Identification of sex pheromone components of the pea midge, Contarinia pisi (Diptera: Cecidomyiidae)

Hillbur, Ylva; Anderson, Peter; Arn, H.; Bengtsson, Marie; Löfqvist, Jan; Biddle, A. J.; Smitt, Olof; Högberg, Hans‐Erik; Plass, Ernst; Franke, Stephan; Francke, Wittko

doi:10.1007/s001140050618

Cited by 25 publications

(21 citation statements)

References 8 publications

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“…Solvent extracts from virgin females have been found to attract conspecific males in six cecidomyiid species (Miller and Borden, 1984;Lee and Lee, 1985;Sain and Kalode, 1985;Wall et al, 1985;Williams and Martin, 1986;Pivnick, 1993). Sex pheromone components have been identified in three of these species (Miller et al, 1991;Hillbur et al, 1999;Gries et al, 2000). In the present study, all females showed calling behavior when they were placed on the original host plant, while few females showed this behavior on the wrong host plant (Table 3).…”

Section: Discussionsupporting

confidence: 51%

Host-associated differences in emergence pattern, reproductive behavior and life history of Asteralobia sasakii (Monzen) (Diptera: Cecidomyiidae) between populations on Ilex crenata and I. integra (Aquifoliaceae)

Tabuchi

Amano

2003

Appl. Entomol. Zool.

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We assessed potential adult longevity and male choice for a mate in two populations of Asteralobia sasakii associated with Ilex crenata and I. integra to test the hypothesis that interbreeding between these populations is prevented by the difference in emergence timing in the field. A previous study showed that the last day of adult emergence on I. integra and the first day of adult emergence on I. crenata were separated by six to 11 days. When adults were maintained in the laboratory with restricted flying, mating and oviposition, adults from both populations survived for four to five days, suggesting that the period when adults were active did not overlap between the two populations in the field. The mate choice test showed that males clearly approached females of the same host origin. These results suggest that the two populations have little chance of interbreeding even when they occur sympatrically. Other life history parameters such as the number of eggs in the ovary, daily emergence pattern, sex ratio and the duration of copulation also varied between the two populations. Our overall results strongly suggest that the two populations on different host plants can be referred to as distinct species.

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Section: Discussionsupporting

confidence: 51%

Host-associated differences in emergence pattern, reproductive behavior and life history of Asteralobia sasakii (Monzen) (Diptera: Cecidomyiidae) between populations on Ilex crenata and I. integra (Aquifoliaceae)

Tabuchi

Amano

2003

Appl. Entomol. Zool.

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“…For example, in C. oregonensis (Gries et al, 2002) and the aphidophagous gall midge, Aphidoletes aphidimyza (Choi et al, 2004), pheromone in extracts was undetectable by a mass spectrometer. Gland extracts of C. pisi contained a few picograms per female (Hillbur et al, 1999). Similarly, air entrainment of pheromone of D. mali gave approximately 20 pg per female (Cross and Hall, Cross andHall (2007, 2009, unpublished) 2007).…”

Section: Identification Of Sex Pheromonesmentioning

confidence: 97%

“…However, in the case of the raspberry cane midge, Resseliella theobaldi, air entrainment from 200 females yielded 1.5 μg of pheromone (i.e., 7.5 ng per female; Hall et al, 2009). Analysis by coupled gas chromatographic-electroantennographic detection (GC-EAD) has proved effective at detecting pheromone components in collections from female Cecidomyiidae, with the male antenna capable of detecting components below a GC threshold of detection (Hillbur et al, 1999;Gries et al, 2002;Choi et al, 2004). Specially designed Plexiglas holders have been used for the antennal preparation (Hillbur et al, 2001(Hillbur et al, , 2005Andersson et al, 2009a), while Gries et al (2002) used a severed head and antenna suspended between two glass electrodes.…”

