Identification of RAPD markers linked to the ps gene and their usefulness for purity determination of breeding lines and F1 tomato hybrids

The Novel ps and ps-2 Specific Markers for Selection of Functional Male Sterile Tomato Lines in Breeding Programs and Hybrids Seed Production

“…The functional male sterility in tomato controlled by recessive gene ps and ps-2 is the important trait in F 1 hybrids seed production. Identification of functionally male sterile plants is labour -consuming, it is possible only during of flowering time or on based the number of seeds per fruit (Potaczek & Kubicki, 1986;Staniaszek et al, 2000Staniaszek et al, , 2002. Molecular markers associated with genes ps and ps-2 may be helpful for the identification functionally male sterility.…”

“…Molecular markers associated with genes ps and ps-2 may be helpful for the identification functionally male sterility. The RAPD AFLP and CAPS markers were used to identify locus ps and ps-2 (Staniaszek et al, 2000;Li et al, 2006;Gorguet et al, 2009). RAPD is relatively simple and easy molecular technique, highly discriminate for genetic polymorphism in tomato, but they are dominant and poor reproducibility (Jones et al, 1997).…”

“…Traditional methods of F 1 hybrid tomato (Solanum lycopersicum L.) seed production require manual emasculation of the flowers in the early bud stage. Using female lines possessing the trait of functional male sterility controlled by recessive ps and ps-2 genes can reduce the time and cost of such work (Potaczek & Kubicki, 1986;Atanassova, 1999Atanassova, & 2000Staniaszek et al, 2000). Several tomato F 1 hybrids exhibiting functional sterility controlled by the ps gene, have been developed at the Research Institute of Horticulture in Skierniewice, Poland.…”

“…Several tomato F 1 hybrids exhibiting functional sterility controlled by the ps gene, have been developed at the Research Institute of Horticulture in Skierniewice, Poland. They have been introduced into practical applications and commercial production (Staniaszek et al, 2000(Staniaszek et al, & 2002Kozik & Nowakowska, 2007). In Bulgaria, thirteen F 1 hybrids were developed based on functional sterility controlled by the ps-2 gene (Atanassova, 1999).…”

“…These markers were successfully tested in 123 F 2 plants (Sha et al, 2010). Staniaszek et al (2000) described three RAPD markers, OPH04-670, OPAX10-780 and OPW13-1230, linked to the ps locus. OPAX10-780 and OPW13-1230 were specific to the fertile male parent and can be used for distinguishing true hybrids from self-pollination of the female parents.…”