Identification of RAPD markers linked to sex determination in guggal [Commiphora wightii (Arnott.)] Bhandari

Abstract

Decamer RAPD primers were tested on dioeceious and hermaphrodite plants of Commiphora wightii to identify sex-specific molecular markers. Sixty different random decamer primers were screened out of which only three primers were found to be associated with sex expression. A *1,280-bp fragment from the primer OPN06 was found to be present in all the female individuals. Another primer OPN 16 produced a unique *400-bp amplification product in only hermaphrodite individuals. The third marker, OPA20 amplified a *1,140-bp fragment from female and hermaphrodite DNAs, but failed to do so from the male plant DNAs.

<i>Commiphora wightii</i> (Arnott) Bhandari—A Natural Source of Guggulsterone: Facing a High Risk of Extinction in Its Natural Habitat

“…Kalpesh and Mohan [77] analyzed the crude enzyme extracts of 22 accessions of C. wightii and identified two accessions that should be conserved and maintained in the field gene bank. Molecular marker studies using RAPD [78,79] and ISSR [79] primers have been used to find genetic variation among different populations and genotypes of C. wightii. Genetic variation is important because it provides the "raw material" for natural selection.…”

Asexual Morphological Differences in Male and Female Plants of <i>Commiphora </i><i>w</i><i>ightii</i> (Arn.) Bhandari - An Endangered Medicinal Plant

“…Male and female plants can be easily identified on the basis of flowers but difficult to identify them during non flowering season. RAPD molecular markers have been identified to differentiate male and female plants [18] but it is very expensive, time consuming and not very useful to a field researcher. At the same time RAPD markers are also not highly reliable.…”

MARCADORES RAPDs ASOCIADOS A LA EXPRESIÓN DEL SEXO EN Ceratozamia Mexicana Brongniart (Zamiaceae)

“…En los últimos años ha recibido una atención especial el desarrollo de marcadores moleculares, particularmente aquellos basados en la técnica de PCR, para identificar tempranamente el sexo (Samantaray et al, 2010, Prakash y Van Staden, 2006. La amplificación al azar de ADN Polimórfico (RAPD) basada en la reacción en cadena de la polimerasa (PCR), ha sido empleada como marcador de ADN vinculado al fenotipo sexual en diversas especies vegetales como: Phoenix dactylifera L. (Younis et al, 2008), Populus tomentosa Carr.…”

“…La amplificación al azar de ADN Polimórfico (RAPD) basada en la reacción en cadena de la polimerasa (PCR), ha sido empleada como marcador de ADN vinculado al fenotipo sexual en diversas especies vegetales como: Phoenix dactylifera L. (Younis et al, 2008), Populus tomentosa Carr. (Wanwei et al, 2009), Carica papaya L. (Chaves-Bedoya y Nuñes, 2007), Simmondsia chinensis (Link) Schneider (Agrawal et al, 2007) y Commiphora wightii (Arnott) Bhandari (Samantaray et al, 2010).…”

“…The random amplification of Polymorphic DNA (RAPD) based on the chain reaction of the polymerase (PCR) has been employed as a DNA marker linked to the sexual phenotype in diverse plant species, such as Phoenix dactylifera L. (Younis et al, 2008), Populus tomentosa Carr. (Wanwei et al, 2009), Carica papaya L. (Chaves-Bedoya and Nuñes, 2007), Simmondsia chinensis (Link) Schneider (Agrawal et al, 2007) and Commiphora wightii (Arnott) Bhandari (Samantaray et al, 2010).…”