Section: Identification Of Sex Pheromonesmentioning

confidence: 99%

“…In the original work on the major component of the pheromone of M. destructor, the absolute configuration of the pheromone, (10E)-2-acetoxy-10-tridecene, was determined by hydrolyzing extract, conversion to the acetyl (2S)-(+)-lactyl ester, and comparison of GC retention time with those of the diastereoisomers on a standard, non-polar GC column (Foster et al, 1991a). Subsequently, chiral cyclodextrin GC phases have been used, either commercially-available or custom-made versions (e.g., Hillbur et al, 1999). In our experience, these do not work well with chiral alcohols, but give much better resolution of esters.…”

Section: Identification Of Sex Pheromonesmentioning

confidence: 99%

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The Chemical Ecology of Cecidomyiid Midges (Diptera: Cecidomyiidae)

et al. 2012

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The family of cecidomyiid midges (Diptera: Cecidomyiidae) exhibits diversified patterns of life history, behavior, host range, population dynamics and other ecological traits. Those that feed on plants include many important agricultural pests; most cultivated plants are attacked by at least one midge species. Several features of the reproductive biology of cecidomyiid midges point to an important role for chemical communication, with this topic last reviewed comprehensively 12 years ago. Here, we review progress on identification of sex pheromones, chemicals involved in location of host plants, the neurophysiology of reception of volatile chemicals, and application of semiochemicals to management of pest species of cecidomyiid midges that has occurred during the last decade. We hope this review will stimulate and sustain further research in these fields.

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“…Essential compounds that are necessary to release male mating behaviour often occur only in trace amounts. While a mixture of two electrophysically active compounds in the pea-midge Contarinia pisi did not attract males, the addition of 1% 2-acetoxytridecane resulted in a high attractivity (Hillbur et al 1999) of the tripartite mixture; the electrophysical reaction of the males' antenna to the third compound were almost not detectable.…”

Section: Chemistry Of the Male-attracting Compoundsmentioning

confidence: 99%

Two phylogenetically distinct species of sexually deceptive orchids mimic the sex pheromone of their single common pollinator, the cuckoo bumblebee Bombus vestalis

et al. 2011

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Sexually deceptive orchids of the genus Ophrys attract male insects for pollination. Pollinator attraction is achieved by mimicking sex pheromones of virgin females of their pollinators, mostly bee species. In earlier investigations, we showed that the phylogenetically distinct Ophrys species O. chestermanii and O. normanii on Sardinia attract their pollinator, males of the cuckoo bumblebee B. vestalis, with the same bouquets of relatively polar volatile compounds. In this investigation, we studied the sex pheromone of virgin females of B. vestalis with the aim of identifying male-attracting compounds and of comparing them with labellum extracts of the two orchids, which were found to release male-attracting compounds in earlier investigations (Gögler et al. 2009). In bioassays, shock-frozen females, cuticle extracts and polar fractions of cuticle extracts of virgin females stimulated mating behaviour in the males. Using gas chromatography coupled with electroantennography (GC-EAD) and gas chromatography coupled with mass spectrometry (GC-MS), we detected in polar fractions of cuticle extracts of B. vestalis females the same electrophysiologically active compounds as in labellum extracts of both orchid species, including aldehydes, esters, fatty acids and alcohols. Since statistical comparisons of the relative proportions of esters showed strong similarities between virgin females and orchids, our results support the hypotheses that this highly specialized Ophrys-pollinator relationship represents another case of chemical mimicry and that esters play a key role in male attraction.

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Identification of sex pheromone components of the pea midge, Contarinia pisi (Diptera: Cecidomyiidae)

Cited by 25 publications

References 8 publications

Host-associated differences in emergence pattern, reproductive behavior and life history of Asteralobia sasakii (Monzen) (Diptera: Cecidomyiidae) between populations on Ilex crenata and I. integra (Aquifoliaceae)

Host-associated differences in emergence pattern, reproductive behavior and life history of Asteralobia sasakii (Monzen) (Diptera: Cecidomyiidae) between populations on Ilex crenata and I. integra (Aquifoliaceae)

The Chemical Ecology of Cecidomyiid Midges (Diptera: Cecidomyiidae)

Two phylogenetically distinct species of sexually deceptive orchids mimic the sex pheromone of their single common pollinator, the cuckoo bumblebee Bombus vestalis

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