Identification of potential genes that contributed to the variation in the taxoid contents between two Taxus species (Taxus media and Taxus mairei)

Abstract

Taxol is currently a valuable anticancer drug; however, the accumulated mixture of taxoids can vary greatly among Taxus species. So far, there is very little genomic information for the genus Taxus, except for Taxus baccata. Transcriptome analysis is a powerful approach to explore the different regulatory mechanisms underlying the taxoid biosynthesis pathway in Taxus species. First, we quantified the variation in the taxoid contents between Taxus media and Taxus mairei. The contents of paclitaxel and 10-deacetylpaclitaxel in T. media are higher than that in T. mairei. Then, the transcriptome profiles of T. media and T. mairei were analyzed to investigate the altered expressions. A total of 20,704 significantly differentially expressed genes (DEGs), including 9865 unigenes predominantly expressed in T. media and 10,839 unigenes predominantly expressed in T. mairei, were identified. In total, 120 jasmonic acid-related DEGs were analyzed, suggesting variations in 'response to JA stimulus' and 'JA biosynthetic process' pathways between T. media and T. mairei. Furthermore, a number of genes related to the precursor supply, taxane skeleton formation and hydroxylation, and C13-side chain assembly were also identified. The differential expression of the candidate genes involved in taxoid biosynthetic pathways may explain the variation in the taxoid contents between T. media and T. mairei.

Nitric oxide‐mediated regulation of oxidative stress in plants under metal stress: a review on molecular and biochemical aspects

“…Therefore, the development of efficient and simultaneously green‐friendly approaches to reduce metal risks and toxicity in plants is a matter of special interest. Plant growth regulators control various important biological processes at physiological and molecular level (Peleg and Blumwald , Xu et al , Yu et al , Kumar et al , b). Nitric oxide (NO) is regarded as an important signaling compound, inducing a variety of developmental and physiological responses in plants, ranging from germination and senescence, to photosynthesis and redox homeostasis (Nabi et al ).…”

Comparative metabolomic analysis reveals the variations in taxoids and flavonoids among three Taxus species

“…Fresh twigs of each sample were collected from three Taxus species, dried at 40°C for 3 d, and powdered. A modified version of a previously published method was used to prepare crude extracts [ 32]. In brief, 2.0 g powder of each sample was mixed with 30 mL of 100% methanol, and the mixture was subjected to ultrasonication for 60 min.…”

“…Other detailed parameters of the HPLC-MS/MS analysis were as follows: the capillary temperature was 270°C; the ion spray voltage was 3,000 V; the auxiliary gas and sheath gas was N 2 ; and the collision gas was high purity argon. Additionally, the positive electrospray ionization mode was employed and multiple-reaction monitoring was applied for the The separation and determination of eight flavonoids, including quercetin, luteolin, kaempferol, amentoflavone and ginkgetin, were performed according to the same UPLC-MS/MS method described in our paper [ 32].…”

Comparative transcriptomic analyses of powdery mildew resistant and susceptible cultivated cucumber (Cucumis sativus L.) varieties to identify the genes involved in the resistance to Sphaerotheca fuliginea infection

“…Next-generation sequencing is a cost-efficient way to analyze transcriptomic variations under different treatment conditions (Shen et al, 2017;Yu et al, 2017). In 2012, expression profiling of P. cubensis-infected cucumber identified differential expressed genes (DEGs) during the infection process from 1 to 8 d post inoculation (Adhikari et al, 2012).…”