Development and use of chromosome segment substitution lines as a genetic resource for crop improvement

Balakrishnan, Divya; Malathi, S.; Mesapogu, Sukumar; Neelamraju, Sarla

doi:10.1007/s00122-018-3219-y

Cited by 85 publications

(90 citation statements)

References 247 publications

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“…IL populations have demonstrated to be a useful and powerful genetic resource for the identification of QTLs in several crops [65]. The present work provides a first phenotypic evaluation involving 17 agronomic traits of the first set of eggplant ILs [16].…”

Section: Discussionmentioning

confidence: 94%

Performance of a Set of Eggplant (Solanum melongena) Lines With Introgressions From Its Wild Relative S. incanum Under Open Field and Screenhouse Conditions and Detection of QTLs

et al. 2020

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Introgression lines (ILs) of eggplant (Solanum melongena) represent a resource of high value for breeding and the genetic analysis of important traits. We have conducted a phenotypic evaluation in two environments (open field and screenhouse) of 16 ILs from the first set of eggplant ILs developed so far. Each of the ILs carries a single marker-defined chromosomal segment from the wild eggplant relative S. incanum (accession MM577) in the genetic background of S. melongena (accession AN-S-26). Seventeen agronomic traits were scored to test the performance of ILs compared to the recurrent parent and of identifying QTLs for the investigated traits. Significant morphological differences were found between parents, and the hybrid was heterotic for vigour-related traits. Despite the presence of large introgressed fragments from a wild exotic parent, individual ILs did not display differences with respect to the recipient parent for most traits, although significant genotype × environment interaction (G × E ) was detected for most traits. Heritability values for the agronomic traits were generally low to moderate. A total of ten stable QTLs scattered across seven chromosomes was detected. For five QTLs, the S. incanum introgression was associated with higher mean values for plant- and flower-related traits, including vigour prickliness and stigma length. For one flower- and four fruit-related-trait QTLs, including flower peduncle and fruit pedicel lengths and fruit weight, the S. incanum introgression was associated with lower mean values for fruit-related traits. Evidence of synteny to other previously reported in eggplant populations was found for three of the fruit-related QTLs. The other seven stable QTLs are new, demonstrating that eggplant ILs are of great interest for eggplant breeding under different environments.

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Section: Discussionmentioning

confidence: 94%

Performance of a Set of Eggplant (Solanum melongena) Lines With Introgressions From Its Wild Relative S. incanum Under Open Field and Screenhouse Conditions and Detection of QTLs

et al. 2020

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“…As the same set of CSSLs has the same or a similar genetic background but differs only in a specific genetic region on one chromosome (thus eliminating the influence of complex genetic backgrounds), CSSLs are ideal materials for studying quantitative traits and QTL mapping in crops. The construction and utilization of CSSLs have been widely reported in tomato, rice, maize and other crops (Balakrishnan et al 2019;Bouchez et al 2002;Monforte and Tanksley 2000;Okada et al 2018;Qi et al 2013;Qiu et al 2017). In cotton, chromosome substitution lines (CSLs) were first constructed, in which a pair of chromosomes or chromosome arms of Upland TM-1 (the recipient parent) were replaced by those of 3-79 (the donor parent, G. barbadense) (Stelly et al 2005).…”

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confidence: 99%

Examining two sets of introgression lines across multiple environments reveals background-independent and stably expressed quantitative trait loci of fiber quality in cotton

