Identification of QTLs associated with salinity tolerance at late growth stage in barley

Xue, Dawei; Huang, Ya; Zhang, Xiaoqi; Wei, Kang; Westcott, Sharon; Li, Chengdao; Chen, Mingcan; Zhang, Guoping; Lance, Reg

doi:10.1007/s10681-009-9919-2

Cited by 95 publications

(74 citation statements)

References 42 publications

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“…The third QTL linked with germination stage tolerance is mapped on chromosome 5H. This QTL is slightly distant from potassium concentration linked QTL reported previously [2] but appears to be very closely located with seed dormancy linked QTL on chromosome 5HC in a population generated from Stirling and Harrington barley varieties [19]. The chromosome 5H QTL may be the same locus associated with seed dormancy in these studies.…”

Section: Discussionsupporting

confidence: 49%

“…However, QTL identified on chromosome 3H at 192.8 cM distance linked with fresh weight per plant in seedling emergence stage salinity stress and at 176.5 cM distance linked with biomass yield in seedling emergence and leaf expansion stages salinity stress is located at different chromosomal region from region linked with sd1/ denso gene in the Baudin x Ac Metcalfe barley DH population at about 55 cM distance [24]. The QTL in the Baudin x Ac Metcalfe DH population is in a very close chromosomal region to two QTL linked with plant height at maturity reported previously in the CM72 x Gairdner DH population in salinity stressed and non-stressed conditions [2]. A semi-dwarf 1 ( sdw1 ) gene on chromosome 3HL control plant height in barley and sequencing of its different alleles locus points to HvGA20ox2 as the functional gene responsible for the phenotype [24, 25].…”

Section: Discussionmentioning

confidence: 98%

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Early growth stages salinity stress tolerance in CM72 x Gairdner doubled haploid barley population

et al. 2017

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A doubled haploid (DH) population of barley (Hordeum vulgare L.) generated from salinity tolerant genotype CM72 and salinity sensitive variety Gairdner was studied for salinity stress tolerance at germination, seedling emergence and first leaf full expansion growth stages. Germination study was conducted with deionized water, 150 mM and 300 mM NaCl treatments. Seedling stage salinity tolerance was conducted with three treatments: control, 150 mM NaCl added at seedling emergence and first leaf full expansion growth stages. Results from this study revealed transgressive phenotypic segregations for germination percentage and biomass at seedling stage. Twelve QTL were identified on chromosomes 2H–6H each explaining 10–25% of the phenotypic variations. A QTL located at 176.5 cM on chromosome 3H was linked with fresh weight per plant and dry weight per plant in salinity stress induced at first leaf full expansion growth stage, and dry weight per plant in salinity stress induced at seedling emergence. A stable QTL for germination at both 150 and 300 mM salinity stress was mapped on chromosome 2H but distantly located from a QTL linked with seedling stage salinity stress tolerance. QTL, associated markers and genotypes identified in this study play important roles in developing salinity stress tolerant barley varieties.

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Section: Discussionsupporting

confidence: 49%

Section: Discussionmentioning

confidence: 98%

Early growth stages salinity stress tolerance in CM72 x Gairdner doubled haploid barley population

et al. 2017

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“…In barley, many QTLs associated with salt tolerance have been identified [21, 22]. A single locus controlling salt tolerance, HvNax3 , was identified on the short arm of chromosome 7H from a wild barley accession CPI-71284-48, which controls sodium (Na) accumulation in shoots under salt stress [23].…”

Section: Introductionmentioning

confidence: 99%

Multi-omics analysis reveals molecular mechanisms of shoot adaption to salt stress in Tibetan wild barley

Shen

Dai

et al. 2016

BMC Genomics

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BackgroundTibetan wild barley (Hordeum spontaneum L.) has been confirmed to contain elite accessions in tolerance to abiotic stresses, including salinity. However, molecular mechanisms underlying genotypic difference of salt tolerance in wild barley are unknown.ResultsIn this study, two Tibetan wild barley accessions (XZ26 and XZ169), differing greatly in salt tolerance, were used to determine changes of ionomic, metabolomic and proteomic profiles in the shoots exposed to salt stress at seedling stage. Compared with XZ169, XZ26 showed better shoot growth and less Na accumulation after 7 days treatments. Salt stress caused significant reduction in concentrations of sucrose and metabolites involved in glycolysis pathway in XZ169, and elevated level of tricarboxylic acid (TCA) cycle, as reflected by up-accumulation of citric acid, aconitic acid and succinic acid, especially under high salinity, but not in XZ26. Correspondingly, proteomic analysis further proved the findings from the metabolomic study.ConclusionXZ26 maintained a lower Na concentration in the shoots and developed superior shoot adaptive strategies to salt stress. The current result provides possible utilization of Tibetan wild barley in developing barley cultivars for salt tolerance.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3242-9) contains supplementary material, which is available to authorized users.

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“…Furthermore, primer Bmag0125 on chromosome 2H with PIC value 0.567 (Table 3), these markers were sufficient to differentiate all of the genotypes since they generated a high number of allele with high PIC values. The results indicated that this region of chromosome 4H is important for salt tolerance in barley it may be assumed that there is a QTL cluster for salt tolerance in the region of chromosome 4H, and thus the region may be used as an important target for improving salt tolerance of barley Xue et al (2009). The PIC value (0.38) obtained by Pillen et al (2000), who used 22 microsatellite markers and a set of 28 mainly German barley cultivars and two wild forms.…”

Section: Discussionmentioning

confidence: 96%

Genetic diversity and association analysis among Egyptian barley (Hordeum vulgare L.) genotypes with different adaptations to saline conditions analyzed by SSR markers

et al. 2016

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The success of salt tolerance breeding programs employing traditional screening and selection has been limited in the past decades. This study was designed to characterize the genetic diversity within a subset of barley germplasm using microsatellite markers under different adaptations to salt conditions. Twenty-five microsatellites (SSR) representative of the barley genome, were used in 30 barley accessions and cultivars. A total of 59 alleles were generated, the mean number of alleles per locus was 2.4 and the PIC was 0.22. The four genomic Locus Bmac0030, Bmag0125, EBmac0701 and EBmac0871 were sufficient to differentiate the diversity of all genotypes since they generated a high number of allele with high PIC values. Cluster analysis UPGMA based on SSRs data clearly the most differentiate genotypes according to their Salinity tolerance and the PCA recorded 58.8 % of the total variance. Five markers pairs were found to be associated with salinity and showed linkage disequilibrium with r 2 values higher than 0.05. Association analysis along with specific significant alleles of grain yield showed a significant association of the marker Bmag749 (2H, 176 bp) with salinity. Our results shown a small sample and limited markers were used in this study and the results need to be confirmed using linkage mapping with a large association population. However, the results are credible because many of the loci that were identified were associated with traits that were common with previous reports of linkage or association mapping. These will be useful for molecular marker assist selection and molecular design breeding.

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Identification of QTLs associated with salinity tolerance at late growth stage in barley

Cited by 95 publications

References 42 publications

Early growth stages salinity stress tolerance in CM72 x Gairdner doubled haploid barley population

Early growth stages salinity stress tolerance in CM72 x Gairdner doubled haploid barley population

Multi-omics analysis reveals molecular mechanisms of shoot adaption to salt stress in Tibetan wild barley

Genetic diversity and association analysis among Egyptian barley (Hordeum vulgare L.) genotypes with different adaptations to saline conditions analyzed by SSR markers

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