Identification of rhodamine 123-positive stem cell subpopulations in canine hepatocellular carcinoma cells

Abstract

Abstract. The majority of cases of chemotherapy for hepatocellular carcinoma (HCC) are not effective in human or veterinary medicine due to resistance against anticancer agents. In human medicine, hepatocellular carcinoma stem cells (HCSCs) were recently identified as cytokeratin 19 (CK19)-, cluster of differentiation (CD)-44-, and CD133-positive. However, there are few previous reports regarding canine HCSC (cHCSC). Additionally, to the best of our knowledge, the chemoresistance against anticancer agents of these cHCSCs has not been investigated. In the present study staining of cHCSCs was performed with rhodamine 123, a low-toxicity fluorescent dye for mitochondria, by flow cytometry. There were two subpopulations in the HCC cell line defined by their higher (Rho

Autofluorescence of NADH is a new biomarker for sorting and characterizing cancer stem cells in human glioma

“…1a, Additional file 1: Figure S1). According to previous reports [26, 27], we defined the highest top 10% intensity as high autofluorescence of NADH (NADH high ) and defined the lowest bottom 10% intensity as low autofluorescence of NADH (NADH low ). Accordingly, we sorted the subpopulations with top 10% and bottom 10% intensity of NADH autofluorescence from GBM1 and LN229 cells (Fig.…”

Quantum mechanical, molecular docking, molecular dynamics, ADMET and antiproliferative activity onTrypanosoma cruzi(Y strain) of chalcone (E)-1-(2-hydroxy-3,4,6-trimethoxyphenyl)-3-(3-nitrophenyl)prop-2-en-1-one derived from a natural product

“…To carry out the simulations, the codes used were: Autodocktools™, 23 AutoDockVina™, 24 Avogadro™ (), 25 Discovery studio visualizer™ viewer, 26 Gabedit 2.5.0, 27 Marvin™ 19.8, 2020, (), 28 Pymol, 29 UCSF Chimera™, 30 Gauss View 5 and Gaussian 09 software. 31 The quantum chemical calculations were carried out using the Density Functional Theory (DFT) method at the B3LYP/6-31G(d,p) computational level in the gas phase.…”

Comparative characteristic of lung cancer stem-like cells generated in vitro under different culture conditions

“…In the first way, 0.2% carboxymethylcellulose (CMC) was used. For this purpose, MOR tumor cell line in the amount of 1.35×10 6 was introduced into T25 culture vials in complete culture medium with the addition of 0.2% CMC (Sigma, USA) [8]. In the second way, a culture plate with a standard adhesive surface was pre-coated with a 2% agarose solution (AppliChem, Germany) prepared on distilled water [9].…”