Identification of side population cells (stem-like cell population) in pediatric solid tumor cell lines

Komuro, Hiroaki; Saihara, Ryoko; Shinya, Miki; Takita, Junko; Kaneko, Sawa; Kaneko, Michio; Hayashi, Yasuhide

doi:10.1016/j.jpedsurg.2007.08.026

Cited by 53 publications

(47 citation statements)

References 31 publications

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“…Further studies suggest that CSCs markers are composed of CD133 (Immervoll et al, 2008;Jaggupilli and Elkord, 2012), CD24 (Jaggupilli and Elkord, 2012), CD90, CD34, CD117 and CD20 as other potential CSC markers in bladder carcinomas (Komuro et al, 2007;Mehrazma et al, 2013). CD44v6+/EMA-cells were used for isolating bladder CSCs in some studies (Yang and Chang, 2008;Chan et al, 2010;Ho et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

ALDH1 in Combination with CD44 as Putative Cancer Stem Cell Markers are Correlated with Poor Prognosis in Urothelial Carcinoma of the Urinary Bladder

Keymoosi¹,

Gheytanchi²,

Asgari³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Section: Introductionmentioning

confidence: 99%

ALDH1 in Combination with CD44 as Putative Cancer Stem Cell Markers are Correlated with Poor Prognosis in Urothelial Carcinoma of the Urinary Bladder

Keymoosi¹,

Gheytanchi²,

Asgari³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…ABCG2 contributes to the exclusion of Hoechst in SP cells (Komuro et al, 2007) and can be a stem cell marker (Ding et al, 2010). Therefore, decrease in ABCG2 expression is consistent with the decrease in the percentage of SP cells.…”

Section: Discussionmentioning

confidence: 58%

“…Samples were washed, centrifuged, and resuspended in cold RPMI 1640 medium supplemented with 2% FBS, and then, 1 mg/mL propidium iodide (Sigma) was added. The FCM analysis and sorting of SP cells were performed using a BD FACSAria II flow cytometer (BD Biosciences, USA) as previously described (Komuro et al, 2007).…”

Section: Fcm Analysis and Sorting Of Sp Cellsmentioning

confidence: 99%

Effects of bisphenol A on decreasing the percentage and promoting the growth of stem cell-like cells from SK-N-SH human neuroblastoma cells

Zheng¹,

Qi²,

Yang³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Bisphenol A (BPA) is an industrial contaminant and is reported to be a risk factor associated with the development of tumors. In our previous studies, we have shown that BPA promoted the growth of SK-N-SH human neuroblastoma cells and increased their invasion and metastasis. In this study, we further investigated the effects of BPA and 17b-estradiol (E 2 ) on the stem cell-like cells from SK-N-SH cells. Detection of stem cell markers, proliferation assay, and clonogenic analysis showed that the side-population (SP) of SK-N-SH cells had properties similar to those of stem cells. BPA or E 2 exposure decreased the percentage of SP cells and the expression of stem cell-marker proteins. BPA and E 2 promoted the growth of non-SP cells to a greater extent than of SP cells; in addition, they significantly increased the 2987 BPA promotes the growth of cancer stem cell-like SP cells ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 2986-2993 (2015 growth of SP cells. Thus, BPA has effects on stem cell-like cells, which induce tumor formation, and thus, BPA is an environmental factor that plays an important role in the development of neuroblastoma.

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“…[2] Functional CSC properties like intracellular ALDH enzymatic activities and ABC transporter efflux activities of vital DNA dyes such as Hoechst 33342 have been used for CSC isolation. [21,22] Increased aldehyde dehydrogenase isoform 1 (ALDH1) activity has been used to identify and analyze different types of CSCs. Furthermore, CSCs have a distinct efflux mechanism, stemming from their high expression of ABC transporter proteins.…”

Section: Cd38mentioning

confidence: 99%

Therapeutic strategies for targeting cancer stem cells

Kim¹,

Siegler²,

Siriwon³

et al. 2016

JCMT

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The therapeutic limitations of conventional chemotherapeutic drugs present a challenge for cancer therapy; these shortcomings are largely attributed to the ability of cancer cells to repopulate and metastasize after initial therapies. Compelling evidence suggests that cancer stem cells (CSCs) have a crucial impact in current shortcomings of cancer therapy because they are largely responsible for tumor initiation, relapse, metastasis, and chemo-resistance. Thus, a better understanding of the properties and mechanisms underlying CSC resistance to treatments is necessary to improve patient outcomes and survival rates. In this review, the authors characterize and compare different CSC-specific biomarkers that are present in various types of tumors. We further discuss multiple targeting approaches currently in preclinical or clinical testing that show great potential for targeting CSCs. This review discusses numerous strategies to eliminate CSCs by targeting surface biomarkers, regulating CSC-associated oncogenes and signaling pathways, inhibiting drug-efflux pumps involved in drug resistance, modulating the tumor microenvironment and immune system, and applying drug combination therapy using nanomedicine.

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Identification of side population cells (stem-like cell population) in pediatric solid tumor cell lines

Abstract: This study suggested that most PSTs would contain a small fraction of SP cells (possible stem-like population). Targeting the CSC will provide a novel treatment strategy to eradicate refractory PSTs.

Cited by 53 publications

References 31 publications

ALDH1 in Combination with CD44 as Putative Cancer Stem Cell Markers are Correlated with Poor Prognosis in Urothelial Carcinoma of the Urinary Bladder

ALDH1 in Combination with CD44 as Putative Cancer Stem Cell Markers are Correlated with Poor Prognosis in Urothelial Carcinoma of the Urinary Bladder

Effects of bisphenol A on decreasing the percentage and promoting the growth of stem cell-like cells from SK-N-SH human neuroblastoma cells

Therapeutic strategies for targeting cancer stem cells

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