Tools and methodologies for cytogenetic studies of plant chromosomes

Plant Chromosome Preparations and Staining for Light Microscopic Studies

“…These banding methods are widely used for the identification of individual chromosomes in the karyotype, detection of structural changes in chromosomes, aneuploid identification, chromosome polymorphism and genome analysis of polyploids. Various chromosome banding methods that are reported in usage in plants and animal species are Q-, G-, C-, N-, F-, Hy-, R-and Ag-NOR-banding [15][16][17]. Among these banding methods, Q-and C-banding are widely used in plants.…”

Immunolocalization of chromosome-associated proteins in plants – principles and applications

“…From the development of molecular cytogenetic techniques, understanding the organization of genomes had a large impact on taxonomic and evolutionary studies (Fedak and Kim [2008]; Figueroa and Bass [2010]), in breeding and genetic engineering (Seijo et al [2010]).…”

“…Considerable advances in plant biology have been accompanied by the use of microscopy of higher resolution and improvement of the detection limit of analyses through use of specific antibodies and fluorochromes, that provide an interaction between knowledge of cell biology, classical cytogenetics and molecular cytogenetics. From the development of molecular cytogenetic techniques, understanding the organization of genomes had a large impact on taxonomic and evolutionary studies (Fedak and Kim [ 2008 ]; Figueroa and Bass [ 2010 ]), in breeding and genetic engineering (Seijo et al [ 2010 ]).…”