Identification of sheep lncRNAs related to the immune response to vaccines and aluminium adjuvants

Bilbao-Arribas, Martin; Varela-Martínez, Endika; Abendaño, Naiara; Andrés, D. de; Luján, Lluís; Jugo, Begoña M.

doi:10.1186/s12864-021-08086-z

Cited by 2 publications

(2 citation statements)

References 77 publications

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“…Though, in encephalon, the response was very mild, and a greater gene alteration was observed in sheep treated only with aluminum hydroxide. We also analyzed the differentially expressed lncRNAs in both tissues ( 9 , 10 ), and it was observed that many immune-related lncRNAs were deregulated in PBMC after repetitive vaccination.…”

Section: Introductionmentioning

confidence: 99%

Identifying transcriptomic profiles in ovine spleen after repetitive vaccination

Guisasola-Serrano,

Bilbao-Arribas,

Varela-Martínez

et al. 2024

Front. Immunol.

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Aluminum hydroxide has long been employed as a vaccine adjuvant for its safety profile, although its precise mechanism of action remains elusive. In this study, we investigated the transcriptomic responses in sheep spleen following repetitive vaccination with aluminum adjuvanted vaccines and aluminum hydroxide alone. Notably, this work represents the first exploration of the sheep spleen transcriptome in such conditions. Animals were splitted in 3 treatment groups: vaccine group, adjuvant alone group and control group. A total of 18 high-depth RNA-seq libraries were sequenced, resulting in a rich dataset which also allowed isoform-level analysis. The comparisons between vaccine-treated and control groups (V vs C) as well as between vaccine-treated and adjuvant-alone groups (V vs A) revealed significant alterations in gene expression profiles, including protein coding genes and long non-coding RNAs. Among the differentially expressed genes, many were associated with processes such as endoplasmic reticulum (ER) stress, immune response and cell cycle. The analysis of co-expression modules further indicated a correlation between vaccine treatment and genes related to ER stress and unfolded protein response. Surprisingly, adjuvant-alone treatment had little impact on the spleen transcriptome. Additionally, the role of alternative splicing in the immune response was explored. We identified isoform switches in genes associated with immune regulation and inflammation, potentially influencing protein function. In conclusion, this study provides valuable insights into the transcriptomic changes in sheep spleen following vaccination with aluminum adjuvanted vaccines and aluminum hydroxide alone. These findings shed light on the molecular mechanisms underlying vaccine-induced immune responses and emphasize the significance of antigenic components in aluminum adjuvant mechanism of action. Furthermore, the analysis of alternative splicing revealed an additional layer of complexity in the immune response to vaccination in a livestock species.

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Section: Introductionmentioning

confidence: 99%

Identifying transcriptomic profiles in ovine spleen after repetitive vaccination

Guisasola-Serrano,

Bilbao-Arribas,

Varela-Martínez

et al. 2024

Front. Immunol.

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“…The annotation and functional characterisation of livestock lncRNAs is essential, since most trait-associated variants in livestock lie within non-coding genome regions ( Weikard et al, 2017 ). In sheep, lncRNAs have been profiled across a multi-tissue dataset ( Bush et al, 2018 ), but there are few functional studies investigating their involvement in the immune response and those are difficult to compare due to differences in naming and data availability ( Jin et al, 2018 ; Bilbao-Arribas et al, 2021 ; Chitneedi et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic meta-analysis reveals unannotated long non-coding RNAs related to the immune response in sheep

Bilbao-Arribas

Jugo

2022

Front. Genet.

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Long non-coding RNAs (lncRNAs) are involved in several biological processes, including the immune system response to pathogens and vaccines. The annotation and functional characterization of lncRNAs is more advanced in humans than in livestock species. Here, we take advantage of the increasing number of high-throughput functional experiments deposited in public databases in order to uniformly analyse, profile unannotated lncRNAs and integrate 422 ovine RNA-seq samples from the ovine immune system. We identified 12302 unannotated lncRNA genes with support from independent CAGE-seq and histone modification ChIP-seq assays. Unannotated lncRNAs showed low expression levels and sequence conservation across other mammal species. There were differences in expression levels depending on the genomic location-based lncRNA classification. Differential expression analyses between unstimulated and samples stimulated with pathogen infection or vaccination resulted in hundreds of lncRNAs with changed expression. Gene co-expression analyses revealed immune gene-enriched clusters associated with immune system activation and related to interferon signalling, antiviral response or endoplasmic reticulum stress. Besides, differential co-expression networks were constructed in order to find condition-specific relationships between coding genes and lncRNAs. Overall, using a diverse set of immune system samples and bioinformatic approaches we identify several ovine lncRNAs associated with the response to an external stimulus. These findings help in the improvement of the ovine lncRNA catalogue and provide sheep-specific evidence for the implication in the general immune response for several lncRNAs.

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Identification of sheep lncRNAs related to the immune response to vaccines and aluminium adjuvants

Cited by 2 publications

References 77 publications

Identifying transcriptomic profiles in ovine spleen after repetitive vaccination

Identifying transcriptomic profiles in ovine spleen after repetitive vaccination

Transcriptomic meta-analysis reveals unannotated long non-coding RNAs related to the immune response in sheep

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