Cytological identification of telotrisomic and double ditelosomic lines in Secale cereale cv. Heines Hellkorn by means of Giemsa C-banding patterns and crosses with wheat–rye addition lines

Abstract

Seven telotrisomic lines (1RS, 1RL, 2RS, 2RL, 3RS acro, 5RS, and 6RS), two double monotelosomic, and two double ditelosomic lines of Secale cereale cv. Heines Hellkorn were analyzed by means of Giemsa C-banding techniques. In crosses with several wheat–rye chromosome addition lines, the telosomic chromosomes in double ditelosomic lines 1/23 and 3/23 were found to be homologous to chromosomes 1R and 2RL of cv. Imperial rye. The C-banding pattern observed for the telosomes in these lines was similar to that detected in the 1R and 2R telosomics of the corresponding telotrisomic lines. Key words: Secale cereale, telotrisomics, double ditelosomics, C-banding pattern.

Tools and methodologies for cytogenetic studies of plant chromosomes

“…C banding has been used in the identification of aneuploids (Linde Laursen, 1978bZeller et al, 1987), translocations and other structural rearrangements (Gill and Kimber, 1977;Lukas zewski and Gustafson, 1983;Lapitan et al, 1984) and the precise physical mapping of genes (Kota and Dvorak, 1986;Jampates and Dvorak, 1986). C banding analysis of durum timopheevi and durum speltoides hybrids by Chen and Gill (1983) has supported Dvorak's suggestion (1983) that chromosomes 4A and 4B should be reassigned to the B and A genomes, respectively.…”

“…(Morris and Gill, 1987). N banding has also been used to iden tify various types of aneuploids (Singh and Tsuchiya, 198Zb;Zeller et al, 1987), alien addi tion and substitution lines (Islam, 1980), and translocations and deletions (Jewell, 1978). It should be noted that N banding has been attempt ed in T. monococcum by B.S.…”