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Electroantennogram, Gas Chromatography, Pheromones, (E,Z)-7,9-Dodecadienyl Acetate, Lobesia botranaA gas chromatographic detector is described which uses an insect antenna as a sensing element and permits the continuous monitoring of pheromones and other compounds with olfactory activity. In high-resolution gas chromatography allowing separations of positional and geometrical isomers, the electroantennographic detector gives precise information on the retention times of active com pounds and permits the analysis of insect pheromones with exceedingly small amounts of material.

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A novel device is described for the quantitative application of chemical stimuli. The device uses ultrasound to disperse a solution of volatile chemicals as an aerosol. A motor-driven syringe controls the rate at which the solution is released from a glass capillary. Vibration of the capillary disperses the released solution into microdroplets that evaporate completely within a few centimeters of the tip. The ratio of chemical stimulus to solvent is maintained until the liquid is dispersed and therefore the release rate of the chemical stimulus can be set and calculated straightforwardly from the dilution factor and the dynamically controllable speed of the syringe plunger. The sprayer permits the delivery of chemical stimulus independent of relative vapor pressures of the components and of environmental factors such as temperature. The sprayer is easy to operate, can be constructed from inexpensive materials, and can be used to emit odor stimuli in the wind tunnel or any other bioassay for pheromones and plant volatiles.

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