Autofluorescence of NADH is a new biomarker for sorting and characterizing cancer stem cells in human glioma

Yuan, Ye; Yan, Zexuan; Miao, Jingya; Cai, Ruili; Zhang, Mengsi; Wang, Yanxia; Wang, Lihong; Dang, Weiqi; Wang, Di; Xiang, Dongfang; Wang, Yan; Zhang, Peng; Cui, You‐Hong; Bian, Xiu‐Wu; Ma, Qiang

doi:10.1186/s13287-019-1467-7

Cited by 33 publications

(25 citation statements)

References 43 publications

(44 reference statements)

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“…Although the cause of this bi-modal redox status in AM in the current study is unknown, the importance of this observation is that this can indeed occur in a single animal, and therefore imaging the NAD(H) redox status in AM could serve as an effective tool to more accurately determine the relative activation of macrophages in terms of the pro- and anti- inflammatory status. Once different subpopulations can be established by ORI on the single cell level, it would be interesting to perform gene expression analysis of subpopulations after cell sorting based on optical redox ratio or NADH signals [ 68 ].…”

Section: Discussionmentioning

confidence: 99%

Sex and SP-A2 Dependent NAD(H) Redox Alterations in Mouse Alveolar Macrophages in Response to Ozone Exposure: Potential Implications for COVID-19

Lin

Gandhi

et al. 2020

Antioxidants

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Co-enzyme nicotinamide adenine dinucleotide (NAD(H)) redox plays a key role in macrophage function. Surfactant protein (SP-) A modulates the functions of alveolar macrophages (AM) and ozone (O3) exposure in the presence or absence of SP-A and reduces mouse survival in a sex-dependent manner. It is unclear whether and how NAD(H) redox status plays a role in the innate immune response in a sex-dependent manner. We investigated the NAD(H) redox status of AM from SP-A2 and SP-A knockout (KO) mice in response to O3 or filtered air (control) exposure using optical redox imaging technique. We found: (i) In SP-A2 mice, the redox alteration of AM in response to O3 showed sex-dependence with AM from males being significantly more oxidized and having a higher level of mitochondrial reactive oxygen species than females; (ii) AM from KO mice were more oxidized after O3 exposure and showed no sex differences; (iii) AM from female KO mice were more oxidized than female SP-A2 mice; and (iv) Two distinct subpopulations characterized by size and redox status were observed in a mouse AM sample. In conclusions, the NAD(H) redox balance in AM responds to O3 in a sex-dependent manner and the innate immune molecule, SP-A2, contributes to this observed sex-specific redox response.

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Section: Discussionmentioning

confidence: 99%

Sex and SP-A2 Dependent NAD(H) Redox Alterations in Mouse Alveolar Macrophages in Response to Ozone Exposure: Potential Implications for COVID-19

Lin

Gandhi

et al. 2020

Antioxidants

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“…Because of its autofluorescence, NADH can be quantified by cytometry, 182 and interestingly, the NADH high subpopulation has been related to the CD133 + CSC subpopulation in glioma. 183 …”

Section: Nad + and Cancermentioning

confidence: 99%

NAD+ metabolism, stemness, the immune response, and cancer

Navas

Carnero

2021

Sig Transduct Target Ther

237

218

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NAD+ was discovered during yeast fermentation, and since its discovery, its important roles in redox metabolism, aging, and longevity, the immune system and DNA repair have been highlighted. A deregulation of the NAD+ levels has been associated with metabolic diseases and aging-related diseases, including neurodegeneration, defective immune responses, and cancer. NAD+ acts as a cofactor through its interplay with NADH, playing an essential role in many enzymatic reactions of energy metabolism, such as glycolysis, oxidative phosphorylation, fatty acid oxidation, and the TCA cycle. NAD+ also plays a role in deacetylation by sirtuins and ADP ribosylation during DNA damage/repair by PARP proteins. Finally, different NAD hydrolase proteins also consume NAD+ while converting it into ADP-ribose or its cyclic counterpart. Some of these proteins, such as CD38, seem to be extensively involved in the immune response. Since NAD cannot be taken directly from food, NAD metabolism is essential, and NAMPT is the key enzyme recovering NAD from nicotinamide and generating most of the NAD cellular pools. Because of the complex network of pathways in which NAD+ is essential, the important role of NAD+ and its key generating enzyme, NAMPT, in cancer is understandable. In the present work, we review the role of NAD+ and NAMPT in the ways that they may influence cancer metabolism, the immune system, stemness, aging, and cancer. Finally, we review some ongoing research on therapeutic approaches.

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“…50 Therefore, some researches adopted CD15 and CD133 as isolation hallmarks simultaneously to reduce omission. 51,52 A2B5 is recognized as an isolation hallmark of GSCs. 53 The ability of tumor initiation of A2B5 positive CD133 negative GSCs is stronger than A2B5 negative CD133 negative GSCs.…”

Section: Gscs Isolationmentioning

confidence: 99%

The adaptive transition of glioblastoma stem cells and its implications on treatments

Wang

Zhang

et al. 2021

Sig Transduct Target Ther

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Glioblastoma is the most malignant tumor occurring in the human central nervous system with overall median survival time <14.6 months. Current treatments such as chemotherapy and radiotherapy cannot reach an optimal remission since tumor resistance to therapy remains a challenge. Glioblastoma stem cells are considered to be responsible for tumor resistance in treating glioblastoma. Previous studies reported two subtypes, proneural and mesenchymal, of glioblastoma stem cells manifesting different sensitivity to radiotherapy or chemotherapy. Mesenchymal glioblastoma stem cells, as well as tumor cells generate from which, showed resistance to radiochemotherapies. Besides, two metabolic patterns, glutamine or glucose dependent, of mesenchymal glioblastoma stem cells also manifested different sensitivity to radiochemotherapies. Glutamine dependent mesenchymal glioblastoma stem cells are more sensitive to radiotherapy than glucose-dependent ones. Therefore, the transition between proneural and mesenchymal subtypes, or between glutamine-dependent and glucose-dependent, might lead to tumor resistance to radiochemotherapies. Moreover, neural stem cells were also hypothesized to participate in glioblastoma stem cells mediated tumor resistance to radiochemotherapies. In this review, we summarized the basic characteristics, adaptive transition and implications of glioblastoma stem cells in glioblastoma therapy.

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Autofluorescence of NADH is a new biomarker for sorting and characterizing cancer stem cells in human glioma

Cited by 33 publications

References 43 publications

Sex and SP-A2 Dependent NAD(H) Redox Alterations in Mouse Alveolar Macrophages in Response to Ozone Exposure: Potential Implications for COVID-19

Sex and SP-A2 Dependent NAD(H) Redox Alterations in Mouse Alveolar Macrophages in Response to Ozone Exposure: Potential Implications for COVID-19

NAD+ metabolism, stemness, the immune response, and cancer

The adaptive transition of glioblastoma stem cells and its implications on treatments

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