Identification of QTLs for Stripe Rust Resistance in a Recombinant Inbred Line Population

Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating fungal diseases of wheat worldwide. It is essential to discover more sources of stripe rust resistance genes for wheat breeding programs. Specific locus amplified fragment sequencing (SLAF-seq) is a powerful tool for the construction of high-density genetic maps. In this study, a set of 200 recombinant inbred lines (RILs) derived from a cross between wheat cultivars Chuanmai 42 (CH42) and Chuanmai 55 (CH55) was used to construct a high-density genetic map and to identify quantitative trait loci (QTLs) for stripe rust resistance using SLAF-seq technology. A genetic map of 2828.51 cM, including 21 linkage groups, contained 6732 single nucleotide polymorphism markers (SNP). Resistance QTLs were identified on chromosomes 1B, 2A, and 7B; Qyr.saas-7B was derived from CH42, whereas Qyr.saas-1B and Qyr.saas-2A were from CH55. The physical location of Qyr.saas-1B, which explained 6.24–34.22% of the phenotypic variation, overlapped with the resistance gene Yr29. Qyr.saas-7B accounted for up to 20.64% of the phenotypic variation. Qyr.saas-2A, a minor QTL, was found to be a likely new stripe rust resistance locus. A significant additive effect was observed when all three QTLs were combined. The combined resistance genes could be of value in breeding wheat for stripe rust resistance.

Genome-wide association analysis of stripe rust resistance in modern Chinese wheat

“…Several stripe rust QTLs were mapped near QYr.hbaas-7BL , including QYr.nwafu-7BL [ 47 ], Qyr.saas-7B [ 48 ], Qyrsicau-7BL [ 19 ], QYr.nwafu-7BL [ 23 ], QYr.caas-7BL.2 [ 49 ], and a QTL flanked by wPt-4342 and wPt-8921 [ 50 ].…”

Mapping Quantitative Trait Loci for High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat PI 197734 Using a Doubled Haploid Population and Genotyping by Multiplexed Sequencing

“…Considering the small and unstable effect of QYr197734.wgp-2A , no KASP markers were developed for saturating this QTL region. Stripe rust resistance genes Yr32 ( Eriksen et al, 2004 ), Yrxy2 ( Zhou et al, 2011 ), one resistance QTL tagged by SNP marker IWA544 ( Jighly et al, 2015 ), and Qyr.saas-2A ( Yang et al, 2019 ) are located within the QYr197734.wgp-2A region. Yr32 confers race-specific all-stage resistance to some Pst races.…”

Chromosomal Distribution of Genes Conferring Tolerance to Abiotic Stresses Versus That of Genes Controlling Resistance to Biotic Stresses in Plants

“…Among the 11 articles in the Special Issue on plant genes conferring resistance to biotic stresses [3,[25][26][27][28][29][30][31][32][33][34], seven articles reported results from single resistant genes (or QTL) for crops and plant species, including soybean, rice, wheat, Dasypyrum villosum, Aegilops searsii, Capsicum annuum, and Vitis quinquangularis. The other four articles [27,29,30,32] analyzed multiple QTLs or genomic regions for one or more diseases.…”

“…In the Special Issue, "Mapping Abiotic Stress-Tolerance Genes in Plants" of International Journal of Molecular Sciences, 21 papers, including two reviews and 19 research articles, were published . Eleven research articles [3,[25][26][27][28][29][30][31][32][33][34] were published in the Special Issue "Mapping Plant Genes that Confer Resistance to Biotic Stress. "…”