability, disease tolerance and high seed yield. Therefore, attempt was made to transfer this trait into C. moschata through conventional pollination and ovule culture using four parents of hull-less C. pepo and six of hulled C. moschata. Through conventional approach, few viable F1 seeds (12–23) were obtained by using C. pepo as female parent, but in three crosses (HLP36 × HM1343, HLP36 × HM1022 and HLP44 × HM1022) only, whereas, its use as male parent was not successful. This incompatibility issue of reciprocals was resolved through ovule culture of C. moschata genotypes HM1343 and HM6711 after 17 to 19 days of pollination with C. pepo genotypes HLP53 and HLP72, respectively. The hybridity of interspecific crosses was confirmed through SSR markers (alleles inherited from both the parents), morphological characters and micromorphological leaf traits (differed from both the parents). The successful transfer through interspecific hybridization was further established with the presence of hull-less seed in fruits of F2 populations. Outcome of this study would pave the way for enhancing the productivity and multi-season cultivation of snack-seeded pumpkin even in subtropical and tropical regions.

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Section: Discussionmentioning

confidence: 60%

Section: Total Number Of F 2 Seeds Sownmentioning

confidence: 99%

Section: Total Number Of F 2 Seeds Sownmentioning

confidence: 99%

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Interspecific hybridization for transfer of hull-less seed trait from Cucurbita pepo to C. moschata

Kaur

Garcha

Sandhu

et al. 2023

Sci Rep

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“…Hull-less or naked seed mutants have been identified in all three Cucurbita species, which were all controlled by a single recessive gene (n or h) [2,[12][13][14]. The n locus in C. pepo has been fine-mapped, and the results from three independent studies all support the NAC transcription gene Cp4.1LG12g04350 (CpNST1) on Chromosome 12 of C. pepo as the most possible candidate gene [19,20,43]. CmoCh17G004790 (CmNST1) and Cp4.1LG12g04350 (CpNST1) are homologs in the C. pepo and C. moschata genomes (Supplemental File S2).…”

Section: Cmnst1 Is a Candidate Gene For The Naked Seed (N) Locusmentioning

confidence: 99%

Alternative Splicing of NAC Transcription Factor Gene CmNST1 Is Associated with Naked Seed Mutation in Pumpkin, Cucurbita moschata

Shen

Weng

2023

Genes

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In pumpkin (Cucurbita moschata), the naked or hull-less seed phenotype has great benefits for breeding this crop for oil or snack use. We previously identified a naked seed mutant in this crop. In this study, we report genetic mapping, identification, and characterization of a candidate gene for this mutation. We showed that the naked seed phenotype is controlled by a single recessive gene (N). The bulked segregant analysis identified a 2.4 Mb region on Chromosome 17 with 15 predicted genes. Multiple lines of evidence suggested that CmoCh17G004790 is the most probable candidate gene for the N locus which encodes a NAC transcription factor WALL THICKENING PROMOTING FACTOR 1 (CmNST1). No nucleotide polymorphism or structural variation was found in the genomic DNA sequences of CmNST1 between the mutant and the wildtype inbred line (hulled seed). However, the cDNA sequence cloned from developing seed coat samples of the naked seed mutant was 112 bp shorter than that from the wildtype which is due to seed coat-specific alternative splicing in the second exon of the mutant CmNST1 transcript. The expression level of CmNST1 in the developing seed coat was higher in the mutant than in the wildtype during early seed coat development which was reversed later. Transcriptomic profiling with RNA-Seq at different stages of seed development in the mutant and wildtype revealed a critical role of CmNST1 as a master regulator for the lignin biosynthesis pathway during seed coat development while other NAC and MYB transcription factors were also involved in forming a regulatory network for the building of secondary cell walls. This work provides a novel mechanism for the well-characterized NST1 transcription factor gene in regulating secondary cell wall development. The cloned gene also provides a useful tool for marker-assisted breeding of hull-less C. moschata varieties.

