Identification of seven telotrisomics of rye (Secale cereale L.)

Melz, G.; Schlegel, R.

doi:10.1007/bf00022929

Cited by 15 publications

(7 citation statements)

References 3 publications

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“…Despite of the promising prospects it needs to be considered that a comprehensive integration of Ddw1 in hybrid breeding programs might narrow the genetic base of rye varieties. In order to counterbalance any potential linkage drag effect, which might be driven by strong selection for a single haplotype variant controlling PH, further GA-sensitive dwarfing genes like Ddw2 (Melz, 1989), Ddw3 (Stojałowski et al, 2015), or Ddw4 (Kantarek et al, 2018) should be evaluated in breeding programs as well. The sequence information on rye GA2ox genes might support the integration of these valuable alleles in elite rye germplasm using precision breeding approaches.…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Profiling and Fine Mapping Identifies a Gibberellin 2-Oxidase Gene Co-segregating With the Dominant Dwarfing Gene Ddw1 in Rye (Secale cereale L.)

et al. 2019

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The gibberellin (GA)-sensitive dwarfing gene Ddw1 provides an opportunity to genetically reduce plant height in rye. Genetic analysis in a population of recombinant inbred lines confirmed a monogenetic dominant inheritance of Ddw1 . Significant phenotypic differences in PH between homo- and heterozygotic genotypes indicate an incomplete dominance of Ddw1 . De novo transcriptome sequencing of Ddw1 mutant as well as tall genotypes resulted in 113,547 contigs with an average length of 318 bp covering 36.18 Mbp rye DNA. A hierarchical cluster analysis based on individual groups of rye homologs of functionally characterized rice genes controlling morphological or physiological traits including plant height, flowering time, and source activity identified the gene expression profile of stems at the begin of heading to most comprehensively mirror effects of Ddw1 . Genome-wide expression profiling identified 186 transcripts differentially expressed between semi-dwarf and tall genotypes in stems. In total, 29 novel markers have been established and mapped to a 27.2 cM segment in the distal part of the long arm of chromosome 5R. Ddw1 could be mapped within a 0.4 cM interval co-segregating with a marker representing the C20-GA2-oxidase gene ScGA2ox12 , that is up-regulated in stems of Ddw1 genotypes. The increased expression of ScGA2ox12 observed in semi-dwarf rye as well as structural alterations in transcript sequences associated with the ScGA2ox12 gene implicate, that Ddw1 is a dominant gain-of-function mutant. Integration of the target interval in the wheat reference genome sequence indicated perfect micro-colinearity between the Ddw1 locus and a 831 kb segment on chromosome 5A, which resides inside of a 11.21 Mb interval carrying the GA-sensitive dwarfing gene Rht12 in wheat. The potential of Ddw1 as a breeder’s option to improve lodging tolerance in rye is discussed.

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Section: Discussionmentioning

confidence: 99%

Gene Expression Profiling and Fine Mapping Identifies a Gibberellin 2-Oxidase Gene Co-segregating With the Dominant Dwarfing Gene Ddw1 in Rye (Secale cereale L.)

et al. 2019

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“…As mentioned in the Introduction, other trisomics than primary trisomics have been isolated in rye, and have been used in genetic experimentation (Melz & Schlegel, 1985). The telocentric 5RS was used to locate the arm in which the genes br and ti are located (Sybenga & Mastenbroek, 1980).…”

Section: Telocentric Tertiary and Other Special Trisomicsmentioning

confidence: 99%

“…The telocentric 5RS was used to locate the arm in which the genes br and ti are located (Sybenga & Mastenbroek, 1980). A telocentric set derived from the 'Esto' primary trisomics (see above) and its use in gene localization has been reported by Lindner et al (1984), Melz & Schlegel (1985) and Melz (1988). In 1987 Zeller et al described a set of telocentric trisomics derived from the primary trisomics of Heines Hellkorn (see above), and their hybridization with an addition set of rye chromosomes to wheat.…”

