Effects of bisphenol A on decreasing the percentage and promoting the growth of stem cell-like cells from SK-N-SH human neuroblastoma cells

Zheng, Jicui; Qi, Shimei; Yang, Lin; Y, Y; Dong, Ke; Zhu, Haitao; Yang, Shaobo; Xu, Tao; Zheng, Shan; Xiao, Xianmin

doi:10.4238/2015.april.10.8

Cited by 6 publications

(3 citation statements)

References 27 publications

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“…This statistically significant result concerns all types of tumour combined, which limits the value of these results. However, it is important to highlight that reviewing MACAPEMUR database we found 4 out of the 8 cases observed are neuroectodermal or derived from the neural crest, and there are previous studies that have found associations between SNST and exposures to environmental toxicants such as bisphenol A, polycyclic aromatic hydrocarbons or carbon tetrachloride (Heck et al, 2013; Zheng et al, 2015). Muirhead et al (2015) describe in their study that neuroblastic tumours occur in mini-epidemics where transient environmental exposures, such as infections or air pollution, are involved.…”

Section: Discussionmentioning

confidence: 81%

Childhood cancer in small geographical areas and proximity to air-polluting industries

Ortega-García

Hernández

Cárceles-Álvarez

et al. 2017

Environmental Research

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Aim Pediatric cancer has been associated with exposure to certain environmental carcinogens. The purpose of this work is to analyse the relationship between environmental pollution and pediatric cancer risk. Method We analysed all incidences of pediatric cancer (< 15) diagnosed in a Spanish region during the period 1998–2015. The place of residence of each patient and the exact geographical coordinates of main industrial facilities was codified in order to analyse the spatial distribution of cases of cancer in relation to industrial areas. Focal tests and focused Scan methodology were used for the identification of high-incidence-rate spatial clusters around the main industrial pollution foci. Results The crude rate for the period was 148.0 cases per 1,000,0000 children. The incidence of pediatric cancer increased significantly along the period of study. With respect to spatial distribution, results showed significant high incidence around some industrial pollution foci group and the Scan methodology identify spatial clustering. We observe a global major incidence of non Hodgkin lymphomas (NHL) considering all foci, and high incidence of Sympathetic Nervous System Tumour (SNST) around Energy and Electric and organic and inorganic chemical industries foci group. In the analysis foci to foci, the focused Scan test identifies several significant spatial clusters. Particularly, three significant clusters were identified: the first of SNST was around energy-generating chemical industries (2 cases versus the expected 0.26), another of NHL was around residue-valorisation plants (5 cases versus the expected 0.91) and finally one cluster of Hodgkin lymphoma around building materials (3 cases versus the expected 2.2) Conclusion Results suggest a possible association between proximity to certain industries and pediatric cancer risk. More evidences are necessary before establishing the relationship between industrial pollution and pediatric cancer incidence.

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Section: Discussionmentioning

confidence: 81%

Childhood cancer in small geographical areas and proximity to air-polluting industries

Ortega-García

Hernández

Cárceles-Álvarez

et al. 2017

Environmental Research

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“…In the neuroblastoma cell line SK‐N‐SH, for example, adding 3.75 to 5 µg mL −1 Hoechst significantly induces cell death. [ 20 ] A total of 5 µg mL −1 Hoechst reduces the cell viability of HL‐60 cells to 30% after 24 h. [ 21 ] Erba et al. reported cytotoxic effects of 1.5 µg mL −1 Hoechst on L1210 cells.…”

Section: Discussionmentioning

confidence: 99%

Breaking a Dogma: High‐Throughput Live‐Cell Imaging in Real‐Time with Hoechst 33342

Fuchs

Jahn

et al. 2023

Adv Healthcare Materials

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Automated high‐throughput live cell imaging (LCI) enables investigation of substance effects on cells in vitro. Usually, cell number is analyzed by phase‐contrast imaging, which is reliable only for a few cell types. Therefore, an accurate cell counting method, such as staining the nuclei with Hoechst 33342 before LCI, will be desirable. However, since the mid‐1980s, the dogma exists that Hoechst can only be used for endpoint analyses because of its cytotoxic properties and the potentially phototoxic effects of the excitation light. Since microscopic camera sensitivity has significantly improved, this study investigates whether this dogma is still justified. Therefore, exposure parameters are optimized using a 4× objective, and the minimum required Hoechst concentration is evaluated, allowing LCI at 30‐min intervals over 5 days. Remarkably, a Hoechst concentration of only 57 × 10−9 m significantly inhibits proliferation and thus impairs cell viability. However, Hoechst concentrations between 7 × 10−9 and 28 × 10−9 m can be determined, which are neither cytotoxic nor impacting cell viability, proliferation, or signaling pathways. The method can be adapted to regular inverted fluorescence microscopes and allows, for example, to determine the cytotoxicity of a substance or the transduction efficiency, with the advantage that the analysis can be repeated at any desired time point.

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“…Low‐dose BPA exposure results in structural and behavioral changes in the brain, such as decreased dendritic spine density, impaired memory, altered hypothalamic pituitary adrenal (HPA) axis, altered levels of neuronal nitric oxide synthase (nNOS) and steroid receptor, induced aggressiveness, and anxiety . In vivo and in vitro growth‐promoting effects of BPA on SK‐N‐SH cells have also been reported …”

Section: Introductionmentioning

confidence: 99%

Low‐dose bisphenol A induces RIPK1‐mediated necroptosis in SH‐SY5Y cells: Effects on TNF‐α and acetylcholinesterase

Ayazgök

Küçükkılınç

2018

J Biochem & Molecular Tox

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Bisphenol A (BPA) is an endocrine disruptor chemical, which is commonly used in everyday products. Adverse effects of its exposure are reported even at picomolar doses. Effects of picomolar and nanomolar concentrations of BPA on cytotoxicity, nitric oxide (NO) levels, acetylcholinesterase (AChE) gene expression and activity, and tumor necrosis factor‐α (TNF‐α) and caspase‐8 levels were determined in SH‐SY5Y cells. The current study reveals that low‐dose BPA treatment induced cytotoxicity, NO, and caspase‐8 levels in SH‐SY5Y cells. We also evaluated the mechanism underlying BPA‐induced cell death. Ours is the first report that receptor‐interacting serine/threonine‐protein kinase 1–mediated necroptosis is induced by nanomolar BPA treatment in SH‐SY5Y cells. This effect is mediated by altered AChE and decreased TNF‐α levels, which result in an apoptosis‐necroptosis switch. Moreover, our study reveals that BPA is an activator of AChE.

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Effects of bisphenol A on decreasing the percentage and promoting the growth of stem cell-like cells from SK-N-SH human neuroblastoma cells

Cited by 6 publications

References 27 publications

Childhood cancer in small geographical areas and proximity to air-polluting industries

Childhood cancer in small geographical areas and proximity to air-polluting industries

Breaking a Dogma: High‐Throughput Live‐Cell Imaging in Real‐Time with Hoechst 33342

Low‐dose bisphenol A induces RIPK1‐mediated necroptosis in SH‐SY5Y cells: Effects on TNF‐α and acetylcholinesterase

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