Identification of Plesiomonas spp.: Serological and MALDI-TOF MS methods

Kolínská, Renata; Drevinek, Michal; Aldová, E; Žemličková, Helena

doi:10.1007/s12223-010-0109-3

Cited by 8 publications

(3 citation statements)

References 10 publications

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“…Seventyfour isolates were identified as Plesiomonas shigelloides by biochemical methods and serologically typed prior to analysis by MALDI-TOF MS, with Aeromonas and Shigella isolates being used as outgroups for comparison. A database was constructed de novo, and the authors determined that MALDI-TOF MS was suitable for the identification of Plesiomonas shigelloides, but there was no correlation between the spectral profiles generated and serogrouping results (179).…”

Section: The Enterobacteriaceaementioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology

et al. 2013

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SUMMARY Within the past decade, clinical microbiology laboratories experienced revolutionary changes in the way in which microorganisms are identified, moving away from slow, traditional microbial identification algorithms toward rapid molecular methods and mass spectrometry (MS). Historically, MS was clinically utilized as a high-complexity method adapted for protein-centered analysis of samples in chemistry and hematology laboratories. Today, matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) MS is adapted for use in microbiology laboratories, where it serves as a paradigm-shifting, rapid, and robust method for accurate microbial identification. Multiple instrument platforms, marketed by well-established manufacturers, are beginning to displace automated phenotypic identification instruments and in some cases genetic sequence-based identification practices. This review summarizes the current position of MALDI-TOF MS in clinical research and in diagnostic clinical microbiology laboratories and serves as a primer to examine the “nuts and bolts” of MALDI-TOF MS, highlighting research associated with sample preparation, spectral analysis, and accuracy. Currently available MALDI-TOF MS hardware and software platforms that support the use of MALDI-TOF with direct and precultured specimens and integration of the technology into the laboratory workflow are also discussed. Finally, this review closes with a prospective view of the future of MALDI-TOF MS in the clinical microbiology laboratory to accelerate diagnosis and microbial identification to improve patient care.

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Section: The Enterobacteriaceaementioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology

et al. 2013

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“…Likewise, among the molecular tools focused on the genotyping of isolates for phylogenetic and epidemiological studies are multilocus sequence typing (MLST), random amplified polymorphic DNA (RAPD)-PCR, enterobacterial repetitive intergenic consensus (Eric)-PCR, repetitive extragenic palindromic (REP)-PCR, and pulsed field gel electrophoresis (PFGE) [ 80 , 81 ]. Meanwhile, there are techniques for the identification and phenotypic characterization of bacterial isolates based on the analysis of proteins (mainly ribosomal) through the creation of a specific mass spectrum of genus and species (fingerprint) based on the sensitivity, accuracy, speed, and reproducibility of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) [ 36 , 82 , 83 ].…”

Section: Control and Prevention Of Diseases In Humans Due To Plesiomonas Shigelloidesmentioning

confidence: 99%

Plesiomonas: A Review on Food Safety, Fish-Borne Diseases, and Tilapia

Cortés-Sánchez

Espinosa-Chaurand

Díaz‐Ramírez

et al. 2021

The Scientific World Journal

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Fish and fish products are considered a fundamental part of the human diet due to their high nutritional value. Food-borne diseases are considered a major public health challenge worldwide due to their incidence, associated mortality, and negative economic repercussions. Food safety is the guarantee that foods will not cause harm to the health of those who consume them, and it is a fundamental property of food quality. Food safety can be at risk of being lost at any stage of the food chain if the food is contaminated by pathogenic microorganisms. Many diverse bacteria are present in the environment and as part of the microbiota of food that can be transmitted to humans during the handling and consumption of food. Plesiomonas shigelloides has been mainly associated with outbreaks of gastrointestinal diseases due to the consumption of fish. This bacterium inhabits the environment and aquatic animals and is associated with the microbiota of fish such as tilapia, a fish of importance in fishing, aquaculture, commercialization, and consumption worldwide. The purpose of this document is to provide, through a bibliographic review of databases (Scopus, Web of Science, and Google Scholar, among others), a general informative perspective on food-borne diseases and, in particular, the consumption of fish and tilapia. Diseases derived from contamination by Plesiomonas shigelloides are included, and control and prevention actions and sanitary regulations for fishery products established in several countries around the world are discussed to promote the safety of foods of aquatic origin intended for human consumption and to protect public health.

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“…Also, matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDITOF MS) instrumental methods have been used for proteomics, structural and molecular characterization of P. shigelloides’ lipopolysaccharide (LPS), and serotype (strain) delineation based on the O-PS side chain of LPS-variable chemical units as well as genetic variability in the wb cluster genes [ 11 ]. However, it has been shown that MALDITOF MS characterization of P. shigelloides lacks similarity between the same sero-strains [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization, Intra-Species Diversity and Abundance of Freshwater Plesiomonas shigelloides Isolates

Ekundayo

Okoh

2020

Microorganisms

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Molecular signatures of Plesiomonas shigelloides strain specific to pathogenic and nonpathogenic variants are not well established till present. There is a need for intra-species barcoding of P. shigelloides to aid infection control. This study aims at characterizing and assessing intra-species diversity and abundance of P. shigelloides isolated from three freshwaters in the Eastern Cape Province. The study used a Plesiomonas-specific PCR to characterize the isolates. Intra-species (dis)similarities were assessed using ERIC-PCR and (GTG)5-PCR techniques. The DNA fingerprints produced were electrophoresed, digitized, and documented via computer-assisted pattern analysis. The fingerprints were analyzed using neighbor-joining clustering (NJC) based on Euclidean similarity index. Results revealed 80%, 83.64%, and 80% of the water samples from Tyhume, Kat, and Kubusie rivers, respectively, positive for P. shigelloides isolation. The prevalence of P. shigelloides from sites ranged from 13.5% to 88.9%. NJC delineated 48 isolates to 8 clades (ERIC-fingerprints) and 34 isolates into 7 clades ((GTG)5-fingerprints). The relative abundance of unique strains ranged from 6.3% to 22.9% via the two methods. Both fingerprinting approaches have strain-differentiating potential for P. shigelloides, however ERIC-PCR possessed higher resolution (D = 37.46) advantage over (GTG)5-PCR (D = 29.64). In conclusion, the study achieved intra-species diversity and abundance of P. shigelloides from aquatic milieu and provide further opportunity for intra-species-specific barcoding.

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Identification of Plesiomonas spp.: Serological and MALDI-TOF MS methods

Cited by 8 publications

References 10 publications

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology

Plesiomonas: A Review on Food Safety, Fish-Borne Diseases, and Tilapia

Molecular Characterization, Intra-Species Diversity and Abundance of Freshwater Plesiomonas shigelloides Isolates

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