Identification of putative muscarinic acetylcholine receptor genes in Bactrocera dorsalis and functional analysis of Bdor-mAChR-B

Li, Jianfang; Zhang, Xiaoyu; Bai, Xue; Su, Hong-ai; Lu, Yongyue; Qi, Yixin

doi:10.1016/j.ibmb.2021.103657

Cited by 12 publications

(12 citation statements)

References 37 publications

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“…46,69 Previous study indicated that mAChR is involved in the development, locomotion and mating behavior in B. dorsalis. 47 In D. melanogaster, the inositol 1,4,5-trisphosphate receptor (IP 3 R) regulates pupariate through mAChR. 70 Our results indicate ACh activates A-mAChR on the membrane of oocytes through the PLC-IP 3 pathway, and this pathway plays an important role in vitellogenin endocytosis (data not shown).…”

Section: Discussionmentioning

confidence: 79%

“…43,45 However, there is no insecticide that targets insect mAChRs to date. 46,47 Our previous research showed the presence of A-mAChR protein on the oocyte membrane of M. separata, which indicates a possible non- neuronal function of A-mAChR in the reproductive system. 32 However, nothing is known about the source of ACh and the main components of the cholinergic system in the ovary.…”

Section: Discussionmentioning

confidence: 95%

“…They act on ACh synthesis (organophosphates and carbamates on AChE), uptake (spiroindolines on VAChT) or binding receptors (neonicotinoids, spinosyns and nereistoxin analogues on nAChRs) 43,45 . However, there is no insecticide that targets insect mAChRs to date 46,47 . Our previous research showed the presence of A‐mAChR protein on the oocyte membrane of M. separata , which indicates a possible non‐neuronal function of A‐mAChR in the reproductive system 32 .…”

Section: Discussionmentioning

confidence: 99%

“…As we know, insect mAChRs provide continuing fascination and challenge to discover new chemicals with no target site cross‐resistance against known insecticides 46,69 . Previous study indicated that mAChR is involved in the development, locomotion and mating behavior in B. dorsalis 47 . In D. melanogaster , the inositol 1,4,5‐trisphosphate receptor (IP 3 R) regulates pupariate through mAChR 70 .…”

Section: Discussionmentioning

confidence: 99%

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Comparative transcriptome analysis reveals the non‐neuronal cholinergic system in the ovary of the oriental armyworm, Mythimna separata Walker (Lepidoptera: Noctuidae)

Tian

Guo

et al. 2022

Pest Management Science

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BACKGROUND Acetylcholine (ACh), as a classical neurotransmitter, plays great roles in the nervous system. There is increasing evidence of its non‐neuronal roles in regulating basic cell functions in vertebrates. However, knowledge about the non‐neuronal cholinergic system in insects is scarce. RESULTS A comparative transcriptome analysis was performed to investigate differences in the key molecular components of the cholinergic system between the head and ovary. The results showed that expression levels of most cholinergic system‐related genes were higher in the head than in the ovary, and some cholinergic components were absent in the ovary. ACh contents ranged from 0.1 to 1.3 μg mg−1 of wet weight during the development of the ovary, and weak acetylcholinesterase activity was also detected. Moreover, the ovary has a capacity for ACh synthesis. Bromoacetylcarnitine (BrACar), a specific carnitine acetyltransferase (CarAT) inhibitor, greatly inhibits ACh synthesis by 83.83% in ovary homogenates, but bromoacetylcholine (BrACh), a specific choline acetyltransferase (ChAT) inhibitor, has no effect on ACh synthesis in the ovary. These findings indicate that non‐neuronal ACh in the ovary is only catalyzed by CarAT. CONCLUSION This study reveals the existence of the non‐neuronal cholinergic system in the ovary of M. separata, whose synthesis and release mechanisms are different from those of the head. These results provide novel insights into the non‐neuronal cholinergic system in insects, and will be valuable in the discovery of new target genes and the future development of green pest control. © 2022 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Comparative transcriptome analysis reveals the non‐neuronal cholinergic system in the ovary of the oriental armyworm, Mythimna separata Walker (Lepidoptera: Noctuidae)

