Identification of Schistosoma mansoni eggs and other soil transmitted intestinal parasites in stool using Odongo-Aginya method

Abstract

Objective World Health Organisation recommends Kato-Katz technique for quantitative diagnosis of Schistosoma (S) mansoni and other soil transmitted intestinal parasites in field research. However, the prepared slides in Kato-Katz technique cannot be examined immediately and after 1 to 2 hours, the eggs of parasites with thin cell walls over clear in glycerine and morphology of the eggs are not preserved. These disadvantages are overcome using Odongo-Aginya method as illustrate by S. mansoni eggs and other soil transmitted intestinal parasites in stool in the results. Result The Odongo-Aginya method composed of compound stains of 7.5% nigrosin in 10% formalin and 0.5% eosin yellowin10% formalin mixed 1:1 ratio can be examined immediately. This method illustrated very clearly morphological appearance of S. mansoni and other soil transmitted intestinal parasites. This method showed a clear appearance of intestinal parasites eggs and larvae immediately after preparation and also after six weeks. The prepared slide also maintains the morphological appearance of the eggs even of the hookworm for a long time when the slides are kept in cool dry place. This method is reliable, reproducible, cost effective, easy to learn, quick and safe especially when handling specimens from Human Immunodeficiency virus infected patients.