Identification of Potential HLA-A *0201 Restricted CTL Epitopes Derived from the Epithelial Cell Adhesion Molecule (Ep-CAM) and the Carcinoembryonic Antigen (CEA)

Ras, Elisabeth; Burg, Sjoerd H. van der; Zegveld, S. Th.; Brandt, R. M. P.; Kuppen, Peter J. K.; Offringa, Rienk; Warnarr, Sven O.; Velde, C.J.H. Van de; Melief, Cornelis J.M.

doi:10.1016/s0198-8859(97)00032-3

Cited by 43 publications

(25 citation statements)

References 27 publications

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“…Three HLA-A2-restricted Ep-CAM-derived peptides (p 263-271 , p 184 -192 , and p 184 -193 ) have been shown to be immunogenic (4,27,38). In the present study, a number of additional functional MHC class Iand II-restricted Ep-CAM-derived peptides were identified in the context of various HLA alleles.…”

Section: Discussionmentioning

confidence: 99%

Vaccination with Ep-CAM Protein or Anti-Idiotypic Antibody Induces Th1-Biased Response against MHC Class I- and II-Restricted Ep-CAM Epitopes in Colorectal Carcinoma Patients

Mosolits

Markovic

Frödin

et al. 2004

Clinical Cancer Research

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Section: Discussionmentioning

confidence: 99%

Vaccination with Ep-CAM Protein or Anti-Idiotypic Antibody Induces Th1-Biased Response against MHC Class I- and II-Restricted Ep-CAM Epitopes in Colorectal Carcinoma Patients

Mosolits

Markovic

Frödin

et al. 2004

Clinical Cancer Research

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“…In humans, CEA-specific immune responses were also elicited under certain circumstances. It has been shown that CEA contains multiple HLA-restricted CTL epitopes (21)(22)(23)(24)(25)(26). CD8…”

Section: Introductionmentioning

confidence: 99%

Induction of Mucosal and Systemic Immune Responses against Human Carcinoembryonic Antigen by an Oral Vaccine

et al. 2005

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Carcinoembryonic antigen (CEA) is a tumor-associated antigen targeted for the development of colorectal tumor vaccines. In this study, we developed papillomavirus pseudoviruses encoding the truncated CEA without NH 2 -terminal signal peptide (PV-CEA) as an oral vaccine to induce CEAspecific CTL responses. In CEA transgenic (CEA-Tg) mice orally immunized with PV-CEA, the immunologic tolerance to CEA as a ''self-antigen'' was overcome and both mucosal and systemic CEA-specific cytolytic activities were detected by in vitro 51 Cr release assays. In a tumor prevention model, the growth rate of CEA + tumors was significantly delayed in CEA-Tg mice orally immunized with PV-CEA when compared with the control vaccine. Further, the IFN-; enzyme-linked ImmunoSPOT and in vitro 51 Cr release assay results showed that HLA-A2-restricted, CEA-specific CTL responses were induced in both mucosal and systemic lymphoid tissues in A2 transgenic mice after oral immunization with PV-CEA. Finally, we showed that coadministration of papillomavirus pseudoviruses encoding interleukin-2 with PV-CEA enhanced the generation of A2-restricted, CEA-specific CTLs in aged CEA/A2 double transgenic mice, which were more clinically relevant. Our data suggest that PV-CEA pseudovirus vaccine is a promising oral CEA vaccine for humans to induce CEAspecific CTLs at the site of colorectal tumors (i.e., intestinal mucosa), which might efficiently eliminate CEA + colorectal tumor cells in the mucosa. (Cancer Res 2005; 65(15): 6990-9)

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“…We reported 16 genes encoding tumor-associated antigens by cloning from cDNAs of epithelial cancer cells other than colon cancer cells (2)(3)(4)(5)(6)(7)(8), and some of the peptides from these antigens were used as peptide vaccines for colon cancer patients, but no major tumor regression was obtained in this initial clinical trial (9). The other proliferation-related antigens, including breast (HER2/neu) and colon cancer (carcinoembryonic antigen) antigens, were reported to have cytotoxic T lymphocyte (CTL)-directed epitopes (10,11). However, as far as we know, tumor regression was also not obtained in the clinical trials with those peptides.…”

Section: Introductionmentioning

confidence: 99%

Two Proliferation-Related Proteins, TYMS and PGK1, Could Be New Cytotoxic T Lymphocyte-Directed Tumor-Associated Antigens of HLA-A2+ Colon Cancer

Shichijo

Azuma²,

Komatsu³

et al. 2004

Clinical Cancer Research

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Purpose: The purpose of this work was to provide a scientific basis for specific immunotherapy of colon cancer.Experimental Design: This study focused on identification of colon tumor-associated antigens and HLA-A2-restricted and tumor-reactive cytotoxic T lymphocytes (CTLs) generated from tumor-infiltrating lymphocytes of a colon cancer patient. A gene expression cloning method was used to identify genes coding for tumor antigens. Fifty-six peptides with HLA-A2-binding motifs encoded by these proteins were examined for their ability to induce HLA-A2-restricted and tumor-reactive CTLs.Results: We identified the following three genes coding for proliferation-related proteins: thymidylate synthase (TYMS), which is involved in chemoresistance (

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Identification of Potential HLA-A *0201 Restricted CTL Epitopes Derived from the Epithelial Cell Adhesion Molecule (Ep-CAM) and the Carcinoembryonic Antigen (CEA)

Cited by 43 publications

References 27 publications

Vaccination with Ep-CAM Protein or Anti-Idiotypic Antibody Induces Th1-Biased Response against MHC Class I- and II-Restricted Ep-CAM Epitopes in Colorectal Carcinoma Patients

Vaccination with Ep-CAM Protein or Anti-Idiotypic Antibody Induces Th1-Biased Response against MHC Class I- and II-Restricted Ep-CAM Epitopes in Colorectal Carcinoma Patients

Induction of Mucosal and Systemic Immune Responses against Human Carcinoembryonic Antigen by an Oral Vaccine

Two Proliferation-Related Proteins, TYMS and PGK1, Could Be New Cytotoxic T Lymphocyte-Directed Tumor-Associated Antigens of HLA-A2+ Colon Cancer

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