Identification of Rickettsia spp., Anaplasma spp., and an Ehrlichia canis-like agent in Rhipicephalus microplus from Southwest and South-Central China

Lu, Miao; Tian, Jingkui; Pan, Xingliang; Qin, Xin-Cheng; Wang, Wen; Chen, Jintao; Guo, Wen‐Ping; Li, Kun

doi:10.1016/j.ttbdis.2021.101884

Cited by 15 publications

(18 citation statements)

References 68 publications

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“…The 16S rRNA sequences of the randomly selected strains ( E. canis Qianxinan8 and E. canis Qianxinan10) had three different nucleotides and they were divided into different clades in the phylogenetic tree. However, their gltA and groEL genes were identical to each other, and they both showed the highest identity to E. canis strains we previously identified in Guangxi Province of Southwest China, which is adjacent to Guizhou Province [ 10 ]. Ehrlichia minasensis was detected in both Qianxinan and Liupanshui, with positive rates of 2.47% (2/81) and 5.56% (7/126) ( Table 1 ).…”

Section: Resultsmentioning

confidence: 94%

“…In China, R. microplus has been recorded in 188 counties [ 11 ]. In a previous study, we identified various Rickettsia , Anaplasma , and Ehrlichia species in R. microplus ticks from Hainan, Yunnan, Guangxi, Sichuan, and Hubei Provinces in China [ 10 , 16 ]. In this study, one Rickettsia , four Anaplasma , and three Ehrlichia species in total were detected and characterized in Guizhou Province.…”

Section: Discussionmentioning

confidence: 99%

“…As an important vector of pathogens, R. microplus harbors a large variety of animal (including human) pathogens such as spotted fever group Rickettsia (SFGR, agents of human spotted fever), Anaplasma phagocytophilum , A. capra , A. marginale (the agent of bovine anaplasmosis), Ehrlichia chaffeensis , E. canis (the agent of canine ehrlichiosis), E. minasensis (the agent of bovine ehrlichiosis), Babesia bigemina , B. bovis (the agent of bovine babesiosis), Borrelia spp. (agents of Lyme disease and relapsing fever), and severe fever with thrombocytopenia syndrome virus (SFTSV) [ 8 , 9 , 10 , 11 ], thus resulting in infections in both humans and domestic animals.…”

Section: Introductionmentioning

confidence: 99%

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Diversity of Rickettsiales in Rhipicephalus microplus Ticks Collected in Domestic Ruminants in Guizhou Province, China

Meng

Gao³

et al. 2022

Pathogens

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Rhipicephalus microplus ticks are vectors for multiple pathogens infecting animals and humans. Although the medical importance of R. microplus has been well-recognized and studied in most areas of China, the occurrence of tick-borne Rickettsiales has seldom been investigated in Guizhou Province, Southwest China. In this study, we collected 276 R. microplus ticks from cattle (209 ticks) and goats (67 ticks) in three locations of Guizhou Province. The Rickettsia, Anaplasma, and Ehrlichia were detected by targeting the 16S rRNA gene and were further characterized by amplifying the key genes. One Rickettsia (Ca. Rickettsia jingxinensis), three Ehrlichia (E. canis, E. minasensis, Ehrlichia sp.), and four Anaplasma (A. capra, A. ovis, A. marginale, Ca. Anaplasma boleense) species were detected, and their gltA and groEL genes were recovered. Candidatus Rickettsia jingxinensis, a spotted fever group of Rickettsia, was detected in a high proportion of the tested ticks (88.89%, 100%, and 100% in ticks from the three locations, respectively), suggesting the possibility that animals may be exposed to this type of Rickettsia. All the 16S, gltA, groEL, and ompA sequences of these strains are 100% identical to strains reported in Ngawa, Sichuan Province. E. minasensis, A. marginale, and Candidatus Anaplasma boleense are known to infect livestock such as cattle. The potential effects on local husbandry should be considered. Notably, E. canis, A. ovis, and A. capra have been reported to infect humans. The relatively high positive rates in Qianxinan (20.99%, 9.88%, and 4.94%, respectively) may indicate the potential risk to local populations. Furthermore, the genetic analysis indicated that the E. minasensis strains in this study may represent a variant or recombinant. Our results indicated the extensive diversity of Rickettsiales in R. microplus ticks from Guizhou Province. The possible occurrence of rickettsiosis, ehrlichiosis, and anaplasmosis in humans and domestic animals in this area should be further considered and investigated.

