Identification of potential radiation-responsive biomarkers based on human orthologous genes with possible roles in DNA repair pathways by comparison between Arabidopsis thaliana and homo sapiens

Zhao, Lei; He, Xinye; Shang, Yuxuan; Bao, Chengyu; Peng, Ailin; Lei, Xiaohua; Han, Pengcheng; Mi, Dong; Sun, Yeqing

doi:10.1016/j.scitotenv.2019.135076

Cited by 10 publications

(5 citation statements)

References 67 publications

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“…Some previous studies also detected similar enhanced long-chain acylcarnitines, including palmitoylcarnitine (C16:0), sterearoylcarnitine (C18:0), octadecenoylcarnitine (C18:1), and linoleycarnitine (C18:2) in plasma or jejunum tissue after total body irradiation. 11,12,25 Perhaps due to different radiation types, animal models, or sampling times, and these studies were not limited to focus on the carnitine profile alteration, the discovered significant elevated long-chain acylcarnitines were not as numerous as those found in our study. Interestingly, 3 kinds of C16 species levels, including C16:2, C16-OH, and C16:2-OH, were simultaneously enhanced in the small intestine samples and the plasma.…”

Section: Discussioncontrasting

confidence: 49%

Enhancement of Acylcarnitine Levels in Small Intestine of Abdominal Irradiation Rats Might Relate to Fatty Acid β-Oxidation Pathway Disequilibration

et al. 2022

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Objective This study aims to analyze the alteration of carnitine profile in the small intestine of abdominal irradiation-induced intestinal injury rats and explore the possible reason for the altered carnitine profile. Methods The abdomens of 15 male Sprague Dawley (SD) rats were irradiated with 0, 10, and 15 Gy of 60Co gamma rays. The carnitine profile in the small intestine and plasma samples of SD rats at 72 h after abdominal irradiated with 0 Gy or 10 Gy of 60Co gamma rays were measured by targeted metabolomics. The changes of fatty acid β-oxidation (FAO), including the expression of carnitine palmitoyltransferase 1 (CPT1) and acyl-CoA dehydrogenases, were analyzed in the small intestine samples of SD rats after exposed to 0, 10, and 15 Gy groups. Results There were eleven acylcarnitines in the small intestine and fourteen acylcarnitines in the plasma of the rat model significantly enhanced, respectively (P < .05). The expression level and activity of CPT1 in the small intestine were remarkably increased (P < .05), and the activity of acyl-CoA dehydrogenase in the small intestine was noticeably reduced (P < .01) after abdominal irradiation. Conclusion The enhanced acylcarnitine levels in the small intestine of abdominal irradiation rats might relate to the FAO pathway disequilibration.

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Section: Discussioncontrasting

confidence: 49%

Enhancement of Acylcarnitine Levels in Small Intestine of Abdominal Irradiation Rats Might Relate to Fatty Acid β-Oxidation Pathway Disequilibration

et al. 2022

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“…Ionizing radiation also induced DNA double-strand breaks (DSBs) [ 87 ], which can lead to cancers. The genes related to NHEJ were revealed by microarray as radiation-responsive biomarkers [ 88 ]. In our study, we found Oligo-Fucoidan treatment before irradiation in HCC transgenic fish can decrease the expression of genes involved in nuclear outer membrane-endoplasmic reticulum membrane network and NHEJ provide hints for the radioprotection effect of Oligo-Fucoidan in HCC.…”

Section: Discussionmentioning

confidence: 99%

Low Molecular Weight Fucoidan Prevents Radiation-Induced Fibrosis and Secondary Tumors in a Zebrafish Model

Yang

Cheng

et al. 2020

Cancers

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Radiotherapy often causes unwanted side effects such as radiation-induced fibrosis and second malignancies. Fucoidan, a sulfated polysaccharide extracted from brown seaweed, has many biological effects including anti-inflammation and anti-tumor. In the present study, we investigated the radioprotective effect of Oligo-Fucoidan (OF) using a zebrafish animal model. Adult zebrafish of wild-type and transgenic fish with hepatocellular carcinoma were orally fed with Oligo-Fucoidan before irradiation. Quantitative PCR, Sirius red stain, hematoxylin, and eosin stain were used for molecular and pathological analysis. Whole genomic microarrays were used to discover the global program of gene expression after Oligo-Fucoidan treatment and identified distinct classes of up- and downregulated genes/pathways during this process. Using Oligo-Fucoidan oral gavage in adult wild-type zebrafish, we found Oligo-Fucoidan pretreatment decreased irradiation-induced fibrosis in hepatocyte. Using hepatitis B virus X antigen (HBx), Src and HBx, Src, p53−/+ transgenic zebrafish liver cancer model, we found that Oligo-Fucoidan pretreatment before irradiation could lower the expression of lipogenic factors and enzymes, fibrosis, and cell cycle/proliferation markers, which eventually reduced formation of liver cancer compared to irradiation alone. Gene ontology analysis revealed that Oligo-Fucoidan pretreatment increased the expression of genes involved in oxidoreductase activity in zebrafish irradiation. Oligo-Fucoidan also decreased the expression of genes involved in transferase activity in wild-type fish without irradiation (WT), nuclear outer membrane-endoplasmic reticulum membrane network, and non-homologous end-joining (NHEJ) in hepatocellular carcinoma (HCC) transgenic fish. Rescue of those genes can prevent liver cancer formation. Conclusions: Our results provide evidence for the ability of Oligo-Fucoidan to prevent radiation-induced fibrosis and second malignancies in zebrafish.

