2001
DOI: 10.1006/bbrc.2001.4589
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Identification of Ser1275 and Ser1309 as Autophosphorylation Sites of the Human Insulin Receptor in Intact Cells

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Cited by 7 publications
(6 citation statements)
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“…However, under these conditions a decrease in Ser phosphorylation of IR was detected, a situation that also favours Tyr phosphorylation of IR, indicating the possibility of an additional extragenic mechanism. It has been proposed that the kinase domain of IR acts as a dual specificity kinase, making phosphorylation of its own Ser and Tyr residues possible [35], as we detected in brown adipocytes. Moreover, rosiglitazone could modulate insulin signalling by acting through phosphatases at the level of the IR.…”
Section: Discussionsupporting
confidence: 63%
“…However, under these conditions a decrease in Ser phosphorylation of IR was detected, a situation that also favours Tyr phosphorylation of IR, indicating the possibility of an additional extragenic mechanism. It has been proposed that the kinase domain of IR acts as a dual specificity kinase, making phosphorylation of its own Ser and Tyr residues possible [35], as we detected in brown adipocytes. Moreover, rosiglitazone could modulate insulin signalling by acting through phosphatases at the level of the IR.…”
Section: Discussionsupporting
confidence: 63%
“…Classically, GRK2 activation leads to phosphorylation of GPCRs on serine/threonine residues and subsequent receptor desensitization (24). Increased Ser phosphorylation of the insulin receptor causes a simultaneous decrease in Tyr phosphorylation of insulin receptor and thus a decrease in receptor activity (25,26). Therefore, we anticipated that the GRK2 depletion would reduce serine phosphorylation of the insulin receptor, enhance Tyr phosphorylation of the insulin receptor, and enhance insulin receptor signaling.…”
Section: Discussionmentioning
confidence: 99%
“…We were also disappointed, but not surprised, that INSR T1161 phosphorylation was not observed in category III because of the low abundance of the INSR (observation of this site in our previous work required two-step isolation and immunoprecipitation of the INSR) (8). The INSR phosphopeptides that were observed in category V are autophosphorylation events and are not associated with any known function (29,30).…”
Section: Investigation Of Hfd-induced Insulin Resistance By Phosphoprmentioning
confidence: 93%