et al. 2020

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Key message Background-independent (BI) and stably expressed (SE) quantitative trait loci (QTLs) were identified using two sets of introgression lines across multiple environments. Genetic background more greatly affected fiber quality traits than environmental factors. Sixty-one SE-QTLs, including two BI-QTLs, were novel and 48 SE-QTLs, including seven BI-QTLs, were previously reported. Abstract Cotton fiber quality traits are controlled by QTLs and are susceptible to environmental influence. Fiber quality improvement is an essential goal in cotton breeding but is hindered by limited knowledge of the genetic basis of fiber quality traits. In this study, two sets of introgression lines of Gossypium hirsutum × G. barbadense were used to dissect the QTL stability of three fiber quality traits (fiber length, strength and micronaire) across environments using 551 simple sequence repeat markers selected from our high-density genetic map. A total of 76 and 120 QTLs were detected in the CCRI36 and CCRI45 backgrounds, respectively. Nine BI-QTLs were found, and 78 (41.71%) of the detected QTLs were reported previously. Thirty-nine and 79 QTLs were SE-QTLs in at least two environments in the CCRI36 and CCRI45 backgrounds, respectively. Forty-eight SE-QTLs, including seven BI-QTLs, were confirmed in previous reports, and 61 SE-QTLs, including two BI-QTLs, were considered novel. These results indicate that genetic background more strongly impacts on fiber quality traits than environmental factors. Twenty-three clusters with BI-and/or SE-QTLs were identified, 19 of which harbored favorable alleles from G. barbadense for two or three fiber quality traits. This study is the first report using two sets of introgression lines to identify fiber quality QTLs across environments in cotton, providing insights into the effect of genetic backgrounds and environments on the QTL expression of fiber quality and important information for the genetic basis underlying fiber quality traits toward QTL cloning and molecular breeding.

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“…One approach to integration of G. barbadense into G. hirsutum utilized the development of chromosome segmental introgression lines, whereby genetic markers were used to track introgression of chromosome segments from G. barbadense (Wang et al, 2011, 2012). A review of chromosome segment substitution lines in several crops was reported by Balakrishnan et al (2019). In our research, we have been successful with a different approach to introgression of genes from these three tetraploid species for improvement of upland cotton.…”

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confidence: 85%

Registration of RMBHMTUP‐C4, a Random‐Mated Cotton Population Containing Alleles from Four Gossypium Species

Jenkins

McCarty

Hayes

et al. 2019

J of Plant Registrations

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The genetic base for upland cotton (Gossypium hirsutum L.) breeding needs more diversity. Use of Gossypium wild species is of interest to breeders, but interspecific crosses between G. hirsutum and wild tetraploid species or the cultivated tetraploid species G. barbadense L. have not been widely used due to the extreme difficulty of extracting elite lines in later generations. Following a nonconventional breeding approach, we used 32 chromosome substitution lines to capture allelic diversity from three donor tetraploid Gossypium species and combined that with the intraspecific allelic diversity among five upland cultivars. By random-mating the 32 F 1 hybrids and subsequent generations for five cycles, the intra-and interspecific diversity from four Gossypium AD-genome species was combined in a new random-mated population dubbed Random Mated Barbadense, Hirsutum, Mustelinum, Tomentosum, Upland Population (RMBHMTUP-C4; Reg. no. GP-1061, PI 690471). This unique population should facilitate efforts to diversify the genetic base for upland cotton breeding. Despite its allelic diversity, this population has average phenotypic and fiber characteristics much like an upland cultivar. During development, we did not observe breeding problems normally observed in conventional interspecific crosses. This should add to the usefulness of this population for introgression of alleles from these tetraploid species. We harvested about 2209 bolls each cycle of random mating (11,045 bolls). Assuming 25 seed per boll, this equates to 276,125 hand-crossed seed, each from union of two genetically unique gametes.

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Development and use of chromosome segment substitution lines as a genetic resource for crop improvement

Cited by 85 publications

References 247 publications

Performance of a Set of Eggplant (Solanum melongena) Lines With Introgressions From Its Wild Relative S. incanum Under Open Field and Screenhouse Conditions and Detection of QTLs

Performance of a Set of Eggplant (Solanum melongena) Lines With Introgressions From Its Wild Relative S. incanum Under Open Field and Screenhouse Conditions and Detection of QTLs

Examining two sets of introgression lines across multiple environments reveals background-independent and stably expressed quantitative trait loci of fiber quality in cotton

Registration of RMBHMTUP‐C4, a Random‐Mated Cotton Population Containing Alleles from Four Gossypium Species

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