Identification of QTLs for Improvement of Plant Type in Rice (Oryza sativaL.) Using Koshihikari / Kasalath Chromosome Segment Substitution Lines and Backcross Progeny F₂Population

The QTL Analysis of RuBisCO in Flag Leaves and Non-Structural Carbohydrates in Leaf Sheaths of Rice Using Chromosome Segment Substitution Lines and Backcross Progeny F2 Populations

“…The results of this study showed the importance of the combination of CSSLs and their progeny F 2 population to detect QTLs under the comparatively small effects of complex gene interactions as demonstrated by Ebitani et al (2005) and Kanbe et al (2008). We also confirmed that it was possible to conduct genetic analysis of crop physiological traits using a smaller number of CSSLs (39 lines in this study) in comparison to F 2 or BILs, which could shorten the time and labor for analysis.…”

“…When we observed a significant difference (< 0.05 probability level) between Koshihikari and particular CSSL in multiple years, the putative QTL region was assumed to be located in the substituted chromosomal segment in that CSSL (Kanbe et al, 2008).…”

“…For example, 39 Koshihikari/Kasalath CSSLs have a high detection power but low epistatic effect because basically only a partial segment of one chromosome of Koshihikari was substituted with a Kasalath segment. Furthermore, the combination of QTL analysis using CSSLs and backcross progeny population derived from target CSSL holding the QTL of Koshihikari was also recommended in order to obtain the QTLs with high resolution and to estimate the accurate QTL effects (Ebitani et al, 2005;Kanbe et al, 2008).…”

A Quantitative Trait Locus for Chlorophyll Content and its Association with Leaf Photosynthesis in Rice

“…Since no other QTLs were detected in this population, the remaining 68.7% of phenotypic variance may be due to environmental factors, measuring error or false negative QTL with minor effect. Previous studies have also detected QTLs for chlorophyll content in this region (Yue et al 2006;Kanbe et al 2008). The positions of markers flanking these QTLs were similar among these studies, so the QTL detected here may be same as those identified previously.…”

“…Several putative QTLs have been detected for SPAD value or chlorophyll content in rice (Ishimaru et al 2001;Teng et al 2004;Abdelkhalik et al 2005;Yue et al 2006;Kanbe et al 2008), and some of these have been confirmed by mapping in advanced-generation progeny (Kanbe et al 2008). However, none of these QTLs has been precisely mapped as a Mendelian factor or characterized for its contribution to leaf photosynthesis.…”