2017
DOI: 10.1186/s13075-017-1228-x
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Identification of potential saliva and tear biomarkers in primary Sjögren’s syndrome, utilising the extraction of extracellular vesicles and proteomics analysis

Abstract: BackgroundThere is a long-lasting need for non-invasive, more accurate diagnostic techniques when evaluating primary Sjögren’s syndrome (pSS) patients. Incorporation of additional diagnostics involving screening for disease-specific biomarkers in biological fluid is a promising concept that requires further investigation. In the current study we aimed to explore novel disease biomarkers in saliva and tears from pSS patients.MethodsLiquid chromatography-mass spectrometry (LC-MS) was performed on stimulated whol… Show more

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Cited by 181 publications
(186 citation statements)
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“…There is still a great need for further research in order to establish a universal, reliable, non-invasive method with high sensitivity and specificity for each subgroup of this syndrome [34]. Recently, metabolomics analyses to identify salivary and lacrimal biomarkers have been attempted using mass spectroscopy with liquid or gas chromatography [35,36]. To our knowledge, NMR has not yet been employed for further exploration of the biomarkers' potential in the diagnosis and monitoring of Sjögren's syndrome.…”
Section: Future Application On Sjögren's Syndromementioning
confidence: 99%
“…There is still a great need for further research in order to establish a universal, reliable, non-invasive method with high sensitivity and specificity for each subgroup of this syndrome [34]. Recently, metabolomics analyses to identify salivary and lacrimal biomarkers have been attempted using mass spectroscopy with liquid or gas chromatography [35,36]. To our knowledge, NMR has not yet been employed for further exploration of the biomarkers' potential in the diagnosis and monitoring of Sjögren's syndrome.…”
Section: Future Application On Sjögren's Syndromementioning
confidence: 99%
“…Since tear fluid contains thousands of proteins -the most abundant of which are lysozyme, lactoferrin, and albumin -noninvasive tear monitoring could also be used to detect protein biomarkers correlated with various diseases. Proteomic analysis of tear samples may be one approach to identify biomarkers linked to ocular diseases (21)(22)(23)(24)(25). However, the correlation between the concentration in tears and blood and its relevance to disease progression must be extensively validated before using novel tear analytes.…”
Section: Advantages Of Tears As a Biomarker Sourcementioning
confidence: 99%
“…Considering the invasive nature of the currently available diagnostic tools, the goal of the work by Aqrawi et al. was to check whether tear fluid could be used for the development of an accurate noninvasive technique to recognize primary SS (pSS) patients. LC‐MS/MS analysis performed on tear fluid from 11 patients and 11 healthy controls resulted in the identification of more than 900 unique peptides (with 75.701 peptide spectrum matches) and of 197 proteins that were significantly upregulated in patients.…”
Section: Bodily Systems and Related Less‐conventional Fluidsmentioning
confidence: 99%