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“…The results on the effects of SNPs of ERCC1/2 in relation to the efficacy of radiotherapy were conflicting. ERCC1/2 was identified and considered a potential biomarker in response to radiation (16). However, Jin et al (17) developed an OS model and a methodology to identify a biomarker for radiosensitivity in ERCC1/2, and found the ERCC1 rs11615 GG genotype and the ERCC2 rs238406 CC genotype were correlated with radioresistance, and those patients could benefit from higher dose of radiation.…”

Section: Association Between Ercc1/2 Polymorphisms and Rili In Nsclc Patients Who Underwent Definitive Radiotherapymentioning

confidence: 99%

“…There was a trend towards poorer clinical outcomes for patients with the A allele; however, it should be noted that these results were not significant (this may be attribute to the study's small sample size). In relation to ERCC2, only one study has shown that the rs13181 T allele significantly increased tumor recurrence risk in gastric cancer, and it should be noted that some studies have found opposite results (16). In relation to rs1799793, which is described as Asp312Asn in some studies, several pre-clinical studies reported that the Asn/Asn genotype had a suboptimal DNA repair capacity relative to the Asp-containing genotypes.…”

Section: Association Between Ercc1/2 Polymorphisms and Rili In Nsclc Patients Who Underwent Definitive Radiotherapymentioning

confidence: 99%

The association between the ERCC1/2 polymorphisms and radiotherapy efficacy in 87 patients with non-small cell lung cancer

Jiang¹,

Guo²,

Li³

et al. 2021

J Thorac Dis

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Background: This study sought to investigate the association between the ERCC1/2 single-nucleotide polymorphisms (SNPs) and the efficacy of radiotherapy and prognosis in patients with non-small cell lung cancer (NSCLC). Methods: We examined 6 SNPs in the ERCC1 and ERCC2 genes in 87 consecutive patients with NSCLC who were treated with definitive radiotherapy. The objective remission rates (ORR), overall survival (OS), and progressive-free survival (PFS) were assessed. A Cox regression analysis was conducted to analyze the independent factors related to death and recurrence. Result: Patients with the G allele had better OS than patients with the A allele, and there was a statistical difference between the two groups (30.9 vs. 16.2 months; P=0.003). Patients with the AA genotype had significantly worse OS than patients with the AG or GG genotypes (6.8 vs. 19.8 vs. 30.9 months, respectively; P=0.000). The median PFS of the G allele was 18.9 months, which was significantly better than that of the A allele (P=0.040). The median PFS of patients with the GG genotype, the AG genotype, and the AA genotype was 18.9, 11.3, and 5.1 months, respectively; the difference among the three groups was statistically significant (P=0.019). Patients with the G allele also had better PFS than those with the A allele (18.9 vs. 11.3 months, P=0.040). The multivariate cox proportional hazard analysis showed that the ERCC1 gene rs11615 was an independent survival indicator [HR: 1.623, 95% confidence interval (CI): 1.018-2.591, P=0.042] but not an independent recurrence indicator (HR: 1.497, 95% CI: 0.932-2.404, P=0.095). Conclusions: The ERCC1 rs11615 SNP may be a potential biomarker for predicting survival prognosis in Chinese NSCLC patients who have undergone definitive radiotherapy. Patients with the G allele had better OS than those with the A allele.

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Identification of potential radiation-responsive biomarkers based on human orthologous genes with possible roles in DNA repair pathways by comparison between Arabidopsis thaliana and homo sapiens

Cited by 10 publications

References 67 publications

Enhancement of Acylcarnitine Levels in Small Intestine of Abdominal Irradiation Rats Might Relate to Fatty Acid β-Oxidation Pathway Disequilibration

Enhancement of Acylcarnitine Levels in Small Intestine of Abdominal Irradiation Rats Might Relate to Fatty Acid β-Oxidation Pathway Disequilibration

Low Molecular Weight Fucoidan Prevents Radiation-Induced Fibrosis and Secondary Tumors in a Zebrafish Model

The association between the ERCC1/2 polymorphisms and radiotherapy efficacy in 87 patients with non-small cell lung cancer

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