Tian

Guo

et al. 2022

Pest Management Science

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“…The maintenance of B. dorsalis requires good laboratory conditions and suitable rearing and containment settings (Ekesi et al ., 2007). The ideal climatic conditions for maintaining colonies generally vary between a temperature of 26°C and 28°C, a humidity level between 75% and 80% and photoperiods of 14h and 10h in light and darkness respectively (Ali et al , 2017;Dong et al , 2017;Wei et al , 2018;Zhang et al , 2020;Chen et al , 2021;Li et al , 2022). The optimal artificial food for adult flies is a mixture of yeast extract, sugar, honey, agar and water in proportions of 2.5%, 7.5%, 2.5%, 0.5% and 87% respectively (Aliet al , 2017;Dong et al , 2017;Sohail et al , 2019;Zheng et al , 2019).…”

Section: Breeding and Ovipositing Stimulation Of Bactrocera Dorsalismentioning

confidence: 99%

Developing genetic tools to control the Oriental Fruit Fly Bactrocera dorsalis [Diptera: Tephritidae]: potential strategies and molecular tools

TRAORE¹,

Galizi²,

Kientega³

et al. 2023

Preprint

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Lethal and sublethal effects of broflanilide on four tephritid pests (Diptera: Tephritidae)

Cai

et al. 2023

Pest Management Science

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BACKGROUND Fruit flies are internationally important quarantine or invasive pests of many fruits and vegetables and can cause serious economic losses. Long‐term reliance on insecticides for controlling these pests has led to increasing resistance to multiple insecticides; hence, a new agent is needed. In this study, the acute toxicity and sublethal effects of the novel insecticide broflanilide on four adult fruit fly species, Bactrocera dorsalis, Bactrocera cucurbitae, Bactrocera tau, and Bactrocera correcta, were evaluated. RESULTS Broflanilide was effective against B. dorsalis and B. correcta, with lethal concentration values (amount required to kill 50% of animals; LC50) of 0.390 and 1.716 mg/L. However, for B. cucurbitae (19.673 mg/L) and B. tau (24.373 mg/L), the LC50 was 50–60 times higher than that of B. dorsalis. The survival rates of B. correcta and B. cucurbitae were significantly lower under LC50 treatment than those of the control (corrected for mortality rate). Sublethal concentrations of broflanilide stimulated fecundity in all species except B. tau. The hatching rate at LC50 was significantly lower for B. correcta and B. tau compared with the control and even more so for B. correcta, which was zero. CONCLUSION Broflanilide is potentially an effective insecticide for controlling B. dorsalis and B. correcta. However, the variation in toxicity of broflanilide to the four fruit flies suggests that species variation needs to be carefully considered. Our results highlight the importance of clarifying the sublethal effects of insecticides on target insects to ensure the comprehensive evaluation and rational use of insecticides. © 2023 Society of Chemical Industry.

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Identification of putative muscarinic acetylcholine receptor genes in Bactrocera dorsalis and functional analysis of Bdor-mAChR-B

Cited by 12 publications

References 37 publications

Comparative transcriptome analysis reveals the non‐neuronal cholinergic system in the ovary of the oriental armyworm, Mythimna separata Walker (Lepidoptera: Noctuidae)

Comparative transcriptome analysis reveals the non‐neuronal cholinergic system in the ovary of the oriental armyworm, Mythimna separata Walker (Lepidoptera: Noctuidae)

Developing genetic tools to control the Oriental Fruit Fly Bactrocera dorsalis [Diptera: Tephritidae]: potential strategies and molecular tools

Lethal and sublethal effects of broflanilide on four tephritid pests (Diptera: Tephritidae)

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