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“…durum-Aegilops longissima with high iron and zinc contents (Tiwari et al, 2008) and T. turgidum-Aegilops umbellulata with strong tillering ability, stripe rust resistance, and seed size-related traits (Song et al, 2019) have also been reported. Similarly in vegetables, the hull-less seed trait was transferred from Cucurbita pepo to Cucurbita moschata through interspecific hybridization (Kaur et al, 2023).…”

Section: Selfed Amphidiploid Derivativesmentioning

confidence: 99%

Genetic enhancement of okra [Abelmoschus esculentus (L.) Moench] germplasm through wide hybridization

Suma,

Joseph John,

Bhat

et al. 2023

Front. Plant Sci.

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IntroductionThe introgression of genetic material from one species to another through wide hybridization and repeated back-crossing, plays an important role in genetic modification and enriching the cultivated gene-pool with novel genetic variations. Okra (Abelmoschus esculentus [(L.) Moench)] is a popular vegetable crop with high dietary fibre and protein, rich in essential amino acids, lysine and tryptophan. The wild Abelmoschus genepool has many desirable traits like ornamental value, short internodal length, more number of productive branches, extended bearing, perennation tendency, reduced fruit length (more consumer preferred trait), high mucilage content (medicinal value), abiotic stress tolerances such as drought, high temperature and biotic stress resistances such as okra Yellow Vein Mosaic Virus (YVMV) and Enation Leaf Curl Virus (ELCV) diseases. The repeated use of elite breeding lines led to narrowing of the genetic base of the okra crop, one of the major factors attributed to breakdown of resistance/ tolerance to biotic stresses. YVMV and ELCV are the two major diseases, causing significant yield loss in okra. Hence, wide hybridization was attempted to transfer tolerance genes from wild species to the cultivated genepool to widen the genetic base.Material and methodsThe screening of germplasm of wild Abelmoschus species at hotspots led to the identification of tolerant species (Abelmoschus pungens var. mizoramensis, A. enbeepeegeearensis, A. caillei, A. tetraphyllus and A. angulosus var. grandiflorus), which were further used in a wide-hybridization programme to generate interspecific hybrids with the cultivated okra. Presence of pre- and post-zygotic barriers to interspecific geneflow, differences in ploidy levels and genotype specific variations in chromosome numbers led to varying degrees of sterility in F1 plants of interspecific crosses. This was overcome by doubling the chromosome number of interspecific hybrids by applying Colchicine at the seedling stage. The 113 cross derivatives generated comprising amphidiploids in the F1 generation (30), F3 (14), one each in F2 and F4 generations, back cross generation in BC1F2 (03), BC1F3 (25), and BC2F3 (02), crosses between amphidiploids (27), multi-cross combinations (07) and inter-specific cross (between A. sagittifolius × A. moschatus subsp. moschatus) selfed derivatives at F8 generation (03) were characterized in the present study. Besides they were advanced through selfing and backcrossing.Results and DiscussionThe amphidiploids were found to possess many desirable genes with a considerable magnitude of linkage drag. Majority of the wide cross derivatives had an intermediate fruit morphology and dominance of wild characters viz., hispid fruits, stem, leaves, tough fruit fibre, vigorous perennial growth habit and prolonged flowering and fruiting. The fruit morphology of three BC progenies exhibited a high morphological resemblance to the cultivated okra, confirming successful transfer of useful genes to the cultivated okra genepool. The detailed morphological characteristics of the various combinations of Abelmoschus amphidiploids and the genetic enhancement of the genepool achieved in this process is reported here.

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Identification of single major QTL and candidate gene(s) governing hull-less seed trait in pumpkin

Cited by 8 publications

References 50 publications

Interspecific hybridization for transfer of hull-less seed trait from Cucurbita pepo to C. moschata

Interspecific hybridization for transfer of hull-less seed trait from Cucurbita pepo to C. moschata

Alternative Splicing of NAC Transcription Factor Gene CmNST1 Is Associated with Naked Seed Mutation in Pumpkin, Cucurbita moschata

Genetic enhancement of okra [Abelmoschus esculentus (L.) Moench] germplasm through wide hybridization

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