Section: Telocentric Tertiary and Other Special Trisomicsmentioning

confidence: 99%

Aneuploid and other cytological tester sets in rye

Sybenga

1996

Euphytica

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Cytological tester sets include series of aneuploids (nullisomics, monosomics, trisomics of different types, tetrasomics), series of rearranged chromosomes (translocations, inversions, duplications, deficiencies) and series of chromosomes recognizable by specific microscopically visible markers (C-or other banding, molecular markers). In rye, only a few (mainly tertiary and telocentric) monosomics and no viable nuilisomics have been found. Several sets of primary trisomics and some telocentric sets, usually not fully complete, have been developed, but few are still available for gene localization. A few tertiary trisomics have been derived from translocation heterozygotes. Extensively used are different sets of additions of rye chromosomes to wheat. A relatively widely distributed set of marked chromosomes is the Wageningen translocation tester set, complemented with translocations from different other institutions. A disadvantage of rye translocations is insufficient heterozygote semisterility. Series of otherwise rearranged chromosomes have not been reported. Sets of lines with chromosomes conspicuously differing from the standard C-banding pattern have been obtained. Molecular markers are available for most rye chromosome, but lack of heterozygosity, necessary for classification after in situ hybridization is a restriction for use as cytological testers. In the cases of most translocations, C-banding and in situ molecular markers, each separate plant in a segregating population must be screened cytologically, whereas with aneupioid markers or with translocations having sufficient heterozygote semisterility, analyzing segregations is sufficient. This article has been previously published in EuphyticaVol. 83, pp. 53-61.

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“…The C-banding technique was also successfully applied to trisomic and telotrisomic rye lines (Zeller et al 1977;Melz and Schlegel 1985). In primary trisomic lines one complete chromosome is triplicated.…”

Section: Introductionmentioning

confidence: 99%

“…However, it is well known that Giemsa C-banding techniques permit the identification of individual chromosomes and chromosome segments in cultivated rye. Numerous studies have been carried out to recognize rye chromosomes either in diploid rye or in wheat-rye hybrids (Gill and Kimber 1974;Darvey and Gustafson 1975;Singh and Robbelen 1976;Lelley et al 1978;Schlegel et al 1985;Seal 1982;Seal and Bennett 1981;Sybenga 1983;Gustafson et al 1983;Cermefio and Lacadena 1985;Badaev et al 1985).…”

Section: Introductionmentioning

confidence: 99%

Cytological identification of telotrisomic and double ditelosomic lines in Secale cereale cv. Heines Hellkorn by means of Giemsa C-banding patterns and crosses with wheat–rye addition lines

Zeller¹,

Cermeño²,

Friebe³

1987

Genome

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Seven telotrisomic lines (1RS, 1RL, 2RS, 2RL, 3RS acro, 5RS, and 6RS), two double monotelosomic, and two double ditelosomic lines of Secale cereale cv. Heines Hellkorn were analyzed by means of Giemsa C-banding techniques. In crosses with several wheat–rye chromosome addition lines, the telosomic chromosomes in double ditelosomic lines 1/23 and 3/23 were found to be homologous to chromosomes 1R and 2RL of cv. Imperial rye. The C-banding pattern observed for the telosomes in these lines was similar to that detected in the 1R and 2R telosomics of the corresponding telotrisomic lines. Key words: Secale cereale, telotrisomics, double ditelosomics, C-banding pattern.

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Identification of seven telotrisomics of rye (Secale cereale L.)

Cited by 15 publications

References 3 publications

Gene Expression Profiling and Fine Mapping Identifies a Gibberellin 2-Oxidase Gene Co-segregating With the Dominant Dwarfing Gene Ddw1 in Rye (Secale cereale L.)

Gene Expression Profiling and Fine Mapping Identifies a Gibberellin 2-Oxidase Gene Co-segregating With the Dominant Dwarfing Gene Ddw1 in Rye (Secale cereale L.)

Aneuploid and other cytological tester sets in rye

Cytological identification of telotrisomic and double ditelosomic lines in Secale cereale cv. Heines Hellkorn by means of Giemsa C-banding patterns and crosses with wheat–rye addition lines

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