Identification of Regional Variants of the Rabies Virus within the Canadian Province of Ontario

Nadin-Davis, Susan A.; Casey, G A; Wandeler, A. I.

doi:10.1099/0022-1317-74-5-829

Cited by 68 publications

(38 citation statements)

References 24 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This tendency was also consistent for other groups outside the CosC, such as group IX (35,36). Dog rabies epizootics in the Americas seem to be characterized by the circulation of multiple geographic or temporal lineages that have either become extinct or merged over time (2,3,8,10,21,22,(32)(33)(34). Such a tendency was clearly observed in groups I, II, and VI and was implicit in groups III, IV, V, and VII (2,3,5,8,10,12,21-23,31-33, 35,36).…”

Section: Discussionmentioning

confidence: 50%

“…The disease is independently maintained by several species of mammals within the orders Carnivora and Chiroptera (1). Intraspecies RV transmission maintains the disease within geographically discrete (2,3) areas by distinctive virus variants and lineages, which can be identifi ed either by panels of monoclonal antibodies or by genetic analysis (4,5). Interspecies transmission of rabies often results in ecological bottlenecks and epidemiologic dead ends (1,4,6).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Enzootic Rabies Elimination from Dogs and Reemergence in Wild Terrestrial Carnivores, United States

Velasco-Villa¹,

Reeder²,

Orciari³

et al. 2008

Emerg. Infect. Dis.

134

146

View full text Add to dashboard Cite

To provide molecular and virologic evidence that domestic dog rabies is no longer enzootic to the United States and to identify putative relatives of dog-related rabies viruses (RVs) circulating in other carnivores, we studied RVs associated with recent and historic dog rabies enzootics worldwide. Molecular, phylogenetic, and epizootiologic evidence shows that domestic dog rabies is no longer enzootic to the United States. Nonetheless, our data suggest that independent rabies enzootics are now established in wild terrestrial carnivores (skunks in California and northcentral United States, gray foxes in Texas and Arizona, and mongooses in Puerto Rico), as a consequence of different spillover events from long-term rabies enzootics associated with dogs. These preliminary results highlight the key role of dog RVs and human-dog demographics as operative factors for host shifts and disease reemergence into other important carnivore populations and highlight the need for the elimination of dog-related RVs worldwide.

show abstract

Section: Discussionmentioning

confidence: 50%

mentioning

confidence: 99%

Enzootic Rabies Elimination from Dogs and Reemergence in Wild Terrestrial Carnivores, United States

Velasco-Villa¹,

Reeder²,

Orciari³

et al. 2008

Emerg. Infect. Dis.

134

146

View full text Add to dashboard Cite

show abstract

“…Concomitantly, by reason that the N gene amplified for diagnosis can be applied to genetic analysis, it has been employed in many molecular epidemiological studies of rabies both at global (7,17) and regional (8,11,12) levels. In this study, we partially sequenced two regions of the N gene from nucleotides 71 to 348 and 1,149 to 1,420.…”

Section: Discussionmentioning

confidence: 99%

“…The virus has an unsegmented negative-sense RNA genome with a length of about 12 Kb, containing five cistrons which are arranged in order from 3' to 5' end as N, P (former NS), M, G and L. Grasping the dynamics of the rabies virus in nature is very important for prevention of the disease. To date, molecular epidemiological studies of rabies have been performed actively both at global (7,17,20) and regional levels (2,3,8,11,12,14,18,19). Kissi et al (7) carried out a phylogenetic analysis based on diversity of the nucleoprotein (N) gene of 69 isolates from various parts of the world.…”

mentioning

confidence: 99%

Molecular Epidemiology of Rabies in Thailand

Ito

Sugiyama

Oraveerakul

et al. 1999

Microbiology and Immunology

View full text Add to dashboard Cite

For the purpose of making clear the dynamics of rabies viruses that are prevalent among dogs in Asia, especially Thailand, nucleoprotein (N) genes of isolates derived from Thailand were partially sequenced, and a phylogenetic analysis was performed on the basis of the sequencing data. Firstly, all 27 isolates from Thailand belonged to one group that was distantly related to an isolate from China and was separated into at least six lineages. On the other hand, the isolate from Japan was related to viruses from the Arctic. Secondly, in order to analyze the diversity of the N gene more conveniently, restriction fragment length polymorphism (RFLP) analysis was performed on the N gene of 27 isolates from Thailand. The RFLP analysis could distinguish the lineages of each isolate, and the lineages of additional 34 isolates were deduced by this method. On examination of the geographical distribution of the six lineages, based on the results of phylogenetic and RFLP analyses, it was clear that infection cycles of the rabies virus in Thailand have tended to be maintained endemically.

show abstract

“…All DNA fragments were separated by agarose gel electrophoresis and eluted from the agarose with GeneClean (Bio 101). The RG gene from a red fox isolate (Nadin-Davis et al, 1993) was obtained from S. Nadin-Davis (Canadian Food Inspection Agency, Nepean, Ontario) and modified by removing an ATG sequence immediately upstream of the presumed translation-initiating ATG, and then cloned into plasmid pSV2X3 (Prevec et al, 1990) to produce pDCSVFx (Fig. 1).…”

Section: Methodsmentioning

confidence: 99%

Development and use of a 293 cell line expressing lac repressor for the rescue of recombinant adenoviruses expressing high levels of rabies virus glycoprotein.

Matthews¹,

Cummings²,

Evelegh³

et al. 1999

Journal of General Virology

View full text Add to dashboard Cite

An expression cassette designed for high-level production of rabies virus glycoprotein (RG) could not be rescued into a replication-defective, adenovirus-based vector using standard procedures. To overcome this difficulty, a 293-based cell line, designated 293LAP13, was constructed that contained and expressed a derivative of the lac repressor protein. The lac operator sequence, to which the repressor binds, was incorporated into an expression cassette, containing a promoter and intron, designed for high-level production of RG. Insertion of a single operator sequence immediately downstream of the transcription start site and the use of the 293LAP13 cell line allowed recombinant viruses that could not be isolated with 293 cells to be rescued efficiently. The operator-containing virus reached higher titres in 293LAP13 than in parental 293 cells and also produced plaques more efficiently in 293LAP13 cells. Moreover, in non-complementing human and canine cell lines, adenovirus vectors with a promoter-intron expression cassette expressed RG at much higher levels than vectors lacking the intron. These observations, together with the demonstration that expression of RG by operator-containing vectors was repressed markedly in 293LAP13 cells and that this inhibition was relieved at least partly by IPTG, suggest that the 293LAP13 cell line may be useful for the rescue and propagation of many vectors in which high expression of the desired protein prevents vector rescue in 293 cells.

show abstract

Identification of Regional Variants of the Rabies Virus within the Canadian Province of Ontario

Cited by 68 publications

References 24 publications

Enzootic Rabies Elimination from Dogs and Reemergence in Wild Terrestrial Carnivores, United States

Enzootic Rabies Elimination from Dogs and Reemergence in Wild Terrestrial Carnivores, United States

Molecular Epidemiology of Rabies in Thailand

Development and use of a 293 cell line expressing lac repressor for the rescue of recombinant adenoviruses expressing high levels of rabies virus glycoprotein.

Contact Info

Product

Resources

About