Identification of rice blast resistance genes in the elite hybrid rice restorer line Yahui2115

Shi, Jun; Li, Deqiang; Li, Yan; Li, Xiaoyan; Guo, Xiaoyi; Luo, Yiwan; Lu, Yuangen; Zhang, Qin; Xu, Yong‐Ju; Fan, Jing; Huang, Fu; Wang, Wenming

doi:10.1139/gen-2015-0005

Cited by 12 publications

(12 citation statements)

References 38 publications

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“…Yahui2115 is an elite hybrid restorer line carrying several blast resistance genes and widely used in breeding programs (Shi et al 2015). The mRNA amounts of OsDCL1 increased in LTH at 24 and 48 hpi of M. oryzae (Fig.…”

Section: Osdcl1 Is Involved In Osa-mir162-regulated Rice Blast Resistmentioning

confidence: 99%

Osa-miR162a fine-tunes rice resistance to Magnaporthe oryzae and Yield

Wang

et al. 2020

Rice

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MicroRNAs (miRNAs) play essential roles in rice immunity against Magnaporthe oryzae, the causative agent of rice blast disease. Here we demonstrate that Osa-miR162a fine-tunes rice immunity against M. oryzae and yield traits. Overexpression of Osa-miR162a enhances rice resistance to M. oryzae accompanying enhanced induction of defense-related genes and accumulation of hydrogen peroxide (H 2 O 2). In contrast, blocking Osa-miR162 by overexpressing a target mimic of Osa-miR162a enhances susceptibility to blast fungus associating with compromised induction of defense-related gene expression and H 2 O 2 accumulation. Moreover, the transgenic lines overexpressing Osa-miR162a display decreased seed setting rate resulting in slight reduced yield per plant, whereas the transgenic lines blocking Osa-miR162 show an increased number of grains per panicle, resulting in increased yield per plant. Altered accumulation of Osa-miR162 had a limited impact on the expression of rice Dicer-like 1 (OsDCL1) in these transgenic lines showing normal gross morphology, and silencing of OsDCL1 led to enhanced resistance to blast fungus similar to that caused by overexpression of Osa-miR162a, suggesting the involvement of OsDCL1 in Osa-miR162a-regulated resistance. Together, our results indicate that Osa-miR162a is involved in rice immunity against M. oryzae and fine-tunes resistance and yield.

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Section: Osdcl1 Is Involved In Osa-mir162-regulated Rice Blast Resistmentioning

confidence: 99%

Osa-miR162a fine-tunes rice resistance to Magnaporthe oryzae and Yield

Wang

et al. 2020

Rice

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“…To explore the roles of OsDCL1 in rice resistance, we examined the expression of OsDCL1 in susceptible accession LTH and resistance accessions IRBLKm-Ts and Yahui2115 upon M. oryzae infection, respectively. Yahui2115 is an elite hybrid restorer line carrying several blast resistance genes and widely used in breeding programs (Shi et al 2015). The mRNA amounts of OsDCL1 increased in LTH at 24 and 48 hpi of M. oryzae (Fig.…”

Section: Osdcl1 Is Involved In Osa-mir162-regulated Rice Blast Resistmentioning

confidence: 99%

Osa-miR162a fine-tunes rice resistance to Magnaporthe oryzae and yield

Wang

et al. 2020

Preprint

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MicroRNAs (miRNAs) play essential roles in rice immunity against Magnaporthe oryzae, the causative agent of rice blast disease. Here we demonstrate that Osa-miR162a fine-tunes rice immunity against M. oryzae and yield traits. Overexpression of Osa-miR162a enhances rice resistance to M. oryzae accompanying enhanced induction of defense-related genes and accumulation of hydrogen peroxide (H2O2). In contrast, blocking Osa-miR162 by overexpressing a target mimic of Osa-miR162a enhances susceptibility to blast fungus associating with compromised induction of defense-related gene expression and H2O2 accumulation. Moreover, the transgenic lines overexpressing Osa-miR162a display decreased seed setting rate resulting in slight reduced yield per plant, whereas the transgenic lines blocking Osa-miR162 show an increased number of grains per panicle, resulting in increased yield per plant. Altered accumulation of Osa-miR162 had a limited impact on the expression of rice Dicer-like 1 (OsDCL1) in these transgenic lines showing normal gross morphology, and silencing of OsDCL1 led to enhanced resistance to blast fungus similar to that caused by overexpression of Osa-miR162a, suggesting the involvement of OsDCL1 in Osa-miR162a-regulated resistance. Together, our results indicate that Osa-miR162a is involved in rice immunity against M. oryzae and fine-tunes resistance and yield.

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“…The rice blast susceptible japonica variety of rice, Lijiangxin Tuan Heigu (LTH), which is considered to carry no R genes (Shi et al, 2015), was used as a negative control for rice blast evaluation. PuJiang 6 (PJ6), a cultivar highly susceptible to rice false smut, was used as a negative control for rice false smut evaluation.…”

Section: Plant Materialsmentioning

confidence: 99%

“…To date, more than 100 rice blast resistant genes (R genes) have been mapped, and 23 R genes have been cloned (Fath et al, 2014;Shi et al, 2015;Vasudevan et al, 2015). However, rice false smut R genes have not yet been identified.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Resistance to Rice Blast and False Smut of 18 Varieties of Hybrid Rice in Sichuan Province, China

Li¹

2017

IJAB

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Rice blast and false smut are severe diseases of rice reducing not only rice yield but also affect grain quality. The most effective and economical mean of controlling diseases is to breed disease-resistant rice cultivars. Here, we used 18 hybrid rice varieties for identifying their functional rice blast resistance genes, and for estimating their sensitivities to false smut disease. This experiment was conducted in Sichuan Province, China in 2012-2014. The results showed that the Pi2 and Pik loci have strong rice blast resistance. Rice varieties i.e. CNY445, CNYHZ, and YXY2115 expressed the Pi2, while CFY188 and LY908 expressed Pikm. Varieties R18/2348, CGY2348, GY66, YLY973 and N5Y828 demonstrated strong resistance to rice false smut. No correlation in the resistance between rice blast and false smut was observed among the 18 hybrid rice varieties. These results can provide guidance for rational distribution of hybrid rice varieties.

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Identification of rice blast resistance genes in the elite hybrid rice restorer line Yahui2115

Cited by 12 publications

References 38 publications

Osa-miR162a fine-tunes rice resistance to Magnaporthe oryzae and Yield

Osa-miR162a fine-tunes rice resistance to Magnaporthe oryzae and Yield

Osa-miR162a fine-tunes rice resistance to Magnaporthe oryzae and yield

Analysis of the Resistance to Rice Blast and False Smut of 18 Varieties of Hybrid Rice in Sichuan Province, China

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