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Section: Resultsmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Diversity of Rickettsiales in Rhipicephalus microplus Ticks Collected in Domestic Ruminants in Guizhou Province, China

Meng

Gao³

et al. 2022

Pathogens

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“…in R. annulatus collected from donkeys in Beni-Suef, Egypt, inferring that this new potential pathogen spreads among provinces, and R. annulatus is the main vector of this species in Egypt. This new species was detected in other countries such as China in R. microplus [ 54 ], Turkey in Hyalomma excavatum [ 55 ], and Pakistan in H. anatolicum [ 56 ]. Nevertheless, R. annulatus was free from piroplasms.…”

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiological Investigation of Piroplasms and Anaplasmataceae Bacteria in Egyptian Domestic Animals and Associated Ticks

et al. 2022

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Piroplasmosis and anaplasmosis are serious tick-borne diseases (TBDs) that are concerning for the public and animal health. This study aimed to detect the molecular prevalence and epidemiological risk factors of Piroplasma and Anaplasma species in animal hosts and their associated ticks in Egypt. A total of 234 blood samples and 95 adult ticks were collected from animal hosts (112 cattle, 38 sheep, 28 goats, 26 buffaloes, 22 donkeys, and 8 horses) from six provinces of Egypt (AL-Faiyum, AL-Giza, Beni-Suef, Al-Minufia, Al-Beheira, and Matruh). Blood and tick samples were investigated by polymerase chain reaction coupled with sequencing targeting 18S and 16S RNA genes for Piroplasma sp. and anaplasmataceae, respectively. Statistical analysis was conducted on the potential epidemiological factors. Of the 234 animals examined, 54 (23.08%) were positive for pathogens DNA distributed among the six provinces, where 10 (4.27%) were positive for Piroplasma sp., 44 (18.80%) for anaplasmataceae, and 5 (2.14%) were co-infected. Co-infections were observed only in cattle as Theileria annulata and Anaplasma marginale plus Babesia bigemina, A. marginale plus B. bigemina, and T. annulata plus B. bigemina. Piroplasmosis was recorded in cattle, with significant differences between their prevalence in their tick infestation factors. Animal species, age, and tick infestation were the potential risk factors for anaplasmosis. All ticks were free from piroplasms, but they revealed high prevalence rates of 72.63% (69/95) with anaplasmataceae. We identified T. annulata, B. bigemina, and A. marginale in cattle; A. platys in buffaloes; A. marginale and A. ovis in sheep; for the first time, A. ovis in goats; and Ehrlichia sp. in Rhipicephalus annulatus ticks. Our findings confirm the significant prevalence of piroplasmosis and anaplasmosis among subclinical and carrier animals in Egypt, highlighting the importance of the government developing policies to improve animal and public health security.

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“…PCR amplification was performed using a Sensoquest PCR System LabCycler (Göttingen, Germany). The cycling conditions were: 94 °C for 3 min, 35 cycles of 94 °C for 30 s, 55 °C for 1 min, 72 °C for 1 min, and a final extension of 72 °C for 5 min [ 44 ]. After electrophoresis, the amplicons were subjected to sequencing and analyzed by BLASTN alignment.…”

Section: Methodsmentioning

confidence: 99%

Molecular Survey of Vector-Borne Pathogens in Ticks, Sheep Keds, and Domestic Animals from Ngawa, Southwest China

Tian

Zhao

et al. 2022

Pathogens

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Vector-borne pathogens are mainly transmitted by blood-feeding arthropods such as ticks, mosquitoes, fleas, lice, mites, etc. They pose a significant threat to animal and human health due to their worldwide distribution. Although much work has been performed on these pathogens, some neglected areas and undiscovered pathogens are still to be further researched. In this study, ticks (Haemaphysalis qinghaiensis), sheep keds (Melophagus ovinus), and blood samples from yaks and goats were collected in Ngawa Tibetan and Qiang Autonomous Prefecture located on the eastern edge of the Qinghai–Tibet Plateau, Southwest China. Several vector-borne bacterial pathogens were screened and studied. Anaplasma bovis strains representing novel genotypes were detected in ticks (8.83%, 37/419), yak blood samples (45.71%, 64/140), and goat blood samples (58.93%, 33/56). Two spotted fever group (SFG) Rickettsiae, Candidatus Rickettsia jingxinensis, and a novel Rickettsia species named Candidatus Rickettsia hongyuanensis were identified in ticks. Another Rickettsia species closely related to the Rickettsia endosymbiont of Polydesmus complanatus was also detected in ticks. Furthermore, a Coxiella species was detected in ticks (3.34%, 14/419), keds (1.89%, 2/106), and yak blood (0.71%, 1/140). Interestingly, another Coxiella species and a Coxiella-like bacterium were detected in a tick and a goat blood sample, respectively. These results indicate the remarkable diversity of vector-borne pathogens circulating in this area. Further investigations on their pathogenicity to humans and domestic animals are still needed.

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Identification of Rickettsia spp., Anaplasma spp., and an Ehrlichia canis-like agent in Rhipicephalus microplus from Southwest and South-Central China

Cited by 15 publications

References 68 publications

Diversity of Rickettsiales in Rhipicephalus microplus Ticks Collected in Domestic Ruminants in Guizhou Province, China

Diversity of Rickettsiales in Rhipicephalus microplus Ticks Collected in Domestic Ruminants in Guizhou Province, China

Molecular Epidemiological Investigation of Piroplasms and Anaplasmataceae Bacteria in Egyptian Domestic Animals and Associated Ticks

Molecular Survey of Vector-Borne Pathogens in Ticks, Sheep Keds, and Domestic Animals from Ngawa, Southwest China

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