Identification of Salmonella with the O-1 Bacteriophage

Welkos, Susan L.; Schreiber, Minnie; Baer, Herman

doi:10.1128/aem.28.4.618-622.1974

Cited by 37 publications

(24 citation statements)

References 4 publications

(8 reference statements)

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“…Many bacteriophages are known to be highly specific for their receptors and show little or no interaction with receptors with an even slightly different structure. This specificity forms the basis of numerous phage typing methods for the identification of bacterial species or subspecies (37).…”

mentioning

confidence: 99%

Prevalence of Broad-Host-Range Lytic Bacteriophages of Sphaerotilus natans , Escherichia coli , and Pseudomonas aeruginosa

Jensen

Schrader

Rieland

et al. 1998

Appl Environ Microbiol

249

121

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Two bacteriophage collections were examined with regard to their ability to form plaques on multiple bacterial host species. Nine of 10 phages studied were found to be broad-host-range bacteriophages. These phages fell into two groups. Group 1, the SN series, was isolated from sewage treatment plant samples with Sphaerotilus natansATCC 13338 as a host. The DNAs of these bacteriophages contained modified bases and were insensitive to cleavage by type I and II restriction endonucleases. The efficiency of plating of these bacteriophages was changed only slightly on the alternate host. Group 2, the BHR series, was isolated by a two-host enrichment protocol. These bacteriophages were sensitive to restriction, and their efficiency of plating was dramatically reduced on the alternate host. Our results suggest that a multiple-host enrichment protocol may be more effective for the isolation of broad-host-range bacteriophages by avoiding the selection bias inherent in single-host methods. At least two of the broad-host-range bacteriophages mediated generalized transduction. We suggest that broad-host-range bacteriophages play a key role in phage ecology and gene transfer in nature.

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mentioning

confidence: 99%

Prevalence of Broad-Host-Range Lytic Bacteriophages of Sphaerotilus natans , Escherichia coli , and Pseudomonas aeruginosa

Jensen

Schrader

Rieland

et al. 1998

Appl Environ Microbiol

249

121

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“…Chronic phage can replicate and release their progeny from the host without compromising the viability of their host.Phages can survive outside the host cell but utilization of the host is requiredfor replication 14 . Phage specificity can be used to further characterize bacterial strains through a method called phage typing 15,16 .…”

Section: Phagementioning

confidence: 99%

Using Phage as A Highly Specific Antibiotic Alternative Against Methicillin Resistance Staphylococcus aureus (MRSA)

Rezaei¹,

Nasser²,

Jalilian³

et al. 2014

Biosci., Biotechnol. Res. Asia

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Misuse of antibiotics in humans and animals often leads to the developmentofmultidrug resistance (MDR)bacteria. Resistance can occur within a few years of novel antibiotics being introduced. Lytic bacteriophage (phage) are a kind of virus that undergo a cyclical lifestyle wherein they infect and replicate through the use of a bacterial host cell and cause cell lysis. Phage recognizes specific receptors on their host cell to attach to,insert their DNA and take over their host's molecular machinery. These receptors only exist on the surface of specific bacterial host cells and are often not present on other non-specific bacteria and not especially on the surfaces of eukaryotic cells. The mechanisms by which phage can destroy bacteria are different from antibiotics; phage can lyse MDR resistant bacteria without being affected by hydrolytic enzymes or ribosomal variations that's mean unlike drug resistance mechanism which bacteria can destroy the drug before can inter the bacteria cell, the phage not effected with such enzyme.

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“…It is considered as a potential alternative biocontrol method to inhibit the pathogen [13,14]. Virulent phages cause bacterial host cell lysis and not only function to control bacterial populations but also can be used as indicators of bacterial contamination in fecal samples [15,16] and as a potential tool for identifying specific bacterial strains [17,18]. A multivalent virulent bacteriophage would be a good selection for phage therapy because of its wide host range.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Molecular Characterization and Insight into the Genome Sequence of E. coli Bacteriophage ADB-2 from Poultry Fecal Sample

Dv¹,

Hd²,

Nm³

et al. 2014

Next Generat Sequenc & Applic

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Here we describe the isolation of few E. coli phages specifically ADB-2 and S. typhimurium bacteriophages from poultry fecal sample, its molecular characterization, genome sequencing and functional annotation. Escherichia phage ADB-2 was isolated from a chicken fecal sample. It is a virulent phage and shows effective inhibition of Escherichia coli strains. An antibiogram of the natural host of Escherichia phage ADB-2 showed that the host was sensitive against norfloxacin and gentamicin and that it demonstrated higher resistance against cotrimoxazole and oxytetracycline. The data generated from the genomic library contained 229,781 reads and 45,496,800 nucleotide bases with an average read length of 198 bases. The assembly using Newbler version 2.6 generated a 50,552 bp long single chromosome. The genome annotation and comparative analysis of the genome using Rapid Annotation using Subsystem Technology showed that the phage genome comprised of 46% GC with 76 predicted coding regions and 2 RNA genes. The genome confirmed the presence of functional genes related to phage structure and packaging machinery, phage neck protein, tail structure for host interaction, phage DNA synthesis and host lysis. The complete genomic analysis of phage ADB-2 provides new insights into its characteristics and interactions with the host bacterium Escherichia coli. The complete genome sequence of the Escherichia phage ADB-2 genome can be accessed under the Gen Bank accession number JX912252.1.

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Identification of Salmonella with the O-1 Bacteriophage

Cited by 37 publications

References 4 publications

Prevalence of Broad-Host-Range Lytic Bacteriophages of Sphaerotilus natans , Escherichia coli , and Pseudomonas aeruginosa

Prevalence of Broad-Host-Range Lytic Bacteriophages of Sphaerotilus natans , Escherichia coli , and Pseudomonas aeruginosa

Using Phage as A Highly Specific Antibiotic Alternative Against Methicillin Resistance Staphylococcus aureus (MRSA)

Isolation, Molecular Characterization and Insight into the Genome Sequence of E. coli Bacteriophage ADB-2 from Poultry Fecal Sample

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