Lethal and sublethal effects of broflanilide on four tephritid pests (Diptera: Tephritidae)

Abstract

BACKGROUND Fruit flies are internationally important quarantine or invasive pests of many fruits and vegetables and can cause serious economic losses. Long‐term reliance on insecticides for controlling these pests has led to increasing resistance to multiple insecticides; hence, a new agent is needed. In this study, the acute toxicity and sublethal effects of the novel insecticide broflanilide on four adult fruit fly species, Bactrocera dorsalis, Bactrocera cucurbitae, Bactrocera tau, and Bactrocera correcta, were evaluated. RESULTS Broflanilide was effective against B. dorsalis and B. correcta, with lethal concentration values (amount required to kill 50% of animals; LC50) of 0.390 and 1.716 mg/L. However, for B. cucurbitae (19.673 mg/L) and B. tau (24.373 mg/L), the LC50 was 50–60 times higher than that of B. dorsalis. The survival rates of B. correcta and B. cucurbitae were significantly lower under LC50 treatment than those of the control (corrected for mortality rate). Sublethal concentrations of broflanilide stimulated fecundity in all species except B. tau. The hatching rate at LC50 was significantly lower for B. correcta and B. tau compared with the control and even more so for B. correcta, which was zero. CONCLUSION Broflanilide is potentially an effective insecticide for controlling B. dorsalis and B. correcta. However, the variation in toxicity of broflanilide to the four fruit flies suggests that species variation needs to be carefully considered. Our results highlight the importance of clarifying the sublethal effects of insecticides on target insects to ensure the comprehensive evaluation and rational use of insecticides. © 2023 Society of Chemical Industry.

Toxicity of Beauveria bassiana to Bactrocera dorsalis and effects on its natural predators

“…The diet described by Jin et al (2011b) contains corn gain, banana, sodium benzoate, yeast, sucrose, winding paper, hydrochloric acid, and water. Different B. dorsalis life stages were cultured in the laboratory (temperature: 26 ± 1°C, relative humidity [RH]: 60–70%, and 14:10-h light:dark photoperiod) ( Li et al, 2023 ). Two natural enemies of B. dorsalis , the predator Amblyseius cucumeris (Mesostigmata: Phytoseiidae) and the parasitoid Anastatus japonicus (Hymenoptera: Eupelmidae), were provided by the Biological Control Group, Institute of Plant Protection, Guangdong Academy of Agricultural Sciences.…”

Lethal, Sublethal, and Offspring Effects of Fluralaner and Dinotefuran on Three Species of Bactrocera Fruit Flies

“…The three fruit fly species used in this study ( B. dorsalis , B. cucurbitae , and B. tau ) were susceptible strains that had been cultured in a laboratory for over 60 generations [ 3 ]. The larvae and adults were reared on artificial diets [ 28 , 29 ].…”

“…Due to differences in the oviposition habits between the fruit fly species, oviposition cups with orange juice were used to collect B. dorsalis or eggs, and with pumpkin flesh to collect B. cucurbitae and B. tau eggs; simultaneously, the oviposition quantity of each species was recorded. Additionally, the egg-hatching rate, female fecundity, survival rate (male and female), and F1 sex ratio (proportion of females = Σ♀ ÷ Σ (♀ + ♂)) were observed after each treatment to assess the sublethal effects of both agents on all three species of fruit flies [ 3 ]. Population parameters were calculated according to the protocol described by Zhang et al [ 31 ].…”

“…Bactrocera dorsalis (Hendel), Bactrocera cucurbitae (Coquillett), and Bactrocera tau (Walker) are important invasive fruit fly pests of international concern that can cause significant economic crop damage [ 1 , 2 , 3 , 4 , 5 ]. In China, these three species are widely distributed in regions like Taiwan, Fujian, and Guangdong, causing annual outbreaks and proving difficult to control.…”

“…Additionally, the sublethal effects of the neonicotinoid insecticide thiacloprid may affect the learning and memory behaviors of Apis mellifera L. [ 26 ]. Meanwhile, lethal and sublethal concentrations of cyantraniliprole (bisamide) affect the mating performance of B. dorsalis adults [ 27 ], whereas broflanilide—a meta-diamide insecticide—at its lethal concentration 50 (LC 50 ) affects the hatchability of B. cucurbitae and B. tau [ 3 ].…”