Identification of Silver Nanoparticles in the Small Intestinal Mucosa, Liver, and Spleen of Rats by Transmission Electron Microscopy

Abstract

The effects of water-dispersed Ag nanoparticles on the small intestinal mucosa, liver, and spleen of rats were studied by transmission electron microscopy. Acute experiments demonstrated penetration of Ag nanoparticles injected into the isolated intestinal loop into the intestinal mucosa, liver, and splenic tissues. Ultrastructural changes (lobed nucleus, megamitochondria) were found in the studied organs. These data indicated that injection of water-dispersed Ag nanoparticles into the gastrointestinal tract was followed by their penetration through the epithelium of the small intestinal mucosa into other organs, e.g. into the liver and spleen. This fact is essential for evaluation of potential risks of the nanoparticle effects on human health and environment.

Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio

“…Interestingly, even with the significant accumulation in the tissue, minimal damage was observed. The lack of damage could be attributed to the considerable amount of coating agent, enhanced by its high clearance rate, as we have shown in previous works [18,21,[64][65][66][67]. These features definitely contribute to the wide therapeutic window found for the five formulations assessed in this work.…”

Analysis of hematologic alterations, immune responses and metallothionein gene expression in Nile tilapia (Oreochromis niloticus) exposed to silver nanoparticles

“…Platonova et al (2013) noted accumulation of AgNP in the liver and spleen of rats after injection of AgNP solution. In adult zebrafish exposed to AgNP, accumulation was also noted in the liver (Choi et al 2010).…”

Hemolysis of Human Erythrocytes by Argovit™ AgNPs from Healthy and Diabetic Donors: An In Vitro Study

“…The effective concentrations employed attributed to the high stability provided by the high amount of PVP used to obtain this AgNP formulation. Besides, biodistribution studies on rats show that 98% of this formulation was excreted in the feces [ 24 , 25 , 26 ], a result that confirms its low toxicity. To better understand the toxicological profile, an evaluation of the hemolytic effect of this formulation on human erythrocytes is indispensable.…”